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Cryopreservation of veliger larvae of Trumpet Shell, Charonia sauliae: an Essential Preparation to Artificial Propagation

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Journal of Ocean University of China 2009年第3期8卷 265-269页

作者： KANG Kyoung Ho ZHANG Zhifeng BAO Zhenmin SHAO Mingyu Department of Aquaculture Chonnam National University Yeosu-city 550-749 South Korea Key Laboratory of Marine Genetics and Gene Resource Exploration of Ministry of Education （MaGGR） Ocean University of China Qingdao 266003 P. R. China

Trumpet shell, Charonia sauliae, is an endangered and valuable species, but its artificial propagation protocol has not been successfully established. To estimate the possibility of cryopreservation for larvae of C. sauliae, which is a potential preparation for its artificial reproduction and further research, in this study a protocoi for the cryopreservation of veliger larvae of trumpet shell was optimized. Through a two-step cryopreservation procedure, four kinds of cryoprotectants （ethylene glycol, 1, 2-propanediol, dimethyl sulfoxide and glycerol） were employed at three concentrations （1.0, 1.5 and 2.0molL^-1） respectively and survival rates of larvae were determined after a storage of lh. The larvae frozen with these four cryoprotectants after 1 h storage were cultured, and then survival rates were determined at 24, 72 and 120 h after thawing. Dimethyl sulfoxide at a concentration of 1.5 molL^-1 showed the best protective effect in all experiments （p〈0.05）. And survival rates of larvae frozen with dimethyl sulfoxide were determined after 1, 7 and 15 d of storage. The survival rates of larvae frozen with 1.5 molL^-1 dimethyl sulfoxide after 1 h, 1 d, 7 d and 15 d of storage were 80.77% ±7.51%, 80.34%±11.28%, 83.10%±9.14% and 77.23%±6.22% respectively. No significant differences in survival rates of larvae frozen with dimethyl sulfoxide were observed after various storage periods （p〉0.05）.

关键词： trumpet shell Charonia sauliae veliger larva cryopreservation

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