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Anti-inflammatory Effects and Mechanisms of usnic acid

Journal of Wuhan University of Technology(Materials Science) 2011年第5期26卷 955-959页

作者：黄志军阮金兰 College of Pharmacy Tongji Medical CenterHuazhong University of Science and Technology School of Chemical Engineering Wuhan University of Technology

The anti-inflammatory effect and mechanism of usnic acid （UA） were explored on lipopoly-saccharide （LPS）-stimulated RAW264.7 cell *** effects of UA on pro-inflammatory cytokines including tumor necrosis factor-alfa （TNF-α）,interleukin-6 （IL-6） and interleukin-1 beta （IL-1β）,pro-inflammatory mediators such as nitric oxide （NO）,inducible nitric oxide synthase （iNOS） and cyclooxygenase-2 （COX-2） were studied by sandwich ELISA,real-time PCR and western blot ***,the effect of UA on anti-inflammatory cytokine interleukin-10 （IL-10） and anti-inflammatory mediator heme oxygenase-1 （HO-1） were also studied following the same ***,nuclear factor-κB （NF-κB） was assayed by *** results showed that UA has anti-inflammatory effect by down-regulatinng iNOS,COX-2,IL-1β,IL-6 and TNF-α,COX-2 gene expression through the suppression of NF-κB activation and increasing anti-inflammatory cytokine IL-10 and anti-inflammatory mediator HO-1 production.

关键词： usnic acid anti-inflammation western blot analyses immunocytochemistry

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EFFECT OF usnic acid ON TNF-α AND NO PRODUCTION IN LIPOPO-LYSACCHARIDE-STIMULATED MACROPHAGES

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Journal of Pharmaceutical Analysis 2006年第2期18卷 153-156页

作者：靳菊情贺浪冲李翠芹 Medical School of Xi'an Jiaotong University Xi'an 710061 China

Objective To investigate the molecular mechanisms that are responsible for anti-inflammatory effect of usnic acid (UA), the effects of UA from usnea longissm on tumor necrosis factor-α(TNF-α) and nitric oxide (NO) production in peritoneal macrophages has been examined. Methods The different concentrations of UA were added to peritoneal macrophages. The TNF-α and NO production in peritoneal macrophages were examined with mouse TNF-α ELISA kit and NO content by measuring the amount of nitrite (NO-_2μmol/L) formed in the medium using Griess reaction. The activity of inducible nitric oxide synthase (i-NOS) was determined using i-NOS detection kit and the TNF-α mRNA expression was tested by reverse transcriptase polymerase chain reaction (RT-PCR). Results UA decreased the TNF-α and NO level in LPS-stimulated peritoneal macrophages in dose-dependent manner, the IC_ 50 values were 12.8μmol/L and 5.7μmol/L respectively. RT-PCR analysis indicated that UA could inhibit TNF-α mRNA expression; the activity analysis of i-NOS indicated that UA could inhibit the activity of i-NOS. Conclusion UA could inhibit the TNF-α and NO production in peritoneal macrophages, it may be associated with the anti-inflammatory activity of UA.

关键词： usnic acid macrophage tumor necrosis factor nitric oxide

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THE　ULTRASTRUCTURE　OF　TOXOPLASMA　GONDII　TACHYZOITES　AND　THE　EFFECT　OF　usnic　acid　ON　IT

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Journal of Pharmaceutical Analysis 1996年第1期10卷 91-94页

作者：吴杰杨少毅程彦斌

The normal fine structures of toxoplasma gondii tachyzoite and the erfect of usnic acid on the ultrastructure of the tachyzoites were observed by transmission electron microscope (TEM).The studies indicate that the pathological changes or the ultrastructure of the parasite took place under the effect of the 10 mg/L usnic acid for 30 *** changes can be illustrated as folio'vs; 1)The posterior portion of the rhopties were destroyed.2)The membrane of the daughter cell fractured. 3)The membranate organellae or the organisms were demaged.

关键词： toxoplasma gondii tachyzoite ultrastructure usnic acid

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