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ubiquitin-like modifier activating enzyme 2 promotes cell migration and invasion through Wnt/β-catenin signaling in gastric cancer

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World Journal of Gastroenterology 2018年第42期24卷 4773-4786页

作者： Ji Li Xun Sun Ping He Wan-Qi Liu Ya-Bin Zou Quan Wang Xiang-Wei Meng Department of Gastroenterology The First Hospital of Jilin University Department of Pathology The First Hospital of Jilin University Gastrointestinal Surgery The First Hospital of Jilin University

AIM To investigate the function and mechanism of ubiquitinlike modifier activating enzyme 2(Uba2) in progression of gastric cancer(GC) *** Uba2 level in patients with GC was analyzed by Western blotting and immunohistochemistry. MTT and colony formation assays were performed to examine cell *** cytometry was used for cell cycle *** healing and Transwell assays were conducted to examine the effects of Uba2 on migration and *** levels of cell cycle-related proteins, epithelial-mesenchymal transition(EMT) biomarkers, and involvement of the Wnt/β-catenin pathway was assessed by Western blotting. Activation of the Wnt/β-catenin pathway was confirmed by luciferase *** Uba2 expression was higher in GC than in normal *** Uba2 expression was correlated with tissue differentiation, Lauren's classification, vascular invasion,and TNM stage, as determined by the analysis of 100 GC cases(P < 0.05). Knock-down of Uba2 inhibited GC cell proliferation, induced cell cycle arrest, and altered expression of cyclin D1, P21, P27, and Bcl-2, while upregulation of Uba2 showed the opposite effects. The wound healing and Transwell assays showed that Uba2 promoted GC cell migration and invasion. Western blotting revealed alterations in EMT biomarkers, suggesting the role of Uba2 in EMT. Furthermore, the luciferase reporter assay indicated the involvement of the Wnt/β-catenin signaling pathway as a possible modulator of Uba2 oncogenic *** Uba2 plays a vital role in GC cell migration and invasion,possibly by regulating the Wnt/β-catenin signaling pathway and EMT.

关键词： ubiquitin-like modifier activating enzyme 2 Gastric cancer Epithelial-mesenchymal transition Wnt/β-catenin signaling pathway

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OsSCE1 Encoding SUMO E2-Conjugating Enzyme Involves in Drought Stress Response of Oryza sativa

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Rice science 2018年第2期25卷 73-81页

作者： Dini NURDIANI Dwi WIDYAJAYANTIE Satya NUGROHO Laboratory of Genomics and Crop Improvement Research Center for Biotechnology Indonesian Institute of Sciences

Small ubiquitin-like modifier(SUMO)-conjugating enzymes are involved in post-translational regulatory processes in eukaryotes, including the conjugation of SUMO peptides to protein substrate(SUMOylation). SUMOylation plays an important role in improving plant tolerance to abiotic stress such as salt, drought, heat and cold. Herein, we reported the isolation of OsSCE1(LOC_Os10 g39120) gene encoding a SUMO-conjugating enzyme from rice(Oryza sativa cv. Nipponbare) and its functional validation in response to drought stress. The E2 enzyme, Os SCE1, is one of three key enzymes involved in the conjugation of SUMO to its target proteins. Activated SUMO is transferred to the cysteine of an E2 enzyme and then to the target lysine residue of the substrate, with or without the help of an E3 SUMO ligase. Expression of OsSCE1 was strongly induced by polyethylene glycol 6000(PEG6000) treatment, which suggested OsSCE1 may be involved in the drought stress response. Overexpression of OsSCE1(OsSCE1-OX) in Nipponbare reduced the tolerance to drought stress. Conversely, the drought tolerance was slightly improved by the knockdown of OsSCE1(OsSCE1-KD). These results were further supported by measurement of proline content in OsSCE1-OX and OsSCE1-KD transgenic lines under induced drought stress, which showed OsSCE1-KD transgenic lines accumulated higher proline content than the wild type, whereas OsSCE1-OX line had lower proline content than the wild type. These findings suggested OsSCE1 may play a role as a negative regulator in response to drought stress in rice.

关键词： Oryza sativa drought stress small ubiquitin-like modifier SUMO-conjugating enzyme proline content gene expression

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Clinicopathological significance of human leukocyte antigen F-associated transcript 10 expression in colorectal cancer

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World Journal of Gastrointestinal Oncology 2019年第1期11卷 9-16页

作者： Chun-Yang Zhang Jie Sun Xing Wang Cui-Fang Wang Xian-Dong Zeng Department of Emergency Medicine Central Hospital Affiliated to Shenyang Medical College Department of Pathology Central Hospital Affiliated to Shenyang Medical College Department of Surgical Oncology Central Hospital Affiliated to Shenyang Medical College

BACKGROUND Colorectal cancer(CRC) is a common malignancy of the gastrointestinal tract. The worldwide mortality rate of CRC is about one half of its morbidity. ubiquitin is a key regulatory factor in the cell cycle and widely exists in eukaryotes. Human leukocyte antigen F-associated transcript 10(FAT10), known as diubiquitin, is an 18 kDa protein with 29% and 36% homology with the N and C termini of ubiquitin. The function of FAT10 has not been fully elucidated, and some studies have shown that it plays an important role in various cell *** To examine FAT10 expression and to analyze the relationship between FAT10 expression and the clinicopathological parameters of *** FAT10 expression in 61 cases of CRC and para-cancer colorectal tissues was measured by immunohistochemistry and Western blotting. The relationship between FAT10 expression and clinicopathological parameters of CRC was statistically *** Immunohistochemical analysis showed that the positive rate of FAT10 expression in CRC(63.93%) was significantly higher than that in tumor-adjacent tissues(9.84%, P < 0.05) and normal colorectal mucosal tissue(1.64%, P < 0.05). Western blotting also indicated that FAT10 expression was significantly higher in CRC than in tumor-adjacent tissue(P < 0.05). FAT10 expression was closely associated with clinical stage and lymphatic spread of CRC. FAT10 expression also positively correlated with p53 *** FAT10 expression is highly upregulated in CRC. FAT10 expression is closely associated with clinical stage and lymphatic spread of CRC.

关键词： Colorectal cancer ubiquitin ubiquitin-like proteins Human leukocyte antigen F-associated transcript 10 p53

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Viral manipulation of cellular protein conjugation pathways: The SUMO lesson

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World Journal of Virology 2013年第2期2卷 79-90页

作者： Domenico Mattoscio Chiara V Segré Susanna Chiocca Department of Experimental Oncology European Institute of Oncology Via Adamello 16 20139 Milan Italy

Small ubiquitin-like modifier(SUMO)ylation is a key posttranslational modification mechanism that controls the function of a plethora of proteins and biological processes. Given its central regulatory role, it is not surprising that it is widely exploited by viruses. A number of viral proteins are known to modify and/or be modified by the SUMOylation system to exert their function, to create a cellular environment more favorable for virus survival and propagation, and to prevent host antiviral responses. Since the SUMO pathway is a multi-step cascade, viral proteins engage with it at many levels, to advance and favor each stage of a typical infection cycle: replication, viral assembly and immune evasion. Here we review the current knowledge on the interplay between the host SUMO system and viral lifecycle.

关键词： Virus Small ubiquitin-like modifier Small ubiquitin-like modifier-ylation Exploitation Virus assembly Immune evasion Innate immunity

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