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Identification of a novel signal for activation of Ti plasmid- encoded vir genes from rice (Oryza sativa L.)

Chinese Science Bulletin 1995年第21期40卷 1824-1828页

作者：施骏许耀刘吉开尹中朝李宝健 Amway (China) Co.Limited The Salk Institute.USA Biotechnology Research Center Zhongshan University Guangzhou 510275 China

virulence.(vir) genes of Ti plasmids code for a specialized conjugation system that mediates the mobilization and transfer of T-DNA from Agrobacterium to the genome of higher plants. This system is activated by a number of plant-specific phenolics, well characterized in dicots, via a two-component regulatory system. In contrast, little is known about the occurrence of vir-inducing signals in monocots, especially in the cereal *** have examined the effects of exudates and extracts from various rice tissues at

关键词： rice signal molecule Agrobacterium tumefaaences Ti plasmid virulence.

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Different infection routes of avian influenza A (H5N1) virus in mice

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Integrative Zoology 2009年第4期4卷 402-408页

作者： Ruiqin SUN Jing LUO Yunying GAO Hongxuan HE National Research Center For Wildlife Born Diseases and Key Laboratory of Animal Ecology and Conservation Biology Institute of ZoologyChinese Academy of SciencesBeijingChina Northwest Agriculture and Forestry University YanglingShanxiChina

The continuing outbreaks of avian influenza A H5N1 virus infection in Asia and Africa have caused worldwide concern because of the high mortality rates in poultry,suggesting its potential to become a pandemic influenza virus in *** transmission route of the virus among either the same species or different species is not yet *** and BABL/c mice were inoculated with the H5N1 strain of influenza A virus isolated from *** animals were inoculated with 0.1 mL 106.83 TCID50 of H5N1 virus oronasally,intraperitoneally and using eye *** viruses were examined by virological and pathological *** addition,to detect horizontal transmission,in each group,healthy chicks and mice were mixed with those *** were detected in homogenates of the heart,liver,spleen,kidney and blood of the infected mice and *** antigen was not detected in the spleen,kidney or gastrointestinal tract,but detected by Plaque Forming Unit(PFU)assay in the brain,liver and lung without degenerative change in these organs(in the group inoculated using eye *** detection results for mice inoculated using eye drops suggest that this virus might have a different tissue tropism from other influenza viruses mainly restricted to the respiratory tract in *** chicken samples tested positive for the virus,regardless of the method of *** influenza A H5N1 viruses are highly pathogenic to chickens,but its virulence.in other animals is not yet *** sum up,the results suggest that the virus replicates not only in different animal species but also through different routes of *** addition,the virus was detection not only in the respiratory tract but also in multiple extra-respiratory *** study demonstrates that H5N1 virus infection in mice can cause systemic disease and spread through potentially novel routes within and between mammalian hosts.

关键词： Avian influenza H5N1 virus inoculation route mice virulence.

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