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Transcriptome analysis of leaves,roots and flowers of Panax notoginseng on ginsenoside and alkaloid biosynthesis and amelioration of vascular insufficiency conditions by saponins from the flower buds

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中国药理学与毒理学杂志 2015年第S1期29卷 105-105页

作者： Bin-ruiYANG Kwok-KuenCHEUNG Ming-huaLIU Wai-FungCHEUNG XinZHOU Rui-fangXIE You-huaWANG PuiManHOI StephenKwok-WingTSUI SimonMing-YuenLEE State Key Laboratory of Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences University of Macao Macao China Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine Shanghai 200032 China Department of Rehabilitation Sciences Hong Kong Polytechnic University Hong Kong China School of Biomedical Sciences Chinese University of Hong Kong Hong Kong China

OBJECTIVE To investigate the transcriptomic details on the biosynthetic pathways in different parts of the Panax notoginseng and the pharmacological activity of the saponins extracted from the flower(FS)on vascular insufficiency *** RNA sequencing of three different Panax notoginseng tissues was performed using next generation DNA sequencing and differential gene expression was validated by real-time *** order to determine pro-angiogenic and therapeutic effects of FS on myocardial infraction(MI),FS was examined on the endothelial cell migration assay,vascular insufficiency model in zebrafish and MI model in *** After assembling the high quality sequencing reads into 107 340 unigenes,biochemical pathways were predicted and 9 908 unigenes were assigned to 135 KEGG *** them,270 unigenes were identified to be involved in triterpene saponin biosynthesis as well as 350 and 342unigenes were predicted to encode cytochrome P450 sand glycosyltransferases,*** unigene was annotated as CYP716A53v2,probably participates in the formation of protopanaxatriol from protopanaxadiol and the differential expression of this gene was confirmed by real-time *** addition,the pharmacological evaluation demonstrate that FS significantly promoted vascular endothelial growth factor(VEGF)induced the migration of human umbilical vein endothelial cells(HUVECs)and partially restored defective intersegmental vessels in a chemically induced vascular insufficiency model of zebrafish ***,the two week posttreatment of the rat MI model with FS(25-50mg·kg-1·d-1)induced approximately 3-fold upregulation of VEGF mRNA expression,with a concomitant increase in blood vessel density in the peri-infarct area of the heart by 50.7%,compared to 41.4%in the MI ***,TUNEL analysis indicates a reduction in the mean apoptotic nuclei per field in peri-infarct myocardium upon FS *** We have established a global transcriptome da

关键词： Panax notoginseng transcriptome ginsenosides vascu

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