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Transcriptome analysis on dominant GMS homozygous two-type line D3AB in Brassica napus L. by RNA-Seq

Transcriptome analysis on dominant GMS homozygous two-type l...

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中国作物学会油料作物专业委员会第八次会员代表大会暨学术年会

作者： JIA Le-dong XU Dai-xiang MA Guo-qiang DUAN Mou-zheng QIAO Cai-lin ZHOU Xin-tong QU Cun-min WANG Rui LI Jia-na Chongqing Rapeseed Engineering Technology Research Center College of Agronomy and BiotechnologySouthwest University Academy of Agricultural Sciences Southwest University

Genie male sterility(GMS) has been widely used in Brassica napus breeding because of its stable and thorough sterility and wide distribution of restorer lines.D3 AB is a near isogenic line(NIL) controlled by a single dominant gene,but the specific molecular mechanisms leading to sterility is still *** this study,the transcriptome data of male sterile D3 A and fertile D3 B at different developmental stages of buds was compared,and some related genes causing abortion were identified.A total of 2 210 differentially expressed genes(DEGs) were detected,of which 374 genes were differentially expressed between young buds(≤1 mm) and middle buds(1-2 mm) in D3 A,and 1 458 in D3 B ***,679 differentially expressed genes were detected in young buds of D3 A and D3 B,and 1 423 in the middle buds ***,based on the results of cytological analysis,90 DEGs were specifically expressed in young buds,23 genes of them were up-regulated and67 were down-regulated,*** the gene expression profile database of Arabidopsis thaliana,10 DEGs were specifically expressed in young flower buds or pollen grains,and the expression patterns of 9 DEGs were consistent with transcriptome sequencing by *** results lay the foundation for further study on the abortive mechanism of D3 AB in Brassica napus.

关键词： Brassica napus dominant GMS two-type line RNA-seq

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