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Motor neuron-specific RhoA knockout delays degeneration and promotes regeneration of dendrites in spinal ventral horn after brachial plexus injury

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Neural Regeneration Research 2023年第12期18卷 2757-2761页

作者： Mi Li Jiawei Xu Ying Zou Jialing Lu Aiyue Ou Xinrui Ma Jiaqi Zhang Yizhou Xu Lanya Fu Jingmin Liu Xianghai Wang Libing Zhou Jiasong Guo Department of Histology and Embryology School of Basic Medical SciencesSouthern Medical UniversityGuangzhouGuangdong ProvinceChina Department of Spine Orthopedics Zhujiang HospitalSouthern Medical UniversityGuangzhouGuangdong ProvinceChina Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering Southern Medical UniversityGuangzhouGuangdong ProvinceChina Key Laboratory of Mental Health of the Ministry of Education Guangdong-Hong Kong-Macao Greater Bay Area Center for Brain Science and Brain-Inspired IntelligenceGuangdong Province Key Laboratory of Psychiatric DisordersGuangzhouGuangdong ProvinceChina Guangdong-Hong Kong-Macao Institute of CNS Regeneration Ministry of Education CNS Regeneration Collaborative Joint LaboratoryJinan UniversityGuangzhouGuangdong ProvinceChina

Dendrites play irreplaceable roles in the nerve conduction pathway and are vulnerable to various *** axotomy of motor neurons results in the retraction of dendritic arbors,and the dendritic arbor can be re-expanded when reinnervation is *** is a target that regulates the cytoskeleton and promotes neuronal survival and axon ***,the role of RhoA in dendrite degeneration and regeneration is *** this study,we explored the potential role of RhoA in dendrites.A line of motor neuronal conditional knockout mice was developed by crossbreeding HB9~(Cre+)mice with RhoA~(flox/flox)*** established two models for assaying dendrite degeneration and regeneration,in which the brachial plexus was transection or crush injured,*** found that at 28 days after brachial plexus transection,the density,complexity,and structural integrity of dendrites in the ventral horn of the spinal cord of RhoA conditional knockout mice were slightly decreased compared with that in Cre *** underwent degeneration at 7 and 14 days after brachial plexus transection and recovered at 28–56 *** density,complexity,and structural integrity of dendrites in the ventral horn of the spinal cord of RhoA conditional knockout mice recovered compared with results in Cre *** findings suggest that RhoA knockout in motor neurons attenuates dendrite degeneration and promotes dendrite regeneration after peripheral nerve injury.

关键词： brachial plexus conditional knockout degeneration dendrites motor neuron peripheral nerve injury regeneration RhoA spinal cord ventral horn

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Changes of 5-hydroxytryptamine and tryptophan hydroxylase expression in the ventral horn of spinal cord

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Neuroscience Bulletin 2008年第1期24卷 29-33页

作者： Chuan-Xiang XU Hong-Tao LIU Jing WANG Institute of Healthy and Environmental Medicine Academy of Military Medical Sciences Tianjin 300050 China

Objective To investigate changes of 5-hydroxytryptamine （5-HT） and its synthesis rate-limiting enzyme tryp-tophan hydroxylase （TPH） in the ventral horn of spinal cord after exercise-induced fatigue, and to further discuss the mecha- nism of exercise-induced central fatigue at spinal level. Methods Sixteen healthy adult Wistar rats were randomly divided into 2 groups： exercise-induced fatigue group and control group. Immunohistochemical staining for 5-HT and TPH in the ventral horn were performed and analysized quantitatively. The mean optic densities of 5-HT and TPH positive fibers or terminals were measured by computerized image analyzer. Results Both 5-HT and TPH positive fibers/terminals decreased in the exercise-induced fatigue group. The immunohistochemical staining was weaker and the mean optic densities decreased obviously in the fatigue group compared with those in the control group （P 〈 0.05）. Conclusion 5-HT and TPH in the ventral horn of spinal cord might be involved in exercise-induced fatigue.

关键词： ventral horn spinal cord 5-hydroxytryptamine tryptophan hydroxylase exercise-induced fatigue immunohisto-chemistry

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pSVPoMcat微基因修饰雪旺细胞脊髓内移植对操作脊髓再生的影响

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中国组织工程研究与临床康复 2001年第16期10卷 152-153页

作者： CHEN Li gang General Hospital of Chengdu Military Command First Affiliated Hospital of West China University of Medical Science

Objective In order to observe the role of genetically modified Schwann cell (SC) with pSVPoMcat in the regeneration of injured spinal *** The cells were implanted into the spinal *** SD rats were used to establish a model of hemi- transection of spinal cord at the level of T8,and were divided into three groups,randomly, that is,pSVPoMcat modified SC implantation(Group A), SC implantation(Group B),and without cell implantation as control(Group C).After three months the presence of axonal regeneration of the injured spinal cord was examined by means of horseradish peroxidase(HRP)retrograde labeling technique and *** The results indicated that HRP labeled cells in Group A and B could be found in the superior region of injured spinal cord and the brain stem such as the red nuclei and oculomotor nuclei. The density of ventral horn neurons of the spinal cord and the number of myelinated axons in 100 μ m of the white matter was A >B >C *** In brief,the pSVPoMcat modified SC intraspinal implantation could promote regeneration of the injured spinal cord.

关键词：基因修饰雪旺细胞脊髓内移植操作脊髓再生 spinal cord horseradish peroxidase axonal regeneration ventral horn Schwann cell brain stem In order

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