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Suppression of Breast Cancer Proliferation and Induction of Apoptosis via AKT and ERK1/2 Signal Transduction Pathways by Synthetic Polypeptide Derived from viral macrophage inflammatory protein ⅱ

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2011年第4期31卷 497-503页

作者：杨清玲陈昌杰杨志峰高艳军唐洁 Clinical Testing and Diagnosing Experimental Center of Bengbu Medical College Anhui Key Laboratory of Infection and Immunology

SDF-1α,a ligand for the chemokine receptor CXCR4,is well known for mediating the migration of breast cancer *** a previous study we demonstrated that a synthetic 21-mer peptide antagonist of CXCR4（NT21MP） derived from the viral macrophage inflammatory protein ⅱ could antagonize tumor growth in vivo by inhibiting cellular proliferation and inducing apoptosis in breast cancer ***,the role of SDF-1α in the signaling pathways underlying the proliferation of human breast cancer cells and associated signaling pathways and inhibiting signal pathways of NT21MP remained *** present study investigated the mechanism of NT21MP on anti-tumor in breast cancer in *** effect of NT21MP on the viability of cells was determined by the MTT *** V-FITC and PI staining was performed to detect early stage apoptosisin SKBR3 cells treated with SDF-1α and AMD3100 or *** blotting techniques were used to assay the composition of phosphoproteomics and total proteins present in the SKBR3 breast cancer ***-PCR and Western blotting technique were used to detect the effect of NT21MP and AMD3100 on Bcl-2 and Bax *** results indicated that SDF-1α prevented apoptosis and promoted the proliferation of SKBR3 human breast cancer *** compared with untreated SKBR3 cells,Treatment with SDF-1α significantly increased cell viability,and NT21MP abolished the protective effects of SDF-1α dose-dependently（P0.05）.There was a significant decrease in the percentage of apoptotic cells after SDF-1α treatment as compared with control group（2.7%±0.2% vs.5.7%±0.4%,P0.05）.But pretreatment of SKBR3 cells with NT21MP significantly attenuated the antiapoptotic effects of SDF-1α as compared with SKBR3 cells without NT21MP *** proliferative and anti-apoptotic effects of SDF-1α in SKBR3 cells were associated with an increase in AKT and ERK1/2 phosphorylation as well as a decrease in Bax expression and an increase in Bcl-2 *** change

关键词： viral macrophage inflammatory protein ⅱ CXCR4 proliferation apoptosis AKT

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