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Toxoplasma gondiiBut Not Leishmania majoror Trichomonas vaginalisDecreases Cell Proliferation and Increases Cell Death on Fibrosarcoma Cancer Cells in Culture Medium

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World Journal of Vaccines 2012年第2期2卷 105-108页

作者： Hedayatollah Shirzad Soleiman Khorami Narges Soozangar Mortaza Yousefi Hossein Yousofi Darani Cell and Molecular Research Center Department of Immunology Faculty of Medicine Shahrekord University of Medical Sciences Shahrekord Iran Department of Parasitology Cell and Molecular Research Center Faculty of Medicine Shahrekord University of Medical Sciences Shahrekord Iran Student Research Committee Shahrekord University of Medical Sciences Shahrekord Iran

It has been shown that some parasites interfere with malignant tumor cells growth in vivo or in vitro. In this work anticancer activity of three live protozoan parasites;Toxoplasma gondii, Trichomonas vaginalis and Leishmania major on fibrosarcoma cells growth has been investigated in cell culture medium. In this experimental study, WEHI-164 fibrosarcoma cells treated with alive Toxoplasma gondii tachyzoite, Trichomonas vaginalis trophozoite or Leishmania major promastigote as case groups or left intact as control groups. Following 24 hours incubation the number of cells, lactate dehydrogenase (LDH) and apoptosis were determined in case and control groups. Toxoplasma gondii tachyzoite decreased cell proliferation and increased cell lyses’ but it did not induce apoptosis. Trichomonas vaginalis or Leishmania major didn’t show any effects on cell proliferation, cell lyses or apoptosis. Therefore Toxoplasma gondii may have anticancer activity and further works is recommended to understand the mechanisms of action.

关键词： Toxoplasma Gondii Trichomonas vaginalis Leishmania Major Fibrosarcoma

Frequency and molecular diagnosis of trichomoniasis in symptomatic women referred to laboratories in urmia north west Iran

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Journal of Acute Disease 2017年第4期6卷 175-180页

作者： Elham Shahnazi Habib Mohammadzadeh Chiman Daneshyar Alireza Chavoshin ShahramKhademvatan Cellular and Molecular Research Center&Department of Medical Parasitology and Mycology Urmia University of Medical ScincesUrmia Iran Department of Medical Entomology School of Public HealthUrmia University of Medical SciencesUrmiaIran

Objective:To obtain the frequency of trichomoniasis and molecular diagnosis among women referred to a laboratory in Urmia (north west Iran).Methods: In the cross sectional study during the year 2014-2015, women with complaints of abnormal discharge, itching, irritation and unpleasant odor were studied using convenience sampling. These people were referred to one of the laboratories in town by gynecologist. After preparing the checklist of underlying data, vaginal wet smear samples was prepared to assess andChi-square in terms ofTrichomonas vaginalis(T. vaginalis) infection was examine. And results were analyzed with statistical tests. Results: 11.1% of referred casese were 30-40 years old, and about 54.8% of referred were illiterate and primitive. The frequency of trichomoniasis was 11.1% and statistically there was no significant difference. There was no significant difference among the age, education level, method of contraception, marriage age, location, employment status and the number of delivery withT. vaginalis.Conclusion:T. vaginalis compared to other studies have a moderate prevalence among women of Urmia. Because of the concordance of this infection with other sexual transmitted diseases, it is recommended that further studies of the prevalence of these diseases in Urmia be done.

关键词： Trichomonas vaginalis Frequency Vaginitis Iran

Management of Ovarian Hernia in Children, in Teaching Hospital of Bouaké, Côte d’Ivoire Prise en Charge des Hernies de l’ovaire de l’enfant au CHU de Bouaké, Côte d’Ivoire

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Open Journal of Obstetrics and Gynecology 2018年第13期8卷 1438-1444页

作者： Nandiolo K. Rose Menin Messou Kpangni Jean Bertrand Yenan John Patrick Lohourou Grah Franck University Alassane Ouattara Bouake Cote d’Ivoire Catholic Hospital St Joseph Moscati Yamoussoukro Cote D’Ivoire Department of Gynecology and Obstetrics Teaching Hospital of Bouake Bouake Cote d’Ivoire Department of Paediatric Surgery Teaching Hospital of Bouake Bouake Cote d’Ivoire Department of Pediatrics Teaching Hospital of Bouake Bouake Cote d’Ivoire

Objective: To analyze our experience in the management of ovarian hernia in children. Methods: It was a retrospective study about 11 cases treated in pediatric surgery unit of the Teaching Hospital of Bouaké, in 2 years period. Results: Ovarian hernia represented 7.69% of the hernias and 73.33% of the hernia cases in girls. The mean age was 4 years and the median 36 months. The main reason for consultation was inguinal swelling (45.45%) following by swelling of the labia majora (36.36%), and a round mass of labia majora palpated by parents while bathing the child (18.18%). Nine (9) patients had been operated on;we realize the closure of peritoneo-vaginal canal, associated in 66.66% cases with a routine surgical exploration of the contralateral side. Average age of these children with contralateral hernia was 20 months. The mean follow up was 6 months (15 days - 15 months). We noticed an appearance of contralateral ovarian hernia in a girl operated on at 3 years old without routine surgical exploration. Conclusion: We recommend early management with a systematic surgical exploration of the contralateral side in young female child. A preoperative ultrasonography could be a routine in case of strangulated hernia, if available.

关键词： Ovarian Hernia Children Processus vaginalis

Vaginal Discharge in the Prostitutes of the Group Yèrêlon of Bobo-Dioulasso: Epidemiological, Clinical and Etiological Aspects

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Open Journal of Obstetrics and Gynecology 2017年第8期7卷 871-879页

作者： Bambara Moussa Ouedraogo Jean Louis Mansour Niang Diallo Abdoul Azize Zampaligre Idrissa Gynécologue Bobo-Dioulasso Burkina Faso

The authors report a cross-sectional descriptive study over 6 years, from December 8th, 2003 to October 27th, 2009, involving 911 women involved in the sex trade within the Yèrêlon group in Bobo-Dioulasso. Objectives: To describe the epidemiological, clinical and etiological aspects of vaginal discharge in women in the Yèrêlon group of the city Bobo-Dioulasso. Results: Frequency of vaginal discharge was 48.89%. The mean age was 28.4 years with extremes of 16 and 54 years. Single, divorced and widowed women accounted for 78.8% of the study population. Women who never attended school and those with primary education accounted for 74.3% of the study population. HIV serology was positive in 38.4% of cases. The main symptoms were genital itching, dyspareunia and urinary signs. The main germs identified in the laboratory were Candida albicans, Trichomonas vaginalis and Gardenerella vaginalis. Conclusion: vaginal discharge was found in 48.89% of women in the Yèrêlon group of Bobo-Dioulasso. The clinical study of the discharge and the laboratory results allowed a diagnosis and a better management of the leucorrhea in the group Yèrêlon.

关键词： Leucorrhea Sex Trade Candida albicans Trichomonas vaginalis Gardenerella vaginalis

Nugent Scores of Pregnant Women in a Tertiary Institution in Nigeria

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Advances in Microbiology 2012年第4期2卷 531-536页

作者： Grace Ajani Oyin Oduyebo Muyideen Haruna Charles Elikwu Babcock University Ilishan-Remo Nigeria College of Medicine University of Lagos Lagos Nigeria

Background: Bacterial Vaginosis (BV) is an imbalance of the vaginal normal flora, and it is asymptomatic in most women. Therefore, one of the major ways of diagnosing BV is demonstration of an abnormal flora in the vagina. This can be achieved through various methods including Nugent score. In view of the association of BV with obstetric/gynaecological complications and high rate of HIV transmission, it is desirable to know the prevalence of BV in pregnant women. Knowledge of risk factors will also help to design preventive measures. Methodology: A prospective study was performed to determine the prevalence of BV among pregnant women. High vaginal swabs (HVS) were collected and smears made were Gram stained and scored by the Nugent method. A predesigned questionnaire was also used to obtain information on possible risk factors. Associations between categorical variables were assessed using chi square test and fisher’s exact test as appropriate. Result: Three hundred married women aged between 18 and 45 years were studied. The prevalence of BV was 64.3%, 25% had intermediate and 10.7% had negative Nugent scores. BV rates were high across all age groups, educational background, ethnicity, parity, HIV status, and occupation. The occurrence of BV was higher (75.5%) among gestational age group ≤ 30 weeks (p 0.05) and in women who engaged in unskilled labour (p 0.05). Conclusion: Bacterial vaginosis is a very common condition among the studied population. This high prevalence may necessitate routine screening and treatment during antenatal care in preventing the associated adverse effects of BV during pregnancy and childbirth.

关键词： Bacterial Vaginosis Preterm Labour Pelvic Inflammatory Disease Lactobacillus Garneralla vaginalis Mobiluncus spp

Antiprotozoal Activity of a Thymus vulgarisMethanol Extract and Its Fractions

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Health 2017年第7期9卷 1081-1094页

作者： Jesús Norberto Garza-González Javier Vargas-Villarreal Ma Julia Verde-Star Catalina Rivas-Morales Azucena Oranday-Cárdenas Magda E. Hernandez-García Laura De La Garza-Salinas Francisco González-Salazar Department of Chemistry Phytochemistry and Analytical Chemistry Lab Faculty of Biological Sciences University Autonomous of Nuevo Leon San Nicolas de los Garza Mexico Department of Basic Sciences Division of Health Sciences University of Monterrey San Pedro Garza Garcia Mexico Laboratory of Biochemistry and Cell Physiology Division of Cellular and Molecular Biology Center for Biomedical Research of the Northeast Mexican Social Security Institute Monterrey Mexico Mexican Social Security Institute Nuevo Leon Delegation Monterrey Mexico

Introduction: Thymus vulgaris is used in traditional medicine to treat gastrointestinal diseases because of its antifungal, antibacterial, and antispasmodic activity. Objective: To verify whether Thymus vulgaris also has antiprotozoal activity against Trichomonas vaginalis, Giardia lamblia and Entamoeba histolytica trophozoites. Materials and methods: Conventional cultures of parasites were measured on the third day during the logarithmic growth phase. The antiprotozoal activity of the methanol extract and its fractions were evaluated comparing growth in cultures with and without extracts. Next, the extract was fractionated by polarity-based partitioning. Then, the purity of each fraction was determined by thin layer chromatography (TLC). The percentage of growth inhibition was calculated with respect to untreated controls. The 50% inhibitory concentration (IC50) of each extract was calculated by PROBIT analysis. Results: We found that a methanol extract of the aerial parts of Thymus vulgaris, at 300 μg/mL, inhibited the in vitro growth of G. lamblia and T. vaginalis, while E. histolytica growth was poorly inhibited. The methanol extract was further separated into mixtures of ursolic, oleanolic, and betulinic acids. The IC50 values of ursolic acid against G. lamblia and T. vaginalis were 8.12 μg/mL and 5.51 μg/mL, respectively. Conclusions: The methanol extract fraction containing ursolic acid obtained from Thymus vulgaris has antiprotozoal activity against G. lamblia and T. vaginalis trophozoites.

关键词： Thymus vulgaris Trichomonas vaginalis Giardia lamblia Entamoeba histolytica Antiprotozoal Agents