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The Herbicidal Activity of Mutant Isolates from Botrytis cinerea

Agricultural Sciences in China 2006年第8期5卷 622-628页

作者： ZHANG Jin-lin ZHANG Li-hui LIU Ying-chao MA Juan LI Chuan DONG Jin-gao Mycotoxin Laboratory Hebei Agricultural University Baoding 071001 P.R.China

Fifteen mutant isolates were obtained by ultraviolet mutation from parent isolate Botrytis cinerea BC-4. Among them three mutant isolates, BC4-1, BC4-2, and BC4-15, showed strong herbicidal activity. BC4-1 showed maximum herbicidal activity for inhibition of germination and growth of Digitaria sanguinalis L. and Arnaranthus retroflexus L. The results also showed that herbicidal activity was influenced by differing pH of PD media, with pH value of 4.0 being the optimum. The crude toxin was extracted using chloroform, petroleum ether, and ethyl acetate, respectively, and the ethyl acetate extracts showed the strongest inhibitory activity on the germination and growth of D. sanguinalis L. and A. retroflexus L. Using HPLC, one fraction with an absorption peak at 271 nm was separated from the crude toxin. This fraction could strongly inhibit the growth of D. sanguinalis L. at a concentration of 100 mg L^-1 and could completely inhibit the seed germination of D. sanguinalis L. and A. retroflexus L. at a concentration of 50 mg L^-1 .

关键词： Botrytis cinerea toxin ultraviolet mutation herbicide activity separation and purification

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ultraviolet Mutagenesis of Bacillus licheniformis LCB-8 and Screening of High-Yielding Strain

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Animal Husbandry and Feed Science 2011年第6期3卷 19-20,22页

作者： LV Zhi-wei WANG Jin ZHANG Wen-hui School of Life Sciences Liaocheng University Liaocheng 252059 China School of Architecture & Civil Engineering Liaocheng Universi-ty Liaocheng 252059 China

[ Objective ] To enhance amylase, protease and cellulase activities of Bacillus licheniformis LCB-8 by ultraviolet mutagenasis. [ Method ] ultraviolet light was used to mutate this strain. The activities of amylase, protease and cellulase were determined to get high-yielding strains. [ Resalt] The best time for ultraviolet mutation was 30 s, and the mortality rate reached 72.4%. After primary screening and rascraening, two better strains were obtained. After mutagenasis, the activities of amylase, protease and cellulase of DY-97 strain were enhanced by 150%, 78% and 17% respectively. After mutagenesis, the activities of cellulase, amylase and protease of DY-34 strain were enhanced by 31%, 46% and 64% respectively. [ Conclusion] The DY-97 strain is suitable for fermentation of feed rich in protein and amylum, whereas the DY-34 strain is suitable for fermentation of feed rich in cellulose.

关键词： Bacillus Iichenfformis ultraviolet mutation Enzyme activity

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