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universal probe with oriented antibody to improve the immunochromatographic assay of lead ions in Procambarus clarkii

引用

Food Quality and Safety 2023年第2期7卷 309-316页

作者：李杨阳朱正伟曲文莉杨庆刘言王桥段烁伍金娥宫智勇许琳 College of Food Science and Engineering Wuhan Polytechnic UniversityWuhanChina Hubei Provincial Institute for Food Supervision and Test WuhanChina Key Laboratory for Deep Processing of Major Grain and Oil Ministry of EducationWuhanChina Hubei Key Laboratory for Processing and Transformation of Agricultural Products WuhanChina Food Safety Research Center Key Research Instituteof Humanities and Social Sciences of Hubei ProvinceWuhanChina

Objectives:Based on the information from the random inspection of foods by the China Food and Drug Administration in 2022,the contamin-ation levels of lead ions are high in many edible *** methods of detecting lead ions cannot meet the requirements of on-site analysis of food due to the need for large *** immunochromatographic assay(iCA)is an effective,rapid,on-site analytical technique for determining lead ions in ***,the performance of ICA based on the traditional probe(AuNP-mAb)is limited by ignoring the influ-ence of theantibody *** and Methods:In this study,we developed an efficient technology for constructing a universal probe(AuNP-PrA-mAb)based on the oriented immobilization of *** performance of ICA was largely improved due to specific binding of the Fc region of the antibody with recombinant protein A(PrA)on the surface of a gold nanoparticle(AuNP).The ICA based on a universal probe was applied for the qualitative and quantitative detection of lead ions in Procambarus clarki within 30 ***,a simple and fast pretreatment method based on dilute acid extraction was developed forpretreating thePclarkii containing ***:The visual limit of detection and the scanning limit of quantization of the developed iCA strip for lead ions were O.5 ng/mL and 0.28 ng/mL,*** sensitivity of ICA based on universal probe was 10-fold higher than that of the ICA using traditional ***,the detection results had no obvious difference between the ICA and ICP-MS with t-test statistical ***:The developed ICA based on a universal probe presented broad application prospects in detecting contaminants in foods.

关键词： Oriented antibody universal probe immunochromatographic assay dilute acid extraction lead ions

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