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Effects of triterpenic acid from Prunella vulgaris L. on glycemia and pancreas in rat model of streptozotozin diabetes

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Chinese Medical Journal 2013年第9期126卷 1647-1653页

作者： ZHOU Qiao-xia LIU Feng ZHANG Jing-shuo LU Ji-guang GU Zhen-lun GU Gui-xiong Institute of Pediatric Medicine Soochow University Affiliated Children's Hospital Suzhou Jiangsu 215003 China Department of Pediatrics Department of Cardiology Department of Pharmacy Suzhou Kowloon Hospital Affiliated to Shanghai Jiaotong University Suzhou Jiangsu 215021 China Department of Pharmaceutic Analysis College of Pharmaceutical Science Soochow University Suzhou Jiangsu 215123 China Department of Pharmacology Suzhou Institue of Chinese Materia Medica Soochow University Suzhou Jiangsu 215007 China

Background The effects of triterpenic acid from Prunella vulgaris L. （TAP） on diabetes and its mechanism are uncertain. The aim of this study was to investigate the effects of TAP on antihyperglycemic, antioxidant, and pancreas-protective in streptozotozin （STZ）-diabetic rats. Methods The diabetic model was produced by injection of 60 mg/kg STZ. Blood was drawn from the tail vein of rats after 72 hours. Rats with blood glucose 〉16.7 mmol/L were considered diabetic. Diabetic rats were randomly divided into four groups：（1） Diabetes rat （STZ）, （2） Diabetic rats treated with 50 mg/kg of triterpenic acid from Prunella vulgaris L （STZ＋TAP50）, （3） Diabetic rats treated with 100 mg/kg TAP （STZ＋TAP100）, and （4） Diabetic rats treated with 200 mg/kg TAP （STZ＋TAP200）. Normal rats （n=10） acted as the control group （NC）. TAP was administered by the intragastric route once each day for six weeks. Body weight and the concentration of blood glucose （BG） were measured after three and six weeks. Fructosamine （FMN）, malondialdehyde （MDA）, and nitric oxide （NO）, and the activities of nitric oxide synthase （NOS） and superoxide dismutase （SOD） in serum were determined after six weeks using commercially available kits following the manufacturer＇s instructions. Pathologic changes in pancreatic β-cells were also investigated by microscopic examination after hematoxylin-eosin （HE） staining. The level of SOD mRNA in pancreatic β-cells was measured by polymerase chain reaction （PCR）. Results The levels of BG, FMN, NO, and MDA and the activities of NOS in serum in the four diabetes groups were significantly increased compared with the control group （P 〈0.01）. The activity of SOD in serum and the body weight was significantly decreased compared with the control group （P 〈0.01）. After administration of TAP to diabetic rats for six weeks, the body weight and the levels of BG, FMN, MDA, NO and the activity of NOS in serum decreased significantly compared with the STZ group in a dose-dependent manne

关键词： diabetes mellitus oxidative stress lipid peroxidas free radicals antioxidant triterpenic acid Prunella vulgaris L.

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Simultaneous determination of oleanolic acid and ursolic acid by RP-HPLC in the leaves of Eriobotrya japonica Lindl

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Journal of Pharmaceutical Analysis 2012年第3期2卷 238-240页

作者： Xiao-Hong Xu,Qing Sun,Zhi-He Zang School of Pharmacy,Chengdu Medical College,Chengdu 610083,People’s Republic of China School of Pharmacy Chengdu Medical College Chengdu 610083 People's Republic of China.

Oleanolic acid(OA) and ursolic acid(UA) are isomeric triterpenic acids and only one methyl’s position is different between *** and UA always exist in the same plant,so it is difficult to separate them when determining contents by *** this study,a very simple mobile phase for HPLC was developed to simultaneously determine UA and OA,and the factors affecting separation were also *** mobile phase is methanol:water(95:5) with flow rate 0.4 mL/*** retention time for OA and UA was 20.58 and 21.57 min,respectively,the resolution was *** average contents of OA and UA of three Loquat leaves sets were 1.4 mg/g and 5.6 mg/g,*** the HPLC,we found that changing mobile phase,adjusting the pH value or adding ion-pairing agent could not affect the separation between UA and OA *** adjustment of the flow rate and column temperature could improve the resolution greatly.

关键词： RP-HPLC Oleanolic acid(OA) Ursolic acid(UA) triterpenic acid

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