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Effects of feeding time on complement component C7 expression in Pelteobagrus vachellii subject to bacterial challenge

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Journal of Oceanology and Limnology 2018年第6期36卷 2358-2367页

作者： SHAO Ting QIN Chuanjie DUAN Huiguo YUAN Dengyue WEN Zhengyong WANG Jun GE Fanglan College of Life Science Sichuan Normal University College of Life Science Neijiang Normal UniversityKey Laboratory of Sichuan Province for Fishes Conservation and Utilization in the Upper Reaches of the Yangtze River

Shifting the feeding time for fish from daytime to nighttime could alter their digestive behavior, disturb their metabolism, and may af fect immune-related genes. This study aimed to clone complement component C7 and analyze the different expression of C7 mRNA in fish fed during either the day or at night and then challenged with Aeromonas hydrophila infection. The P v-C7 cDNA of Pelteobagrus vachellii contained 2 647 bp with an open reading frame encoding a protein of 818 amino acids. Multiple sequences analysis indicated that P v-C7 included eight domains, which was similar to results for other species. Quantitative PCR analysis showed that P v-C7 was mainly expressed in the liver, spleen, intestine and head kidney tissues of healthy P. vachellii. Quantitative PCR analysis showed that C7 mRNA transcript in the liver, spleen and head kidney also increased significantly when the fish were fed at nighttime(20:00). In addition, the expression of Pv-C7 mRNA significantly increased with A. hydrophila challenge in the liver(48–96 h), spleen and head kidney(12–96 h) tissues of P. vachellii. Pv-C7 mRNA expression of the fish fed at nighttime showed significant higher than that in the fish fed at day time at 12–48 h in head kidney and 12–24 h in spleen. This study indicates that altering the feeding time from daytime to nighttime could increase P v-C7 mRNA expression, and feeding time may af fect the immune response involving C7.

关键词： Pelteobagrus vachellii complement component C7 shifting feeding time clock

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