检索结果-南通市图书馆

Esrrb plays important roles in maintaining self-renewal of trophoblast stem cells (TSCs) and reprogramming somatic cells to induced TSCs

引用

Journal of Molecular Cell Biology 2019年第6期11卷 463-473页

作者： Haibo Gao Rui Gao Linfeng Zhang Wenchao Xiu Ruge Zang Hong Wang Yong Zhang Jiayu Chen Yawei Gao Shaorong Gao Clinical and Translational Research Center of Shanghai First Maternity and Infant Hospital Shanghai Key Laboratory of Signaling and Disease Research School of Life Sciences and Technology Tongji University Shanghai 200092 China Peking University-Tsinghua University-National Institute of Biological Sciences Joint Graduate Program School of Life Sciences Tsinghua University Beijing 100084 China

trophoblast stem cells (TSCs), which can be derived from the trophoectoderm of a blastocyst, have the ability to sustain self-renewal and differentiate into various placental trophoblast cell types. Meanwhile, essential insights into the molecular mechanisms controlling the placental development can be gained by using TSCs as the cell model. Esrrb is a transcription factor that has been shown to play pivotal roles in both embryonic stem cell (ESC) and TSC, but the precise mechanism whereby Esrrb regulates TSC-specific transcriptome during differentiation and reprogramming is still largely unknown. In the present study, we elucidate the function of Esrrb in self-renewal and differentiation of TSCs, as well as during the induced TSC (iTSC) reprogramming. We demonstrate that the precise level of Esrrb is critical for stem state maintenance and further trophoblast differentiation of TSCs, as ectopically expressed Esrrb can partially block the rapid differentiation of TSCs in the absence of fibroblast growth factor 4. However, Esrrb depletion results in downregulation of certain key TSC-specific transcription factors, consequently causing a rapid differentiation of TSCs and these Esrrb-deficient TSCs lose the ability of hemorrhagic lesion formation in vivo. This function of Esrrb is exerted by directly binding and activating a core set of TSC-specific target genes including Cdx2, Eomes, Sox2, Fgfr4, and Bmp4. Furthermore, we show that Esrrb overexpression can facilitate the MEF-to-iTSC conversion. Moreover, Esrrb can substitute for Eomes to generate GEsTM-iTSCs. Thus, our findings provide a better understanding of the molecular mechanism of Esrrb in maintaining TSC self-renewal and during iTSC reprogramming.

关键词： Esrrb trophoblast stem cell self-renwwal differentiation iTSC reprogramming

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

UCK2 promotes the proliferation,migration,and invasion of trophoblast cells in preeclampsia by activating the STAT3 pathway

引用

BIOCELL 2023年第4期47卷 837-847页

作者： WEI XIA NING YANG XIAOYAN FENG TING XIN YONGLE JING YUMING LI CHENGZHI LU Department of Cardiology Tianjin First Central HospitalTianjin300192China Department of Hypertension TEDA International Cardiovascular HospitalTianjin300457China Department of Dermatology Tianjin Children’s HospitalTianjin300134China TEDA International Cardiovascular Hospital Tianjin300457China School of Medicine Nankai UniversityTianjin300071China

Background:Preeclampsia(PE),characterized by hypertension and proteinuria,leads to serious maternal and infant ***-cytidine kinase 2(UCK2)belongs to the UCK family,a class of enzymes that catalyzes the conversion of uridine and cytidine to monophosphate ***,the role of UCK2 in PE has not been ***:The expression of UCK2 was detected in the placenta of PE patients and N(ω)-nitro-L-arginine methyl esterinduced PE mouse *** forced up-regulation or down-regulation of UCK2 in vitro,we examined the effects of UCK2 on the proliferation,apoptosis,migration,and invasion of trophoblast ***,the inhibitor of STAT3 pathway,was used to investigate whether the STAT3 pathway mediates the biological function of UCK2 in trophoblast ***:The present study found that UCK2 showed low expression in the placenta of PE patients and PE mouse ***(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)and flow cytometry assays verified that up-regulation of UCK2 promoted the proliferation of trophoblast cells,while the silence of UCK2 suppressed cell ***,flow cytometry and TdT-mediated dUTP Nick-End Labeling assays demonstrated that knockdown of UCK2 resulted in apoptosis of trophoblast *** wound healing and transwell assays showed that the migration and invasion activities of the trophoblast cells were facilitated by the overexpression of UCK2 and were blocked by the silence of ***,the expression of phosphorylated STAT3 was increased with the upregulation of UCK2 and decreased with the inhibition of *** the STAT3 pathway was blocked by its inhibitor stattic,the promotion effects of UCK2 on trophoblast cells were ***:UCK2 promotes the proliferation,migration,and invasion of trophoblast cells,and these effects may be partly mediated by the activation of the STAT3 pathway.

关键词： UCK2 Preeclampsia STAT3 trophoblast Invasion

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

THBS1 regulates trophoblast fusion through a CD36-dependent inhibition of cAMP,and its upregulation participates in preeclampsia

引用

Genes & Diseases 2021年第3期8卷 353-363页

作者： Fu-Mei Duan Li-Juan Fu Yong-Heng Wang Enoch Appiah Adu-Gyamfi Ling-Ling Ruan Zeng-Wei Xu Shi-Quan Xiao Xue-Mei Chen Ying-Xiong Wang Tai-Hang Liu Yu-Bin Ding School of Public Health and Management Chongqing Medical UniversityChongqing400016PR China Department of Bioinformatics The School of Basic MedicineChongqing Medical UniversityChongqing400016PR China The Joint International Research Laboratory of Reproduction and Development Ministry of EducationChongqing400016PR China Department of Herbal Medicine School of Traditional Chinese MedicineChongqing Medical UniversityChongqing400016PR China Department of Reproductive Medicine The Third Affiliated HospitalChongqing Medical UniversityChongqing401120PR China

Preeclampsia is a pregnancy complication which threatens the survival of mothers and *** originates from abnormal placentation,especially insufficient fusion of the cytotrophoblast cells to form the *** this study,we found that THBS1,a matricellular protein that mediates cell-to-cell and cell-to-matrix interactions,is downregulated during the fusion of primary cytotrophoblast and BeWo cells,but upregulated in the placenta of pregnancies complicated by ***,THBS1 was observed to interact with CD36,a membrane signal receptor and activator of the cAMP signaling pathway,to regulate the fusion of cytotrophoblast *** of THBS1 inhibited the cAMP signaling pathway and reduced the BeWo cells fusion ratio,while the effects of THBS1 were abolished by a CD36-blocking *** results suggest that THBS1 signals through a CD36-mediated cAMP pathway to regulate syncytialization of the cytotrophoblast cells,and that its upregulation impairs placental formation to cause ***,THBS1 can serve as a therapeutic target regarding the mitigation of abnormal syncytialization and preeclampsia.

关键词： cAMP Cell fusion Preeclampsia THBS1 trophoblast

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Tissue factor expression and methylation regulation in differentiation of embryonic stem cells into trophoblast

引用

Asian Pacific Journal of Tropical Medicine 2014年第7期7卷 557-561页

作者： Lin-Xin Liu Hui Zeng En-Yi Liu Fang-Ping Chen Department of Hematology Xiangya Hospital of Central South University

Objective:To explore tissue factor(TF)expression and methylation regulation in differentiation of human embryonic stem cells(hESCs)into ***:Differentiation of hESCs into trophoblast was induced by bone morphogenetic protein 4(BMP4).Expression of gene,protein of TF and DNA methylation at different time points during induction process was detected by RTPCT,Western blot,flow cytometry and MSP-PCR ***:The expression of mRNA,protein level of TF could be detected during directional differentiation of hESCs to trophoblast cells,semi methylation-semi non methylation expression appeared at TF DNA promoter region,and it showed decreased methylation level and increased non methylation level with formation of trophoblast cell and increased expression of ***:It shows that during differentiation of hESCs into trophoblast,the differential expression of TF is related with DNA methylation level,and it is changed with the methylation or non methylated *** provids new platform to furtherly explore the regulation mechanisms of specific expression of tissue factor in the process of the embryonic stem cell development.

关键词： Embryonic stem cells trophoblast cell Tissue factor Methylation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effect of hypoxia on expression of placental trophoblast cells SATB1 and β-catenin and its correlation with the pathogenesis of preeclampsia

引用

Asian Pacific Journal of Tropical Medicine 2016年第6期9卷 548-552页

作者： Qiang Wu Gang Wu Jing-Xiang Li Department of Clinical Laboratory Linyi People's Hospital Department of Obstetrics General Hospital of Laiwu Iron and Steel Company Department of Proctology Dongzhimen Hospital Beijing University of Chinese Medicine

Objective: To study the effect of hypoxia on the expression of placental trophoblast cells SATB1 and β-catenin and its correlation with the pathogenesis of preeclampsia. Methods: trophoblastic cell lines HRT8/SVneo were cultured, SATB1 and β-catenin expression and cell biological behavior were determined after hypoxia reoxygenation treatment; cell biological behavior and the expression of related genes were determined after the transfection of SATB1 and β-catenin siR NA; preeclampsia placenta and normal placenta tissues were collected and the expression of SATB1 and β-catenin were determined. Results: OD value, cell migration rate, m RNA contents of SATB1 and β-catenin of H/R group were significantly lower than those of Nor group, cell apoptosis rate was higher than that of Nor group and the number of invasive cells was less than that of Nor group; OD value and bcl-2 mRNA content of SATB1-siRNA group were lower than those of NC group; cell apoptosis rate as well as Bax, Caspase-3, caspase-6 and caspase-9 mRNA contents were higher than those of NC group; cell migration rate as well as CTSB, CTSD, MMP2 and MMP9 mRNA contents of β-catenin-siRNA group were lower than those of NC group; the number of invasive cells was less than that of NC group; the expression levels of SATB1 and β-catenin in preeclampsia placenta tissue were significantly lower than those in normal placenta tissue. Conclusions: Hypoxia can inhibit the expression of SATB1 and β-catenin in the pathogenesis of preeclampsia, which can affect the proliferation, apoptosis, migration and invasion of cells.

关键词： Preeclampsia Placental trophoblast SATB1 Beta-catenin

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Regulation of activin A in cell proliferation and hormone secretion by human normal trophoblast cells

引用

Chinese Science Bulletin 1999年第7期44卷 609-612页

作者： Yanling Wang J. P. Mathe T. Woodroff Shuyi Luo Linzhi Zhuang Chinese Acad Sci Inst Zool State Key Lab Reprod Biol Beijing 100080 Peoples R China Genentech Inc S San Francisco CA 94080 USA

Regulation of activin A in cell proliferation as well as hCG and progesterone secretion was investigated using primary cultured cytotrophoblast cells and normal placenta origin cytotrophoblast cell line-NPC cells in serum-free system. It was shown that activin A promoted hCG and progesterone secretion in primary cultured cytotrophoblast cells as well as progesterone secretion in NPC cells, while it had no effect on cell proliferation and hCG secretion in NPC cells. lmportant evidence is provided for the autocrine regulatory mechanism of activin A on hormone secretion in placental trophoblast cells at early pregnancy.

关键词： human placental trophoblast cells at early pregnancy activin A cell proliferation hCG progesterone.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Propylparaben Induces Reproductive Toxicity in Human Extravillous trophoblast Cells via Apoptosis and Cell Cycle Pathways

引用

Environment & Health 2024年第5期2卷 301-310页

作者： Yifen Guo Yingao Yang Zixuan Zhou Changyuan Zhao Yanchun Li Huanying Zhou Shuyue Ren Yan Gu Zhixian Gao Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety Tianjin Institute of Environmental and Operational MedicineTianjin 300050China Department of Family Planning The Second Hospital of Tianjin Medical UniversityTianjin 300211China

Parabens(PBs),especially propylparaben,commonly used in consumer products,pose environmental and health *** study explored propylparaben’s cytotoxicity on HTR-8/SVneo human trophoblast cells,revealing significant dosedependent cytotoxic effects,particularly post 48-h *** propylparaben levels triggered apoptosis,evidenced by increased Bax and activated Caspase-3,and induced the G0/G1 cell cycle ***,an increase in reactive oxygen species and reduced mitochondrial membrane potential indicated oxidative stress and mitochondrial *** Nacetylcysteine(NAC)treatment reduced oxidative stress,cell invasiveness persisted,suggesting propylparaben might affect cell migration through nonoxidative *** transcriptome analysis through RNA sequencing revealed 3488 differentially expressed genes affected by propylparaben,highlighting changes in pathways like apoptosis and cell cycle regulation and identifying seven hub genes as potential biomarkers for pregnancy-related *** study comprehensively demonstrates the cytotoxic effects of propylparaben on human trophoblast cells,notably through apoptosis induction and cell cycle disruption,thereby providing crucial insights into its potential risks for reproductive health.

关键词： propylparaben HTR-8/SVneo cells trophoblast apoptosis RNA-seq

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effects of maternal diabetes on trophoblast cells

引用

World Journal of Diabetes 2015年第2期6卷 338-344页

作者： Marlúcia Bastos Aires Anne Carolline Veríssimo dos Santos Department of Morphology Federal University of Sergipe

Diabetes mellitus(DM)is a health condition characterized by hyperglycemia over a prolonged *** are three main types of DM:DM type 1(DM1),DM2 and gestational DM(GDM).Maternal diabetes,which includes the occurrence of DM1 and DM2 during pregnancy or GDM,increases the occurrence of gesttional complications and adverse fetal *** hyperglycemic intrauterine environment affects not only the fetus but also the placental development and function in humans and experimental *** underlying mechanisms are still unclear,but some evidence indicates alterations in trophoblast proliferation,apoptosis and cell cycle control in diabetes.A proper coordination of trophoblast proliferation,differentiation and invasion is required for placental ***,increased expression of proliferative markers in junctional and labyrinth zones of rat placentas and villous cytotrophoblast,syncytiotrophoblast,stromal cells and fetal endothelial cells in human placentas is reported among ***,reduced apoptotic index and expression of some apoptotic genes are described in placentas of GDM *** addition,cell cycle regulators including cyclins and cyclin-dependent kinase inhibitors seem to be affected by the hyperglycemic *** studies are necessary to check the balance between proliferation,apoptosis and differentiation in trophoblast cells during maternal diabetes.

关键词： Diabetes Placenta Proliferation Apoptosis Differentiation trophoblast

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Mechanisms Underlying Cell-Fate Specification and Cellular Diversity of the trophoblast Lineage during Placental Morphogenesis in Mice

引用

Reproductive and Developmental Medicine 2021年第4期5卷 220-236页

作者： Jian-Qi Wang Change Mu Yang Sun Jin-Hua Lu Hai-Bin Wang Department of Obstetrics and Gynecology The First Affiliated Hospital of Xiamen UniversitySchool of MedicineXiamen UniversityXiamenFujian 361003China Fujian Provincial Key Laboratory of Reproductive Health Research School of MedicineXiamen UniversityXiamenFujian 361102China

Placental morphogenesis is a highly dynamic process involving mutual recognition and interlacing between the trophoblast-uterus and ultimately the initiation of the maternal-fetal circulatory *** placental morphogenesis in mice,the trophoblast lineage,which integrates maternal and fetal signaling,undergoes stage-specific changes in gene regulatory programs directing cellular proliferation and fate specification,generating diverse trophoblast *** accumulating evidence from studies on genetically engineered and mutant mice has revealed the involvement of cell-specific core transcription factors in certain key events during placental morphogenesis,the precise molecular mechanisms by which multipotent trophoblasts gradually differentiate into different subtypes are still largely *** this review,we primarily focus on mutant mouse models with placental phenotypes to provide a comprehensive understanding of the molecular mechanisms underlying cell-fate specification and cellular diversity of the trophoblast lineage during the placental morphogenesis.

关键词： Differentiation Morphogenesis Multipotency Placenta trophoblast

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Metastin expression in the placenta tissue of preeclampsia and its effect on the trophoblast cell proliferation and apoptosis

引用

Journal of Hainan Medical University 2018年第4期24卷 1-4页

作者： Jia-Yan Wang Department of Obstetrics and Gynecology Ziyang People's Hospital in Shaanxi ProvinceZiyangShaanxi Province725399China

Objective: To investigate the metastin expression in the placenta tissue of preeclampsia and its effect on the trophoblast cell proliferation and apoptosis. Methods: A total of 80 puerperae with preeclampsia who gave birth in this hospital between September 2014 and December 2016 were collected as observation group and 50 normal puerperae who gave birth in the hospital during the same period were collected as normal control group. The metastin protein expression as well as the trophoblast cell proliferation and apoptosis gene expression in placenta tissue were compared between the two groups of puerperae, and Pearson test was used to evaluate the metastin expression in the placenta tissue of preeclampsia and its effect on the trophoblast cell proliferation and apoptosis. Results: Metastin protein expression in the placenta tissue of observation group was significantly higher than that of normal control group. trophoblast cell proliferation genes Notch1 and HLA-G mRNA expression in the placenta tissue of observation group were lower than those of normal control group whereas PHLDA2 mRNA expression was higher than that of normal control group;apoptosis genes bcl-2 mRNA expression was lower than that of control group whereas bax, Fas and caspase-3 mRNA expression were higher than those of normal control group. Pearson test showed that metastin protein expression in the placenta tissue of puerperae with preeclampsia was directly correlated with the trophoblast cell proliferation and apoptosis gene expression. Conclusion: There is abnormally high metastin protein expression in placenta tissue of puerperae with preeclampsia, and it can directly affect the proliferation and apoptosis process of trophoblast cells.

关键词： Preeclampsi Metastin trophoblast cell Proliferation gene Apoptosis gene

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：