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Activation and signaling of the p38 MAP kinase pathway

Cell Research 2005年第1期15卷 11-18页

作者： Tyler ZARUBIN La Jolla USA Department of Immunology 10550 N. Torrey Pines Road The Scripps Research Institute CA 92037

The family members of the mitogen-activated protein (MAP) kinases mediate a wide variety of cellular behaviors in response to extracellular stimuli. One of the four main sub-groups, the p38 group of MAP kinases, serve as a nexus for signal transduction and play a vital role in numerous biological processes. In this review, we highlight the known characteristics and components of the p38 pathway along with the mechanism and consequences of p38 activation. We focus on the role of p38 as a signal transduction mediator and examine the evidence linking p38 to inflammation, cell cycle, cell death, development, cell differentiation, senescence and tumorigenesis.in specific cell types. Upstream and downstream components of p38 are described and questions remaining to be answered are posed. Finally, we propose several directions for future research on p38.

关键词： p38 MAP kinase signaling pathway nexus inflammation differentiation senescence tumorigenesis.

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A novel human gene spindlin1, encoding a protein localized in the cell nucleus and inducing NIH3T3 cell's transformation

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Progress in Natural Science:Materials International 2004年第11期 30-34+98页

作者： GAO Yanhong, QIN Lipeng, ZHANG Peng, CHEN Lin, YUAN Hongfeng, BAI Cixian, YAN Fang, YUE Wen* and PEI Xuetao*(Department of Stem Cell Biology, Beijng Institute of Transfusion Medicine, Beijing 100850, China) DepartmentofStemCellBiology BeijngInstituteofTransfusionMedicineBeijing100850China

A novel human gene, spindlin1, recently cloned in our laboratory, is highly expressed in the tissue of ovary cancer. To study its biological function, a vector expressing green fluorescent-spindlin1 fusion protein was constructed and transfected into COS-7 and NIH3T3 cells by lipofectamine methods. The results showed that the fusion protein pEGFP-N1-spindlin1 was localized in the nucleus of COS-7 and NIH3T3 cells. NIH3T3 cells which could stably express spindlin1 as a result of RT-PCR analysis compared with the parental NIH3T3 cells displayed a complete morphological change, improved the cell growth and increased the percentage of cells in G 2/M phase (12.6% vs control cells at 3.4%). Furthermore, overexpressed spindlin1 cells formed colonies in soft agar, more motile in migration assay in vitro and formed tumors in nude mice. Our findings provide direct evidence that spindlin1 gene may be a prooncogene which is associated with tumorigenesis.

关键词： spindlin1 gene transfection subcellular localization tumorigenesis.

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