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H_2 PRODUCTION BY ANABAENA variabilis MUTANT IN COMPUTER CONTROLLED TWO-STAGE AIR-LIFT TUBULAR PHOTOBIOREACTOR

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Chinese Journal of Oceanology and Limnology 2000年第2期18卷 126-131页

作者：刘建国 D.O.Hall K.K.Rao A.A.Tsygankov D.A.Sveshnikov Institute of Oceanology Chinese Academy of Sciences Qingdao China Division of Life Sciences King's College London London UK Institute of Solid Science and Photosynthesis Russian Academy of Sciences Pushchino Moscow Region Russia Dept. of Plant Physiol. Biological Faculty Moscow State University Moscow Russia

A 4.34 liter two stage air lift photobioreactor incorporating Anabaena variabilis ATCC29413 mutant PK84 was used to study H 2 production. Results showed that H 2 production increased with increasing light intensity from 47 μE/(m 2·s) up to 190 μE/(m 2·s), but that further increase of light intensity decreased the H 2 production because of the inhibition due to the high pO 2. The data also indicated that longer argon gas charge resulted in more H 2 produced due to the increase of nitrogenase activities and heterocyst frequency, and that more than 1.3 L net H 2 was produced from this computer controlled photobioreactor.

关键词： H 2 production Anabaena variabilis photobioreactor light intensity

小球藻Chlorella variabilis NC64A二酰甘油酰基转移酶基因的克隆表达与功能研究

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北京大学学报（自然科学版） 2016年第2期52卷 187-192页

作者：杨金水高全秀李兆胜邢冠岚袁红莉中国农业大学生物学院农业生物技术国家重点实验室北京100193

为了揭示二酰甘油酰基转移酶(DGAT)在小球藻油脂合成过程中的作用,对单细胞小球藻Chlorella variabilis NC64A的二酰甘油酰基转移酶进行原核克隆表达及功能初步研究。结果表明,其编码序列为894bp,编码297个氨基酸,表达蛋白的表观分子量... 详细信息

为了揭示二酰甘油酰基转移酶(DGAT)在小球藻油脂合成过程中的作用,对单细胞小球藻Chlorella variabilis NC64A的二酰甘油酰基转移酶进行原核克隆表达及功能初步研究。结果表明,其编码序列为894bp,编码297个氨基酸,表达蛋白的表观分子量为33 k Da,p I 9.48。保守结构域分析表明,该蛋白属于Lysophospholipid acyltransferases(LPLATs)超家族,具有二酰甘油酰基转移酶活性,序列位点H68,L71,F76,R94,I97和GAA(144?146)组成特定的酰基受体结合口袋,能够结合酰基?酰基载体蛋白(ACP)或者酰基辅酶A上的酰基,催化三酰甘油合成的最后一步。表达蛋白与Ss PDAT和At PDAT的序列相似性分别为32%和24%,表明该蛋白可能具有磷脂酰甘油酰基转移酶(PDAT)活性,能利用磷脂上的酰基合成三酰甘油。因此,采用薄层层析方法以L-α-磷脂酰胆碱和1,2-二油酰-sn-甘油为底物,检测到其确实具有PDAT活性,表明限氮条件下NC64A中DGAT的PDAT活性可能促进了膜脂降解耦合三酰甘油的合成。

关键词： Chlorella variabilis 三酰甘油二酰甘油酰基转移酶磷脂酰甘油酰基转移酶克隆表达

A heterozygous mutation in GJA1 gene in Chinese family with serious erythrokeratodermia variabilis et progressive

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Chinese Medical Journal 2019年第1期132卷 86-88页

作者： Bi-Rong Guo Hai-Bin Cai Wen-Kai Zong Cong-Sheng Li Li-Zhong Liu Song Qiao Qi-Ming Zhu Ming Li Department of Dermatology Third Affiliated Hospital of Anhui Medical UniversityFirst People's Hospital of HefeiHefeiAnhui 230061China Department of Dermatology People's Hospital of Pudong New AreaShanghai 201200China Department of Dermatology Institute of DermatologyChinese Academy of Medical Sciences and Peking Union Medical CollegeNanjingJiangsu 210042China Department of Emergency Third Affiliated Hospital of Anhui Medical UniversityFirst People's Hospital of HefeiHefeiAnhui 230061China Department of Dermatology Xinhua HospitalShanghai Jiaotong University School of MedicineShanghai 200092China

To the Editor:Erythrokeratodermia variabilis (EKV;MIM 133200) is characterized by the coexistence of transient,figurate,erythematous patches,and localized keratotic *** shows marked phenotypic heterogeneity,even within kindreds bearing the same diseasecausing mutation.[1] The term EKV et progressiva (EKVP) is a severe variant of EKV and has been proposed to encompass the diversity of phenotypes,ranging from limited hyperkeratotic plaques and erythematous patches to severe progressive symmetric erythrokeratodermia which can feature more generalized cutaneous involvement.

关键词： Erythema dermatitis Erythrokeratodermia variabilis (EKV MIM 133200) The term EKV et progressiva (EKVP)

Erythrokeratoderma variabilis in a 3-year-old child:a satisfactory response to treatment with oral acitretin and topical 0.025% tretinoin cream

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International Journal of Dermatology and Venereology 2018年第4期1卷 232-234页

作者： Yan Teng Jian-Fang Sun Yi Liu Clinical Trials and Cosmetics Testing Center Hospital for Skin Diseases (Institute of Dermatology) Chinese Academy of Medical Sciences and Peking Union Medical College Nanjing 210042 Jiangsu China Department of Pathology Hospital for Skin Diseases (Institute of Dermatology) Chinese Academy of Medical Sciences and Peking Union Medical College Nanjing 210042 Jiangsu China

Introduction Erythrokeratoderma variabilis is a rare autosomal dominant genodermatosis that usually appears within the first year of life, but may arise later in childhood. EKV was described by Mendes da Costa in 1925(1)Clinical characteristics of EKV consist of irregularly-shaped, erythematous, scaly patches and hyperkera-totic plaques.

关键词： Erythrokeratoderma variabilis a satisfactory response

土耳其海绵Ircinia variabilis中溴代生物碱的分离与鉴定

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中南药学 2013年第3期11卷 185-186页

作者：赵晶晶刘华钢广西医科大学药学院广西南宁530021 广西食品药品监督管理局广西南宁530022

目的研究土耳其海绵Ircinia variabilis的化学成分。方法采用多种色谱方法分离纯化,依据理化性质、波谱数据和文献对照的方法鉴定结构。结果从土耳其海绵Ircinia variabilis提取物中分离鉴定了5个溴代生物碱类化合物:fistularin-3(1),2-... 详细信息

目的研究土耳其海绵Ircinia variabilis的化学成分。方法采用多种色谱方法分离纯化,依据理化性质、波谱数据和文献对照的方法鉴定结构。结果从土耳其海绵Ircinia variabilis提取物中分离鉴定了5个溴代生物碱类化合物:fistularin-3(1),2-oxazolidinone(2),(E)-1-acetamide-3,5-dibromo-4-ethoxy-4-methoxy-1-hydroxy-2,5-cyclohexaddienone(3),(Z)-1-acetamide-3,5-dibromo-4-ethoxy-4-methoxy-1-hydroxy-2,5-cyclohexaddienone(4),3,5-dibromotyrosine(5)。结论化合物1从该种内首次分离得到,化合物2～4从该种属中首次分离得到。

关键词：海绵 Ircinia variabilis 溴代生物碱结构鉴定

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宝天曼落叶阔叶林样地栓皮栎种群空间格局

生物多样性 2011年第2期19卷 224-231页

作者：袁志良王婷朱学灵沙迎迎叶永忠河南农业大学生命科学学院郑州450002 河南农业大学林学院郑州450002 河南省宝天曼自然保护区内乡474350

种群空间格局分析有利于更好地理解格局形成的潜在生态过程。本研究在温带—亚热带过渡区的宝天曼自然保护区内选择两个1ha固定监测样地,以栓皮栎(Quercus variabilis)为研究对象,采用单变量和双变量g(r)函数分析了两个典型样地中栓皮... 详细信息

种群空间格局分析有利于更好地理解格局形成的潜在生态过程。本研究在温带—亚热带过渡区的宝天曼自然保护区内选择两个1ha固定监测样地,以栓皮栎(Quercus variabilis)为研究对象,采用单变量和双变量g(r)函数分析了两个典型样地中栓皮栎种群空间分布格局、4个不同生长阶段的空间分布格局和空间关联性,以及栓皮栎活立木与残干间的空间关联性。结果表明:(1)栓皮栎种群分布格局以聚集为主,样地I的聚集性更强,而样地II则表现出较大的随机性;(2)在两个样地中,早期阶段的个体均表现为聚集分布格局,而后期阶段的个体则表现为随机分布格局;(3)样地I中栓皮栎种群不同生长阶段均表现为空间正相关,样地II中栓皮栎种群的早期阶段与其后的两个阶段间均表现为空间负相关;(4)样地I中栓皮栎活立木与残干之间为空间正相互作用,样地II中栓皮栎活立木与残干之间无空间关联性。宝天曼自然保护区栓皮栎种群空间分布格局可能受环境异质性的作用,并对自身的天然更新产生影响。

关键词：空间格局 Quercus variabilis 宝天曼自然保护区

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陕西商南县栓皮栎林分评价与管理(英文)

长江科学院院报 2012年第11期29卷 16-21页

作者：王小兰 Hany El Kateb Bernhard Felbermeier 张平仓 Reinhard Mosandl Institute of Silviculture Technische Universitt München Soil and Water Conservation Department Yangtze River Scientific Research Institute

The present study was conducted within the frame of the Sino-German project "Rehabilitation of degraded land ecosystems in the mountainous area of the Southern Shaanxi Province,China".The study deals with the assessment of the potential of oak coppices as well as the evaluation of the socioeconomic conditions in the Shangnan *** ultimate objective of the study is to provide recommendations on the sustainable management of forest resources,which does not only aim to improve the environmental situation but also to satisfy the demands of the local rural *** study was based on 30 samples of oak coppices stands,which were randomly selected within an area of a size of 20 km × 20 *** each selected stand,the mature stand and understory regeneration were investigated in *** the socio-economic survey,175 households from 11 villages were randomly *** revealed that the stocking capacity of the cork oak coppices reaches 120 m3/ha in average at the older age classes(≥25 years) even after frequent timber *** potential for productivity was indicated by the availability of sufficient vigorous individuals at different age *** addition,the understory regeneration was sufficient in density(19,000±133 individual/ha) and consisted of diverse valuable native-species(17 species within a survey area of 400 m2).The results of the assessment of the oak coppices provided possibilities for some practices that can be recommended towards sustainable management of such *** the other hand,results of the socioeconomic study showed a high degree of acceptance among the local inhabitants(79% of the total households) to change traditional land use,providing an enhancement of their economic situation.

关键词： Quercus variabilis coppice understory regeneration southern Shaanxi Province

Structure and function of PBS model complex

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Chinese Science Bulletin 1998年第8期43卷 646-649页

作者： Hui Wang Jingquan Zhao Lijin Jiang Chinese Acad Sci Inst Photog Chem Beijing 100101 Peoples R China

A complex of phycobiliproteins, containing phycoerythrocyanin (PEC), C_phycovyanin (C_PC) and allo_phycocyanin (APC) as well as some linker polypeptides, was reconstructed. The absorption and fluorescence spectra of the complex were compared with those of native phycobilisomes (PBS) and the phycobiliproteins. Based on the measured data, it can be concluded that the complex can be taken as a model of PBS and is an entirely functional group for excitation energy transfer step by step from peripheral PEC to APC. The single terminal emitter feature of the complex makes it favorable for clarifying energy transfer pathways and the kinetics in comparison with native PBS. Further research is carried on in the lab.

关键词： Anabaena variabilis PBS model complex reconstruction excitation energy transfer.

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中条山栓皮栎林数量分类研究

林业科技通讯 2019年第9期 3-8页

作者：孙剑车鑫贵吴应建山西省中条山国有林管理局

基于群落学野外调查,应用TWINSPAN和DCA分别对中条山太宽河自然保护区栓皮栎(Quercus variabilis)林进行了数量分类和排序研究。结果表明:将中条山太宽河栓皮栎林的42个样方分为8个群丛,分别是:I.栓皮栎+槲栎-陕西荚蒾+连翘-披针叶苔草... 详细信息

基于群落学野外调查,应用TWINSPAN和DCA分别对中条山太宽河自然保护区栓皮栎(Quercus variabilis)林进行了数量分类和排序研究。结果表明:将中条山太宽河栓皮栎林的42个样方分为8个群丛,分别是:I.栓皮栎+槲栎-陕西荚蒾+连翘-披针叶苔草群丛,Ⅱ.栓皮栎+橿子栎-连翘+卫茅-披针叶苔草群丛,Ⅲ.栓皮栎+板栗-连翘-披针叶苔草群丛,Ⅳ.栓皮栎+板栗-连翘-披针叶苔草+矛叶荩草群丛,Ⅴ.栓皮栎-黄栌+连翘-披针叶苔草+野青茅群丛,Ⅵ.栓皮栎-黄栌-披针叶苔草群丛,Ⅶ.栓皮栎+槲栎-荆条+连翘+黄栌-披针叶苔草+委陵菊群丛,Ⅷ.栓皮栎+槲栎-连翘+榛子-披针叶苔草+细叶沙参群丛。群落和优势种的DCA排序结果表明:影响群落分布和优势种分布的生态因子主要是热量和水分。

关键词：栓皮栎 Quercus variabilis 群丛分类排序