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Quantitative evaluation of DNA damage caused by atmospheric and room-temperature plasma (ARTP) and other mutagenesis methods using a rapid umu-microplate test protocol for microbial mutation breeding

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Chinese Journal of Chemical Engineering 2021年第11期34卷 205-210页

作者： Yuting Huang Liyang Wang Xue Zhang Nan Su Heping Li Yoshimitsu Oda Xinhui Xing Key Laboratory for Industrial Biocatalysis Ministry of Education of ChinaDepartment of Chemical EngineeringTsinghua UniversityBeijing 100084China Biobreeding Research Center Wuxi Research Institute of Applied TechnologiesTsinghua UniversityWuxi 214072China Department of Entomology College of Plant ProtectionChina Agricultural UniversityBeijing 100193China Department of Engineering Physics Tsinghua UniversityBeijing 100084China Institute of Life and Environmental Sciences Osaka Shin-Ai CollegeTsurumiTsurumi-kuOsaka 5380052Japan Centre for Synthetic and Systems Biology Tsinghua UniversityBeijing 100084China Institute of Biopharmaceutical and Health Engineering Tsinghua Shenzhen International Graduate SchoolShenzhen 518055China Institute of Biomedical Health Technology and Engineering Shenzhen Bay LaboratoryShenzhen 518055China

Mutagenesis is an important technique for microbial mutation *** the source of mutations,DNA damage extent is a key indicator for the effectiveness of ***,a rapid and easy DNA damage quantification method is required for the comparison of mutagenesis effects and development of mutagenesis ***,we used the umu-microplate test system to quantitatively compare the DNA damage strength caused by atmospheric and room-temperature plasma(ARTP)and other traditional mutagenesis methods including:ultraviolet radiation(UV),diethyl sulfate(DES)and 4-nitroquinoline-1-oxide(4-NQO).The test strain of Salmonella typhimurium TA1535/pSK1002 was used to monitor the time-course profile of b-galactosidase activity induced by DNA damage caused by different mutagenesis methods using a microplate *** umu-microplate test results showed that ARTP caused higher extent of DNA damage than UV and chemical mutagens,which agrees well with the result obtained by SOS-FACS-based quantification method as reported *** umu-microplate test is accessible for broad researchers who are lack of the expensive FACS instruments and allows the quick quantitative evaluation of DNA damage among living cells for different mutagenesis methods in the study of the microbial mutation breeding.

关键词： ARTP mutagenesis umu-microplate test Biological engineering Cell engineering Biotechnology

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