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Identification of in vivo induced protein antigens of Salmonella enterica serovar typhi during human infection

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Science China(Life Sciences) 2009年第10期52卷 942-948页

作者： HU Yong1,2, CONG YanGuang1, LI Shu1, RAO XianCai1, WANG Gang3 & HU FuQuan1 1 Department of Microbiology, Third Military Medical University, Chongqing 400038, China 2 Department of Biotechnology, Chongqing University of Technology, Chongqing 400050, China 3 Department of Clinical Laboratory, The 3rd Hospital of People’s Liberation Army, Baoji 721004, China Department of Microbiology Third Military Medical University Chongqing China Department of Biotechnology Chongqing University of Technology Chongqing China Department of Clinical Laboratory The 3rd Hospital of People’s Liberation Army Baoji China

During infectious disease episodes, pathogens express distinct subsets of virulence factors which allow them to adapt to different environments. Hence, genes that are expressed or upregulated in vivo are implicated in pathogenesis. We used in vivo induced antigen technology (IVIAT) to identify antigens which are expressed during infection with Salmonella enterica serovar typhi. We identified 7 in vivo induced (IVI) antigens, which included BcfD (a fimbrial structural subunit), GrxC (a glutaredoxin 3), SapB (an ABC-type transport system), T3663 (an ABC-type uncharacterized transport system), T3816 (a putative rhodanese-related sulfurtransferase), T1497 (a probable TonB-dependent receptor) and T3689 (unknown function). Of the 7 identified antigens, 5 antigens had no cross-immunoreactivity in adsorbed control sera from healthy subjects. These 5 included BcfD, GrxC, SapB, T3663 and T3689. Antigens identified in this study are potential targets for drug and vaccine development and may be utilized as diagnostic agents.

关键词： Salmonella enterica serovar typhi in vivo induced antigen technology (IVIAT) virulence

Induction of deletion mutation on ompR gene of Salmonella enterica serovar typhi isolates from asymptomatic typhoid carriers to evolve attenuated strains for vaccine development

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Asian Pacific Journal of Tropical Medicine 2014年第12期7卷 933-939页

作者： Senthilkumar B Anbarasu K Senbagam D Rajasekarapandian M Centre for Biotechnology Muthayammal College of Arts & SeienceRasipuram-637408Tamil NaduIndia Department of Marine Biotechnology Bharathidasan UniversityTiruchirappalli-620024.Tamil NaduIndia Department of Biotechnology Virekanandha College of Engineering for WomenTiruchengode-637205.Tamil NaduIndia Department of Zoology Arignar Anna Government Arts CollegeNamakkal-637001Tamil NaduIndia

Objective:To develop allenualed slrains of Salmonella enterica serorar typhi(***) for the candidate vaccine by osmolar ***:*** SS3 and SS5 strains were isolated from asymptomatic typhoid carriers in Mamakkal,Tamil *** strains were grown in LB(Luria Bertani) medium supplemented with various concentration of NaCl(0.1- 0.7M) *** effecl of osmolar stress was determined at molecular level by PCR using MCR 06 and MCR07 primers corresponding to ompR with chromosomal DNA of *** SS3 and SS5 *** by osmolar stress results in deletion mutation of *** slrains was determined by agglutination assays,precipitation *** PAGE analysis and by animal ***:The 799 bp amplified ompR gene product from wild type *** SS3 and SS5 illustrate the presence of virulent ***,there was only a 282 bp amplified product from *** SS3 and SS5 grown in the presence of 0.***.0.6 and 0.7 M *** illustrates the occurrence of deletion mutation in ompR gene al high concentration of ***,both the wildtype and mutant *** outer membrane *** reveals the differences in the expression of *** and ompA *** mice,wild type and mutant strains lethal dose(LD_(50)) were *** mice died within 72 h when both the wild type strains were injected intraperitoneally with 3 log CFU-mL^(-1).When the mice were injected with the mutants in same dosage,no clinical symptoms were observed;whereas the serum antibodv litre was elicited within two weeks indicated that the mutants have the ability to induce protective humoral immune *** results suggest that *** SS3 and SS5 may bo used as good candidate strains for the development of live attenuated vaccine against ***:This study demonstrates that the *** strains were allenualed and could be good vaccine candidates in future.

关键词： Attenuated live vaccine Salmonella enterica serovar typhi Mutants

Enhancing chloramphenicol and trimethoprim in vitro activity by Ocimum sanctum Linn.(Lamiaceae) leaf extract against Salmonella enterica serovar typhi

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Asian Pacific Journal of Tropical Medicine 2012年第3期5卷 220-224页

作者： Shyamapada Mandal Manisha Deb Mandal Nishith Kumar Pal Department of Microbiology Bacteriology and Serology UnitCalcutta School of Tropical MedicineC.R.AvenueKolkata-700073India Department of Physiology and Biophysics KPC Medical College and HospitalJadavpurKolkata-700032India

Objective:To evaluate the antibacterial activity of Ocimum sanctum(***) leaf extract, alone,and in combination with chloramphenicol(C) and trimethoprim(Tm) against Salmonella enterica serovar typhi(***).Methods:The antibacterial activity of ethanolic extract of tulsi, ***,leaf(TLE:500μg) for 23 *** isolates was determined following agar diffusion. The C(30μg) and Tm(5μg) activity alone and in combination with TLE(250μg) was determined by disk *** zone diameter of inhibition(ZDI) for the agents was recorded, and growth inhibitory indices(Glls) were ***:The *** isolates(n=23),which were resistant to both C(ZDI 6 mm) and Tm(ZDI 6 mm),had TLE(500μg) ZDIs 16-24 *** ZDIs of C and Tm were increased up to 15-21 mm and 17-23 mm,respectively,when TLE(250μg) was added to the C and Tm *** Glls ranged 0.789-1.235 and 0.894-1.352,due to combined activity against *** isolates,of C and TLE and Tm and ***:The data suggest that TLE,in combination with C and Tm,had synergistic activity for *** isolates, and hence *** is potential in combating *** drug resistance,as well promising in the development of non-antibiotic drug for *** infection.

关键词： Antibacterial activity Ocimum sanctum Chloramphenicol Trimethoprim Growth inhibitory index Synergy Salmonella enterica serovar typhi

Salmonella typhi infection in a tertiary institution in Nasarawa State,Nigeria

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Asian Pacific Journal of Tropical Medicine 2011年第2期4卷 137-139页

作者： Ishaleku D Sar TT Houmsou RS Microbiology Unit Department of Biological SciencesNasarawa State University KeffiP.M.B 1022 KeffiNigeria Department of Biological Sciences University of MkarMkarP.M.B 017GbokoBenue StateNigeria Applied Zoology Unit Department of Biological SciencesTaraba State UniversityJalingoP.M.B 1167 JalingoTaraba StateNigeria

Objective:To evaluate the prevalence of Sabmonella typhi(***) among subjects attending College of Education Health Clinic,Akwanga,Nasarawa state from the year.2005 to 2007 and to recommend some preventive measures to the ***:Blood samples were tested for infection using the widal ***:Out of the 793 patients examined,579(73.0%) were positive with 174(30.05%),254(43.86%) and 151(26.07%) for the years 2005,2006 and 2007 respectively. The age range with the highest prevalence of infection for the period was 21-30 with 207(35.75%) followed by 31-40 year group with 133(22.97%).Chi-square analysis showed no significant difference in infection between males and females(P>0.05).Conclusions:The results of this study provide a useful guide in the formulation of *** control measures in tertiary institutions in the State and also help to check the spread of the pathogen in the general population.

关键词： Salmonella typhi Infection Tertiary institution Nasarawa State

A cross sectional study on antibiotic resistance pattern of Salmonella typhi clinical isolates from Bangladesh

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Asian Pacific Journal of Tropical Biomedicine 2014年第4期4卷 306-311页

作者： Adnan Mannan Mohammad shohel sultana Rajia Niaz Uddin Mahmud Sanjana Kabir Imtiaj Hasan Department of Genetic Engineering and Biotechnology Faculty of Biological SciencesUniversity of Chittagong Department of Pharmacy North South University Department of Pharmacy Varendra University Department of Computer Science & Engineering BGC Trust University Bangladesh Department of Biochemistry and Molecular Biology Rajshahi University Laboratory of Glycobiology and Marine Biochemistry Department of Life and Environmental System ScienceGraduate School of Nano BiosciencesYokohama City University

Objective:To investigate and compare the resistance and sensitivity of Salmonella typhi samples to commonly used antibiotics in three major divisions of Bangladesh and to evaluate the gradually developing resistance ***:The antibiotic susceptibility of 70 clinical isolates collected from blood,sputum,urine and pus samples were identified by specific antisera and with standard biochemical *** patients were divided into 5 age *** and resistance was also tested by KirbyBauer disc-diffusion method using 12 regularly used ***:Antibiotic susceptibility test demonstrated that 64.28%isolates of Salmonella typhi were multidrug *** study suggests that the clinical samples were mostly resistant against nalidixic acid with all age groups and in all three divisions with similar resistance *** is more common among adult people(30-40 years)and children(0-10 years).Salmonella typhi was mostly sensitive against gentamycin,chloramphenicol and ***:Although the population density of Dhaka region is markedly higher than Rajshahi and Chittagong regions,no significant difference in resistance pattern was *** rate of multidrug resistance is a matter of *** should reconsider before prescribing nalidixic acid and *** molecular study is needed to reveal the genomic and proteomic basis of resistance.

关键词： Bangladesh Salmonella typhi Resistance Susceptibility Antibiotic

Analysis of Trends in Resistance to Fluoroquinolones and Extended Spectrum Beta-Lactams among Salmonellatyphi Isolates Obtained from Patients at Four Outpatient Clinics in Nairobi County, Kenya

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Advances in Microbiology 2018年第7期8卷 578-588页

作者： Susan Mutile Kavai Mourine Kangogo Anne W. T. Muigai Samuel Kariuki Institute of Tropical Medicine and Infectious Diseases Jomo Kenyatta University of Agriculture and Technology Nairobi Kenya Department of Medical Microbiology Jomo Kenyatta University of Agriculture and Technology Nairobi Kenya Department of Botany Jomo Kenyatta University of Agriculture and Technology Nairobi Kenya Centre for Microbiology Research Kenya Medical Research Institute Nairobi Kenya

Typhoid fever caused by the bacterium Salmonella enterica serovar typhi (S. typhi) causes an estimated 25 million illnesses and approximately 200,000 deaths annually mostly in developing countries. Although the management of typhoid fever has been effectively through antibiotic treatment, S. typhi is increasingly becoming resistant to the currently recommended drugs. This study utilized a quasi-experimental design focusing on archived samples to describe antimicrobial susceptibility patterns of S. typhi and determine the genetic basis of resistance to the two most commonly used classes of antimicrobials. A total sample size of 287 isolates of S. typhi isolates stored in -80°C freezer at the Centre for Microbiology Research was utilized. Isolates were subjected to anti-microbial susceptibility testing to commonly available antimicrobials using disk diffusion method, then analyzed for trends in resistance to fluoroquinolones and extended spectrum beta lactams. Among the 287 isolates 158 (55.5%) were found to be Multi Drug Resistant (MDR). This implied that these isolates were resistant to all first line classes of treatment such as ampicillin, chloramphenicol and sulfamethoxazole-trimethroprim. In addition to this, these isolates were also resistant to at least one of the currently recommended drugs of choice, either a β-lactam or a fluoroquinolone. This study observed resistances at 18.2% and 15.4% to fluoroquinolones and cephalosporins respectively. PCR results revealed presence of blaTEM, blaINT and blaCTX-M genes coding for resistance to β-lactams in 80% of the isolates that had combined resistance to β-lactams and fluoroquinolones. It is likely that recent heavy use of these classes of antimicrobials is driving resistances to these antimicrobials.

关键词： Resistance Trends Salmonella typhi β-Lactams Flouroquinolones Nairobi County Kenya

Comparative Study on Bactericidal Effect of Silver Nanoparticles, Synthesized Using Green Technology, in Combination with Antibiotics on Salmonella typhi

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Journal of Biomaterials and Nanobiotechnology 2012年第4期3卷 480-485页

作者： Shweta Rajawat Mohammad Shums Qureshi Department of Physics Maulana Azad National Institute of Technology Bhopal India

In this work bactericidal study of silver nanoparticles was taken up in combination with two standard antibiotics, ampicillin and gentamycin, for Salmonella typhi. The antibacterial activities of antibiotics were increased in the presence of AgNPs against test strains. The higher enhancing effect was observed for ampicillin in comparison to gentamicin against test strains. Silver nanoparticles were synthesized elctrolytically using silver wire of 99% purity as anode and carbon rod wrapped with LDPE as cathode. Silver nitrate [of Merck] of 0.01N is used as an electrolyte. Here tea extract is added as capping and mild reducing agent. The polyphenols theaflavins and thearubigins, present in tea perform the role of stabilizing or capping agents due to their bulky and steric nature. A brown coloured colloidal solution of silver nanoparticles is obtained. The as-synthesized silver nanoparticles were characterized using XRD, TEM and UV-Vis spectroscopy.

关键词： Silver Nanoparticles Salmonella typhi Tea Extract Ampicillin Gentamicin

Effect of Chromolaena odorataExtracton Hematotoxicity and Spleen Histopathology Induced by Salmonella typhiin Wistar Rats

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Pharmacology & Pharmacy 2018年第4期9卷 85-99页

作者： Joshua Charles Isirima Iyeopu Minakiri Siminialayi Department of Biomedical Technology University of Port Harcourt Port Harcourt Nigeria Department of Pharmacology University of Port Harcourt Port Harcourt Nigeria

Salmonella typhi is a facultative intracellular pathogen that causes typhoid fever in humans. In the present study, the effect of Salmonella typhi infection on hematological indices and spleen histology in Wistar rats was investigated and was followed by an evaluation of the ameliorative potential of the methanol leaf extract of Chromolaena odorata (MLECO) compared with ciprofloxacin treatment. The animals were divided into six groups: group 1 was normal control, group 2 was infected with Salmonella typhi without treatment (negative control), groups 3, 4 and 5 were Salmonella typhi infected and treated with 100 mg/kg, 200 mg/kg and 400 mg/kg of the extract respectively and group 6 was also Salmonella typhi infected and treated with 500 mg/70kg of ciprofloxacin. The animals were inoculated with a single infectious dose of Salmonella typhi bacteria and thereafter, treated with the extract and ciprofloxacin for a period of seventeen days, after the animals were confirmed infected. The rats were humanely sacrificed and blood samples taken for haematological investigations, and the spleen harvested and processed for histological examinations. Chromolaena odorata administration reversed the adverse hematotoxicity and histopathological changes in the spleen induced by?Salmonella typhi?infection.

关键词： Chromolaena odorata Hematotoxicity Spleen Histopathology Salmonella typhi Wistar Rats

CONSTRUCTION OF A BIVALENT ORAL VACCINE FOR PREVENTION OF TYPHOID FEVER AND CHOLERA DIARRHEA

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Science China Chemistry 1990年第1期33卷 44-49页

作者：马清钧于秀琴刘传暄周建光熊凌霜黄翠芬 Institute of Biotechnology Academy of Military Medical Sciences Beijing 100850 PRC

A recombinant plasmid pMM-CTB containing the gene for production of the nontoxic B subunit of Vibrio cholera was transferred into a safe, effective and attenuated oral vaccine Ty21a strain of Salmonella typhi. The resulting Ty21a(pMM-CTB) could steadily produce CT-B subunit that was secreted extracellularly and had the same antigenicity as CT-B produced by V. cholera. Furthermore, the characteristics of the antigenicity, the persistance in mice and the galactose sensitivity possessed in the strain of Ty21a were also retained in Ty21a (pMM-CTB). A bivalent vaccine containing Ty21a (pMM-CTB) and the killed whole cell of V. cholera was then constructed which had good immunogenecity for typhoid fever and cholera diarrhea.

关键词： oral cholera vaccine S. typhi vaccine.