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Selective tropism of liver stem cells to hepatocellular carcinoma in vivo

World Journal of Gastroenterology 2007年第28期13卷 3886-3891页

作者： Xiao-Gang Zhong Sheng He Wu Yin Jing-Yu Deng Bo Cheng Department of General Surgery The People's Hospital of Guangxi Zhuang Autonomous Region Nanning 530021 Guangxi Zhuang Autonomous Region China Department of General Surgery West China Hospital Sichuan University Chengdu 610041 Sichuan Province China Department of Pathology West China Hospital Sichuan University Chengdu 610041 Sichuan Province China

AIM： To investigate the selective tropism of liver stem cells to hepatocellular carcinoma （HCC） in an animal model and its feasibility as a vector to deliver therapeutic genes for targeted therapy of HCC.METHODS： WB-F344, a kind of rat liver stem cell, was infected with recombinant virus to establish a cell line with stable, high-level expressing enhanced green fluorescent protein （EGFP）. An animal model of HCC in Wistar rats was established by implanting HCC cells （CBRH7919） combined with an immunosuppressive drug. EGFP labeled liver stern cells were injected into caudal veins of the animals and distribution was observed at different time points after injection. SDF-1 and c-kit expression in non-tumor liver and tumor tissue were analysed by immunohistochemistry for the relationshiop between the expression and migration of liver stem cells. Furthermore, hepatic stern cells were injected via the portal vein, hepatic artery, caudal vein, or directly into the pericancerous liver tissue, respectively, and effects on migration, localization, and proliferation of the hepatic stern cells within the tumor tissue were observed and analyzed.RESULTS： Recombinant adenovirus could deliver the EGFP gene to hepatic stem cells. A new stem cell line, named WB-EGFP, was established that stably expressed EGFP. WB-EGFP cells still showed selective tropism towards HCC and EGFP expression was stable in vivo. According to immunohistochemistry results, SDF-1 may not be related to the mechanisms of tropism of hepatic stem cells. Different application sites affected the distribution of liver stem cells. Injection via the portal vein was superior with regard to selective migration, localization, and proliferation of the hepatic stem cells within the tumor tissue.CONCLUSION： Liver stem cells have the biological behavior of selective migration to HCC in vivo and they could localize and proliferate within HCC tissue stably expressing the target gene. Liver stem cells are a potential tool f

关键词： Liver Stem cells Hepatocellular carcinoma Vector tropism Gene therapy

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Investigation of the differences between the “COLD” and “HOT” nature of Coptis chinensis Franch and its processed materials based on animal’s temperature tropism

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Science China(Life Sciences) 2009年第11期52卷 1073-1080页

作者： ZHOU CanPing1,2, WANG JiaBo1, ZHANG XueRu1,2, ZHAO YanLing1, XIA XinHua2, ZHAO HaiPing1, REN YongShen1 & XIAO XiaoHe1 1 China Military Institute of Chinese Meteria Medica, 302 Military Hospital of China, Beijing 100039, China 2 College of Pharmaceutical Sciences, Hunan University of Chinese Medicine, Changsha 410208, China China Military Institute of Chinese Meteria Medica 302 Military Hospital of China Beijing China College of Pharmaceutical Sciences Hunan University of Chinese Medicine Changsha China

The description and differentiation of the so-called "Cold" and "Hot" natures, the primary "Drug Naure" of Chinese medicine, is the focus of theoretical research. In this study, the divergency between the "Cold" and the "Hot" natures was investigated through examining the temperature tropism of mice affected by Coptis chinensis Franch and its processed materials by using a cold/hot plate differentiating technology. After exposure to C. chinensis Franch, the macroscopic behavioral index of the remaining rate (RR) on a warm pad (40℃ ) significantly increased (Ptropism. The internal indexes of adenosine triphosphatase (ATPase) activity and oxygen consuming volume decreased significantly (Ptropism might reflect the internal Cold nature of C. chinensis Franch. However, the processed materials of C. chinensis Franch exhibited a different Cold nature in temperature tropism compared with crude C. chinensis Franch (CC): the Cold nature of bile-processed C. chinensis Franch (BC) enhanced while the ginger-processed C. chinensis Franch (GC) changed inversely. The changing sequence was consistent with the theoretical prognostication. It is indicated that the external Cold & Hot natures of Chinese medicine may possibly reflect in an ethological way for the changes of animal’s temperature tropism which might be internally regulated by the body’s energy metabolism.

关键词： "Cold" and "Hot" natures of Chinese medicine evaluation temperature tropism ethology drug process Coptis chinensis Franch

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Different meridian tropism in three Chinese medicines: Tinglizi(Semen Lepidii Apetali), Yiyiren(Semen Coicis), Cheqianzi(Semen Plantaginis)

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Journal of Traditional Chinese Medicine 2019年第2期39卷 213-220页

作者： Zeng Mengnan Li Miao Zhang Li Zhang Beibei Wu Guangcao Feng Weisheng Zheng Xiaoke Department of Pharmacy Henan University of Chinese Medicine Department of Pharmacy Henan University of Chinese Medicine Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment and Chinese Medicine Development of Henan Province

OBJECTIVE: To study the meridian tropism(MT) for three Chinese medicines: Tinglizi(Semen Lepidii Apetali, TLZ), Yiyiren(Semen Coicis, YYR), Cheqianzi(Semen Plantaginis, CQZ) in vivo ***: The extracts of TLZ,YYR, CQZ were orally administered to rats at a dose 234, 700 and 350 mg/kg, respectively. The urinary excretion rate, loss in weight of rats and the electrolyte levels in the plasma were measured, in order to assess the mechanisms of the three traditional Chinese diuretic ***: TLZ, YYR and CQZ exhibited obvious diuretic effect and reduced the weight of saline-loaded animals. All the three diuretic medicines differentially reduced various serum, urinary, lung, stomach, kidney and hormonal markers, thus indicating the different mechanisms of action and a close resemblance with their different meridian as per the MT ***: Our findings suggest that the three Traditional Chinese Medicine(TCM) medicines-TLZ,YYR and CQZ-possess different Shamisen *** can support the regimen of treating the same disease with different therapies in TCM theory.

关键词： Medicine,Chinese traditional Diuretics Meridian tropism

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Tissue and host tropism of influenza viruses:Importance of quantitative analysis

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Science China(Life Sciences) 2009年第12期52卷 1101-1110页

作者： ZHANG Hong1,2 1 Z-BioMed, Inc., Rockville, MD 20855, USA 2 Department of Respiratory Medicine, Affiliated Hospital of Zunyi Medical College, Zunyi 563003, China Z-BioMed Inc. Rockville USA Department of Respiratory Medicine Affiliated Hospital of Zunyi Medical College Zunyi China

It is generally accepted that human influenza viruses preferentially bind to cell-surface glycoproteins/ glycolipids containing sialic acids in α2,6-linkage; while avian and equine influenza viruses preferentially bind to those containing sialic acids in α2,3-linkage. Even though this generalized view is accurate for H3 subtype isolates, it may not be accurate and absolute for all subtypes of influenza A viruses and, therefore, needs to be reevaluated carefully and realistically. Some of the studies published in major scientific journals on the subject of tissue tropism of influenza viruses are inconsistent and caused confusion in the scientific community. One of the reasons for the inconsistency is that most studies were quantitative descriptions of sialic acid receptor distributions based on lectin or influenza virus immunohistochemistry results with limited numbers of stained cells. In addition, recent studies indicate that α2,3- and α2,6-linked sialic acids are not the sole receptors determining tissue and host tropism of influenza viruses. In fact, determinants for tissue and host tropism of human, avian and animal influenza viruses are more complex than what has been generally accepted. Other factors, such as glycan topology, concentration of invading viruses, local density of receptors, lipid raft microdomains, coreceptors or sialic acid-independent receptors, may also be important. To more efficiently control the global spread of pandemic influenza such as the current circulating influenza A H1N1, it is crucial to clarify the determinants for tissue and host tropism of influenza viruses through quantitative analysis of experimental results. In this review, I will comment on some conflicting issues related to tissue and host tropism of influenza viruses, discuss the importance of quantitative analysis of lectin and influenza virus immunohistochemistry results and point out directions for future studies in this area, which should lead to a better understanding

关键词： tissue and host tropism influenza viruses sialic acid receptors quantitative analysis

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Tissue tropism and parasite burden of Toxoplasma gondii RH strain in experimentally infected mice

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Asian Pacific Journal of Tropical Medicine 2014年第7期7卷 521-524页

作者： Yousef Dadimoghaddam Ahmad Daryani Mehdi Sharif Ehsan Ahmadpour Zahra Hossienikhah Toxoplasmosis Research Center Mazandaran University of Medical Sciences Parasitology and Mycology Department.Sari Medical School Mazandaran University of Medical Sciences Molecular and Cell Biology Research Center Mazandaran University of Medical Sciences

Objective:To evaluate parasite distribution and tissue tropism of Toxoplasma gondii tachyzoites in experimentally infected mice using real time ***:In this survey 16 Balb/c mice were inoculated with 1×10~4 alive tachyzoites of Toxoplasma gondii RH *** 1,2,3 days post infection and the last day(before death),different tissues of mice including blood,brain,eye,liver,spleen,kidney,heart and muscle were *** tissues DNA extraction,the parasite burden was quantified using real time QPCR targeting the B1 gene(451 bp).Results:It showed that Toxoplasma after intraperitoneal injection was able to movement to various tissues in24 *** burden was high in all tissues but the most number of parasites were observed in kidney,heart and liver,***:These data provide significant baseline information about Toxoplasma pathogenesis,vaccine monitoring and drug efficiency.

关键词： Parasite burden Toxoplasma gondii Tissue tropism Quantitative PCR Murine model

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Hot Property of Aconiti Lateralis Radix Praeparata and its Compatibility with Zingiberis Rhizoma Based on Animal Temperature tropism

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Chinese Herbal Medicines 2012年第4期4卷 -页

作者： SUN Zhi-yong1 1. Integrative Medicine Center of 302 Military Hospital China Military Institute of Chinese Materia Medica Beijing 100039 China 2. Chengdu University of Traditional Chinese Medicine Chengdu 610075 China 3. Animal Laboratory Center 302 Hospital of PLA China

Abstract: Objective To establish an objective method for evaluating the intrinsic characteristics between hot and cold properties of herbal drugs and to study the hot property of Aconiti Lateralis Radix Praeparata (ALRP) and its compatibility with Zingiberis Rhizoma (ZR) on animal temperature tropism. Methods The equipment with cold/hot pads was used to investigate the variety of temperature tropism among mice treated by ALRP and its compatibility with ZR. Meanwhile, the activities of adenosine triphosphatase (ATPase), total anti-oxidant capability (T-AOC), and total superoxide dismutase (T-SOD) were measured. Results Compared with the homologous ALRP group, the compatibility between ALRP and ZR (AZ) had a stronger action against the decreasing of remaining rate on hot pad (P tropism. Meanwhile, the internal indexes of Na+-K+-ATPase activity, Ca2+-Mg2+-ATPase activity, and oxygen consuming volume increased significantly (P tropism and the internal activity of energy metabolism and anti-oxidant might reflect the internal hot property in an intuitive and objective way. The changes of ATPase activity of liver tissue might be the mechanism of drug action.

关键词： Key words: Aconiti Lateralis Radix Praeparata animal temperature tropism clustering analysis compatibility Zingiberis Rhizoma

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Facet orientation and tropism:Associations with asymmetric lumbar paraspinal and psoas muscle parameters in patients with chronic low back pain

Facet orientation and tropism:Associations with asymmetric l...

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2016年浙江省骨科学学术年会

作者： Xu Wenbin chen shuai fan shunwu zhao fengdong yu xiaojing hu zhijun Sir Run Run Shaw Hospital

OBJECTIVE:To analyze the associations between facet orientation and tropism,and parameters of paraspinal muscles in patients with chronic low back ***:Ninety-five patients with chronic low back pain were consecutively *** facet joint angles were measured on computed tomography(CT) while gross cross-sectional area(GCSA),functional cross-sectional area(FCSA) and T2 signal intensity of lumbar paraspinal and psoas muscle were evaluated on magnetic resonance imaging(MRI).RESULTS:The GCSA and FCSA were significantly smaller for multifidus muscle(Ptropism group than those in no facet tropism group(P= 0.009 and P= 0.019).CONCLUSIONS:Muscular asymmetries may develop in the lumbar region of the spine,which are associated with facet asymmetry in patients with chronic low back *** studies are needed to understand the causal relationship between facet orientation and tropism and muscular asymmetry in future.

关键词： Facet orientation multifidus tropism cross-sectional area magnetic resonance imaging

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Stem cells tropism for malignant gliomas

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Neuroscience Bulletin 2007年第6期23卷 363-369页

作者：徐锋朱剑虹复旦大学附属华山医院神经外科上海市神经外科临床医学中心上海200040

Various studies have demonstrated the tremendous tropism of stem cells for malignant gliomas,making these cells a potential vehicle for delivery of therapeutic genes to disseminated glioma ***,little is known about the mechanisms underlying the glioma-induced tropism of stem *** factors including chemokines or growth factors released and expressed by glioma cells at least mediate the tropism of stem cells for *** we review the possible mechanisms of stem cells tropism for malignant gliomas.

关键词： stem cell tropism glioma

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Active constituents and mechanisms of Respiratory Detox Shot, a traditional Chinese medicine prescription, for COVID-19 control and prevention: Network-molecular docking-LC–MS^E analysis

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Journal of Integrative Medicine 2020年第3期18卷 229-241页

作者： Zi-jia Zhang Wen-yong Wu Jin-jun Hou Lin-lin Zhang Fei-fei Li Lei Gao Xing-dong Wu Jing-ying Shi Rong Zhang Hua-li Long Min Lei Wan-ying Wu De-an Guo Kai-xian Chen Lewis A.Hofmann Zhong-hua Ci Shanghai Institute of Materia Medica Chinese Academy of SciencesShanghai 201203China University of Chinese Academy of Sciences Beijing 100049China World Health Science Organization LeesburgVA 20176USA

Objective: Lung-toxin Dispelling Formula No. 1, referred to as Respiratory Detox Shot(RDS), was developed based on a classical prescription of traditional Chinese medicine(TCM) and the theoretical understanding of herbal properties within TCM. Therapeutic benefits of using RDS for both disease control and prevention, in the effort to contain the coronavirus disease 2019(COVID-19), have been shown. However,the biochemically active constituents of RDS and their mechanisms of action are still unclear. The goal of the present study is to clarify the material foundation and action mechanism of ***: To conduct an analysis of RDS, an integrative analytical platform was constructed, including target prediction, protein–protein interaction(PPI) network, and cluster analysis;further, the hub genes involved in the disease-related pathways were identified, and the their corresponding compounds were used for in vitro validation of molecular docking predictions. The presence of these validated compounds was also measured in samples of the RDS formula to quantify the abundance of the biochemically active constituents. In our network pharmacological study, a total of 26 bioinformatic programs and databases were used, and six networks, covering the entire Zang-fu viscera, were constructed to comprehensively analyze the intricate connections among the compounds-targets-disease pathways-meridians of ***: For all 1071 known chemical constituents of the nine ingredients in RDS, identified from established TCM databases, 157 passed drug-likeness screening and led to 339 predicted targets in the constituent–target network. Forty-two hub genes with core regulatory effects were extracted from the PPI network, and 134 compounds and 29 crucial disease pathways were implicated in the target–constitu ent–disease network. Twelve disease pathways attributed to the Lung–Large Intestine meridians, with six and five attributed to the Kidney–Urinary Bladder and Stomach–Spleen meridian

关键词： Lung-toxin Dispelling Formula No.1 COVID-19 3C-like protease Network pharmacology Molecular docking Meridian tropism

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Specific VpU Codon Changes were Significantly associated with gp120 V3 Tropic Signatures in HIV-1 B-subtype

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Virologica Sinica 2012年第6期27卷 360-368页

作者： Salvatore Dimonte Muhammed Babakir-Mina Stefano Aquaro Carlo-Federico Perno Department of Experimental Medicine and Surgery University of Rome"Tor Vergata"Rome 00133Italy Department of Experimental Medicine and Surgery University of Rome"Tor Vergata"Rome 00133Italy Slemani Polytechnic University Iraqi Kurdistan Region Faculty of Pharmacy Nutritional and Health SciencesUniversity of CalabriaArcavacata di Rende(CS)87036Italy National Institute for Infectious Diseases"L.Spallanzani" Rome 00149Italy

After infection and integration steps, HIV-1 transcriptions increase sharply and singly-spliced mRNAs are produced. These encode Env （gpl20 and gp41） and auxiliary proteins Vif, Vpr and VpU. The same localization within the unique structure of the mRNAs suggests that the VpU sequence prior to the Env could affect the Env polyprotein *** HIV-I infection process begins when the gpl20 subunit of the envelope glycoprotein complex interacts with its receptor（s） on the target cell. The V3 domain of gpl20 is the major determinant of cellular co-receptor binding. According to phenotypic information of HIVol isolates, sequences from the VpU to V3 regions （119 in R5- and 120 X4-tropic viruses; one per patient） were analysed. The binomial correlation phi coefficient was used to assess covariation among VpU and gpl20v3 signatures. Subsequently, average linkage hierarchical agglomerative clustering was performed. Beyond the classical V3 signatures （R5-viruses： SI1, E25D; X4-viruses： SllKR, E25KRQ）, other specific V3 and novel VpU signatures were found to be statistically associated with co-receptor usage. Several statistically significant associations between V3 and VpU mutations were also observed. The dendrogram showed two distinct large clusters： one associated with R5-tropic sequences （bootstrap=0.94）, involving：（a） H13NPv3, E25Dv3, Sllv3, T22Av3 and Q61Hvpu, （b） E25Av3 and L12Fvpu, （c） D44Evpu, R18Qv3 and D80Nvpu; and another associated with X4-tropic sequences （bootstrap=0.97）, involving：（i） E25Iv3 and V10Avpu, （ii） 0-1insVvpc, H13Rv3, I46Lvpc, I30Mv3 and 60-62delvpu, （iii） SllKRv3 and E25KRQv3. Some of these pairs of mutations were encoded always by one specific codon. These data indicate the possible VpU mutational patterns contributing to regulation of HIV-I tropism.

关键词： HIV VpU gpl20 V3 Mutations tropism Cluster analysis

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