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Spectrophotometric Determination of trimethylamine-nitrogen in Cadaver Tissues for the Estimation of Late Postmortem Interval:A Pilot Study

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2008年第6期28卷 630-633页

作者：刘茜蔡向阳刘艳周兰易少华刘良 Faculty of Forensic Medicine Tongji Medical CollegeHuazhong University of Science and Technology Key Laboratory of Evidence Science (China University of Political Science and Law) Ministry of Education

To study the relationship between the late postmortem interval （PMI） and trimethyl-amine-nitrogen （TMA-N） in postmortem tissues of cadaver, TMA-N in muscles, livers and kidneys of rats was measured at different postmortem intervals （PMI） by using a modified spectrophotometric method. The results indicated that the detection sensitivity of TMA-N was 1 mg/L, and there was a good linear correlation between the value of absorbance （A value） and TMA-N at the concentration of 1-10 mg/L （R2=0.9991）. Although TMA variation in muscles was different from that in inner organs during the time since death, TMA-N changes in cadaver tissues was positively correlated with PMI. During 2 to 7d since death, the best correlation between PMI and TMA-N concentration was found in muscles. With PMI as an independent variable, the cubic polynomial regression equation was y=-0.457x3 ＋6.519x2-24.574x＋27.207 （R2=0.969）. During 3 to 8 days since death, PMI was best correlated with TMA-N concentration in inner organs. With PMI as the independent variable, the cubic polynomial regression equation was y=0.509x3-9.153x2＋55.727x-95.819 （R2=0.953）. It was concluded that TMA-N in tissues could be used as a new estimator for late PMI. The method used in this study offered advantages such as accuracy, sensitivity, little samples required and wide PMI estimation.

关键词： forensic medicine postmortem interval spectrophotometry trimethylamine-nitrogen spoilage product

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