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ultramicrostructure and clinical implications of satellite foci in front of the head of pterygium

Frontiers of Medicine 2007年第2期1卷 211-214页

作者： LIU Haixia XIANG Nan ZHOU Xiongwu HU Weikun LI Guigang ZHANG Hong The Department of Ophthalmology Tongji HospitalHuazhong University of Science and TechnologyWuhan 430030China

In our previous studies,grey satellite foci were found in the front of heads of *** research was designed to investigate the ultramicrostructure and clinical implications of these satellite *** satellite foci were observed and counted under slit lamp *** patients with eye pterygia were divided into groups in terms of occupation,sex,age,length of history,grade of congestion,and size of the *** SPSS 13.0 software was used for statistical *** cap areas and satellite foci were ultramicrostructurally *** the total 62 eyes with pterygium,satellite foci were found in *** overall incidence of satellite foci was 54.8%.There were no significant differences in incidence among the subjects of different sex,age,and length of *** were significant differences in incidence among the patients of different occupation,grades of congestion,and size of *** grades of congestion,outdoor occupations and larger pterygium heads were associated with higher incidence of satellite *** grades of congestion and bigger heads were also correlated with the number of satellite *** of history bore no correlation with number of satellite ***,the components of the cap areas and the foci were identical,with both consisting of mass of active *** activated fibroblasts existed in the natural tissue planes between Bowman’s layer and basal cell *** fibroblasts in the satellite foci and the cap areas of a pterygium show some features of tumor cells and may play a vital role in the development and progression of a *** presence and amount of satellite foci around a pterygium can be used as an indicator for the speed of its growth.

关键词： pterygium ultramicrostructure fibroblast

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Effects of dizocipine maleate on mitochondrial ultramicrostructure in neurons following traumatic brain injury in neonatal rats A quantitative time-course analysis

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Neural Regeneration Research 2008年第2期3卷 208-211页

作者： Huiying Zhang Jun Gu Wenlong Ding Ping Zhu Nursing School of Shanghai Jiao Tong University Shanghai 200025 China Department of Anatomy Medical College of Shanghai Jiao Tong University Shanghai 200025 China Department of Cellbiology Medical College of Shanghai Jiao Tong University Shanghai 200025 China

BACKGROUND： The effects of N-methyl-D-aspartic acid （NMDA） receptor antagonist on neurodegeneration in the immature brain following traumatic brain injury （TBI） are still widely unknown. OBJECTIVE： To study the effects of dizocipine maleate （MK-801）, a non-competitive NMDA receptor antagonist, on mitochondrial ultramicrostructure of neurons in the ipsilateral cingulate cortex and hippocampus after TBI in neonatal rats, and to analyze the optimal time interval of MK-801 administration （1 mg/kg）. DESIGN： Completely randomized controlled study. SETTING： Shanghai Jiao Tong University. MATERIALS： Eight 7-day-old neonatal SD rats, irrespective of gender, were provided by Experimental Animal Center, Medical College of Fudan University. The experiment was approved by a local ethics committee. MK-801 was provided by Sigma. A CM-120 transmission electron microscope （Philips, Holland） was used for tissue analysis. METHODS： This study was performed at the Departments of Anatomy, Neuromorphology, and Biophysics, Medical College of Shanghai, Jiaotong University, between October 2006 and January 2007. Focal models of contusion and laceration of brain were established by the free-falling impact method. Eight rats were randomly divided into a normal control group （n = 2 ） and a MK-801 group （n = 6）. Rats in the normal control group did not receive model establishment and administration, and they were only analyzed by an electron microscope. In the MK-801 group, the cingulate cortex was damaged using a contusion device. MK-801 （1 mg/kg） was intraperitoneally injected 30 minutes before lesion, immediately after lesion, and 30 minutes after lesion （n = 2 for each time point）.MAIN OUTCOME MEASURES： The cingulate cortex and hippocampal tissues from the injured side were removed 24 hours after lesion and routinely processed for analysis of neuronal ultramicrostructure using transmission electron microscopy. RESULTS： Differential therapeutic effects of MK-801 （1 mg/kg） at distinct

关键词： traumatic brain injury neurodegeneration MK-801 ultramicrostructure neonatal rat

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Possible functions of the microtrichia on the cuticle of Ulomoides dermestoides(Chevrolat)(Coleoptera:Tenebrionidae)

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Journal of Forestry Research 2016年第6期27卷 1391-1405页

作者： Jingjing Qian Defu Chi Rusong Chai Northeast Forestry University No.26 at Hexing RoadHarbin 150040Heilongjiang ProvinceChina Heilongjiang Forest Botanical Garden No.105 Haping RoadHarbin 150040Heilongjiang ProvinceChina

Ulomoides dermestoides （Chevrolat）（Coleop- tera： Tenebrionidae） is one of the most notorious pests in northeastern China. We examined microtrichia on the thorax, elytra and abdomen of U. dermestoides using scanning electron microscopy and recorded their confor- mations （size, shape and insertion method） and distribu- tions （length, width and location）. Possible functions of the microtrichia were （1） stridulation： microtrichia on the inner surface of the elytra interacted with microtrichia on the dorsoventral axis of the thorax or on the costal vein of the hind wing; （2） to increase friction： at the major surface on the middle of the abdominal tergum, hind-wings, inner surface of the medial edge of the elytra and the posterior end of the elytra; （3） protection： the microtrichia covering the posterior face of the abdomen conserved water in the body and protected the body from damage; and （4） sensing organ： the special shape of the microtrichia on the nerva- tion near the vannal fold of the hind wing, the anterio- metapleuron on the metathorax, and the posterior field of the abdomen could perceive the environment. In conclusion, the size and shape of the microtrichia are tightly related to their functions, which may have evolved with the beetles＇ lifestyle.

关键词： Coleoptera Cuticle Microtrichia SEM ultramicrostructure

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Breast secretory carcinoma: a clinicopathologic study of four cases

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The Chinese-German Journal of Clinical Oncology 2009年第6期8卷 329-331页

作者： Yihua Chen Juan Li Yi Jian Yanyan Fan Department of Pathology General Hospital of Chengdu Military Command Chengdu 610083 China Department of Pathology Chengdu Medical College Chengdu 610083 China

Objective: To explore the clinicopathologic features of secretory carcinoma of breast (SCB). Methods: Four cases of SCB were analyzed by light microscopy, histochemistry, immunohistochemistry and electron microscopy. The clinical data were also analyzed. Special staining of periodic acid-Schiff reaction with diastase pretreatment, immunohistochemical staining of ER, PR, HER-2, p53, MIB-1, S-100, p63, CK8/18 and EMA by En Vision method were performed. Results: Solid, cribriform, tubular, or papillary architecture may be seen. Tumor forms cystic spaces filled with abundant pale pink secretory material, positive with diastase resistant periodic acid-Schiff (PAS-DR) stains. Tumor cells were small with bland nuclei and abundant pale, eosinophilic cytoplasm, rare mitotic activity and necrosis. Immunohistochemically, tumor cells were positive for CK8/18, EMA, negative for S-100, p63 and variable partially positive for p53, ER, PR, HER-2 and MIB-1. Under electron microscopy, the tumor cells possessed numerous membrane-bound secretory vacuoles in cytoplasm lined by microvilli. Conclusion: SCB is a rare, low-malignant neoplasm. SCB have pathology, clinical picture, treatment, follow-up, immunohistochemical and genetic features that distinguish them from invasive ductal carcinomas of the usual type.

关键词： breast neoplasms secretory carcinoma histochemistry immunohistochemistry ultramicrostructure

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