检索结果-南通市图书馆

trigonelline protects the cardiocyte from hydrogen peroxide induced apoptosis in H9c2 cells

Asian Pacific Journal of Tropical Medicine 2015年第4期8卷 263-268页

作者： Soundharrajan Ilavenil Da Hye Kim Young-Il Jeong Mariadhas Valan Arasu Mayakrishnan Vijayakumar Ponnuraj Nagendra Prabhu Srisesharam Srigopalram Ki Choon Choi Grassland and Forage division National Institute of Animal ScienceRDA The United Graduate School of Agricultural Sciences Tottori University School of Medicine Pusan National University Department of Botany and Microbiology Addiriyah Chair for Environmental StudiesCollege of ScienceKing Saud University Department of Animal Sciences University of Illinois Department of Horticulture Sunchon National University

Objective:To elucidate the key parameters associated with hydrogen peroxide induced oxidative stress and investigates the mechanism of trigonelline(TG)for reducing the H_2O_2induced toxicity in H9c2 ***:Cytotoxicity and antioxidant activity of TG was assessed by EZ-CYTOX *** extraction and cDNA synthesized according to the kit manufacture *** was measured by the Flowcytometry,general PCR and ***:It was found that the TG significantly rescued the morphology of the H9c2 *** of cells with TG attenuated H_2O_2 induced cell deaths and improved the antioxidant *** addition,TG regulated the apoptotic gene caspase-3,caspase-9 and anti-apoptotic gene ***-XL during H_2O_2 induced oxidative stress in H9c2 *** results were comparable with quercetin *** evident,flow cytometer results also confirmed the TG significantly reduced the H_2O_2 induced necrosis and apoptosis in H9c2 ***,further increment of TG concentration against H_2O_2 could induce the necrosis and apoptosis along with H_2O_***:It is suggested that less than 125μM of TG could protect the cells from H_2O_2 induced cell damage by down regulating the caspases and up regulating the Bcl-2 and Bcl-XL ***,we suggest the trigonelline could be useful for treatment of oxidative stress mediated cardiovascular diseases in future.

关键词： trigonelline H9c2 cells Antioxidant Apoptosis Cardiovascular diseases

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Protection effect of trigonelline on liver of rats with non-alcoholic fatty liver diseases

引用

Asian Pacific Journal of Tropical Medicine 2015年第8期8卷 639-642页

作者： Dong-Fang Zhang Fan Zhang Jin Zhang Rui-Ming Zhang Ran Li Department of Pharmacy Shandong Liaocheng People’s Hospital Department of Hepatobiliary Surgery Affiliated Hospital of Binzhou Medical University Department of Neurology Shandong Liaocheng People’s Hospital Liaocheng University

Objective: To study the effect of trigonelline on the change of indicators of serum transaminase, lipoprotein and liver lipid of model rats with non-alcoholic fatty liver diseases and on the expression level of Bcl-2 and Bax proteins. Methods: A total of 45 SD rats were randomly divided into Fthe control group, model group and trigonelline intervention group. Rats in the control group were fed with the common diet, while rats in the model group and intervention group were fed with the high fat diet. 8 weeks later, the intervention group received the intragastric administration of trigonellin e(with the dosage of 40 mg/kg/d) for 8 weeks; while control group and model group received the intragastric administration of saline with the equal dosage. Blood was taken from the abdominal aorta of rats 8 weeks later, detecting the level of a series of indicators of ALT, AST, TG, TC, HDL-C and LDL-C in the serum. After the rats were sacrificed, detect the indicators of TG, TC, SOD and MDA in the liver tissue of rats, as well as the expression of Bcl-2 and Bax in the liver tissue. Results: Results of histopathologic examination showed that the damage degree of liver for rats in the trigonellineintervention group was smaller than the one in the model group, with significantly reduced hepatic steatosis and the partially visible hepatic lobule. The levels of ALT, AST, TC and LDL-C in the serum of rats in the trigonelline group were significantly reduced, while the change in the levels of TG and HDL-C was not significantly different. The levels of TG, TC and MDA in the liver tissues were significantly decreased, while the level of SOD significantly increased; the expression of Bcl-2 protein in the liver tissues of rats in the trigonelline intervention group was significantly increased, while the expression of Bax protein significantly decreased. Conclusions: The trigonelline contributes to the therapeutic effect of non-alcoholic fatty liver diseases. It can also increase the expres

关键词： trigonelline Non-alcoholic fatty liver diseases Bcl-2 Bax

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Stress Responses of Peanut (<i>Arachis hypogaea</i>L.) Genotypes as Measured by trigonelline Content after Exposure to UV-B Radiation

引用

American Journal of Plant Sciences 2017年第5期8卷 998-1010页

作者： David Willmon Amith R. Devireddy Madhuri Inupakutika Naveen Puppala Youngkoo Cho Department of Biology Eastern New Mexico University Portales NM USA Department of Biological Sciences University of North Texas Denton TX USA Agricultural Science Center New Mexico State University Clovis NM USA

UV-B radiation has been widely documented as a stressor for plants that can cause decreased biomass, reduction in photosynthesis, and oxidative stress. trigonelline is a secondary metabolite that is biosynthesized in some plants in response to abiotic stress such as UV-B irradiation. The objectives of this study were to examine biochemical stress responses for peanut plants (Arachis hypogaea L.) of four different genotypes (Spanish, Valencia, Virginia, and Runner) after exposure at various lengths to UV-B radiation and to examine the alteration of trigonelline biosynthesis due to the age of the plants. Peanut plants from the genotypes were exposed to UV-B radiation at three exposure times (60, 120, and 180 min);plants from two growth stages, the flowering (R1) and early maturity (R7), were used. Significant positive correlations (rs 0.29-0.74, P≤0.05) were found for trigonelline concentrations and UV-B exposure times. With longer exposure times of 180 min for plants at R7, trigonelline biosynthesis began as early as 10 days after treatment with 154.6 μg·g-1 DW and remained or increased by up to 71.5 μg·g-1 DW (46.3%) throughout the sampling intervals (10, 20, 30, 40, and 50 days after treatment) to a final value of 226.1 μg·g-1 DW. All four genotypes at R7 exhibited trigonelline concentrations 47.3% to 52.4% (71.6 to 96.5 μg·g-1 DW) higher than individuals at R1. trigonelline biosynthesis at R7 was significantly (Ptrigonelline concentrations at R1 were significantly influenced (Ptrigonelline concentration among the four different genotypes. UV-B irradiation had the greatest effect on plants at R7 after 120 and 180 min of exposure, as 15 out of 20 (75%) individuals had significantly higher (Ptrigonelline concentrations.

关键词： Abiotic Stress Arachis hypogaea L. trigonelline UV-B

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Increase of trigonelline in Trigonella persica Plant under Drought Stress

引用

Journal of Botanical Research 2022年第2期4卷 19-25页

作者： Fatemeh Zarinkamar Reyhaneh Medhat Maryam Rezayian Department of Plant Biology Faculty of Biological BiologyTarbiat Modares UniversityTehranIran

Trigonella persica is a valuable medicinal plant which comprises trigonelline that is secondary metabolite and important component in cosmetic and *** research was conducted in order to evaluation the drought stress effect on growth parameters,root anatomical changes and trigonelline content in *** were grown under soil moisture corresponding to 100%,75%,50%and 25%field capacity for two *** data showed that drought stress was significantly decreased fresh weight and dry weight of shoot and *** addition,leaf area was declined due to drought ***,root length was enhanced by drought *** microscopic study demonstrated that drought stress increased thickness of epidermal,endodermal,vascular bundle,central cylinder and parenchyma in *** stress caused a significant increment in alkaloid and trigonelline content in aerial parts and roots of *** results revealed that *** responded to drought stress by increasing the alkaloid and trigonelline,as well as the anatomical changes in *** the importance of trigonline and alkaloids,this work may open prospects for production of the pharmaceutically valuable secondary metabolites thereby drought stress.

关键词： Anatomical changes Drought Root Trigonella persica trigonelline

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Quantitative analysis of trigonelline in some Annona species by proton NMR spectroscopy

引用

Natural Products and Bioprospecting 2013年第4期3卷 158-160页

作者： Alan Rodrigues Teixeira MACHADO Gisele Avelar LAGE Felipe da Silva MEDEIROS José Dias de Souza FILHO Lúcia Pinheiro Santos PIMENTA Departamento de Química ICExUniversidade Federal de Minas GeraisBelo HorizonteMinas Gerais31270-901Brasil

A quantitative ^(1)H NMR method(qHNMR)was used to measure the trigonelline content in the leaves of six species of the Annona *** methodology employed compared the intensities of the signals at δ9.14(H-2)andδ0.00,the internal standard TSP-d_(4).This measuring method was able to establish the concentration of trigonelline in the range from 0.67 to 10.04 mg·g^(-1) depending on the investigated extract.

关键词： trigonelline Annona quantitative^(1)H NMR

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Antibacterial Effect of Aqueous Extracts and Bioactive Chemical Compounds of <i>Coffea canephora</i>against Microorganisms Involved in Dental Caries and Periodontal Disease

引用

Advances in Microbiology 2014年第14期4卷 978-985页

作者： Flávia M. da Silva Natalia L. P. Iorio Leandro A. Lobo Kátia Regina N. dos Santos Adriana Farah Lucianne C. Maia Andréa G. Antonio Departamento de Pós-Graduacao Mestrado Profissional em Odontologia Faculdade de Odontologia Universidade Veiga de Almeida Rio de Janeiro Brasil Departamento de Ciência Básica Universidade Federal Fluminense Nova Friburgo Brasil Departamento de Microbiologia Médica Instituto de Microbiologia Universidade Federal do Rio de Janeiro Rio de Janeiro Brasil Instituto de Nutricao Universidade Federal do Rio de Janeiro Rio de Janeiro Brasil Departamento de Odontopediatria e Ortodontia Faculdade de Odontologia Universidade Federal do Rio de Janeiro Rio de Janeiro Brasil

This study aimed to evaluate the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of Coffea canephora aqueous extract (Cc), trigonelline (Tg) and 5-caffeoylquinic acid (5-CQA) against cariogenic microorganisms: Streptococcus parasanguinis ATCC 903 (SP), Lactobacillus rhamnosus ATCC 9595 (LR);and pathogens related to periodontal disease: Porphyromonas gingivalis ATCC 33277 (PG), Fusobacterium nucleatum ATCC 25586 (FN), Prevotella intermedia ATCC 49046 (PI) and Prevotella nigrescens ATCC 33563 (PN). Different concentrations of Cc (0.15625 to 10 mg/mL), Tg and 5-CQA (0.005 to 10.24 mg/mL) were tested. Chlorhexidine (0.05%) was used as positive control and the substances without the inoculum comprised the blank control. The Cc showed bacteriostatic action against SP (MIC = 5 mg/mL) and LR (MIC = 10 mg/mL). Tg showed bacteriostatic action against SP (MIC = 2.56 to 1.28 mg/mL), LR (MIC = 2.56 mg/mL), PG (MIC = 2.56 to 1.28 mg/mL), FN (MIC = 5.12 mg/mL), PN (MIC = 2.56 mg/mL), and PI (MIC = 2.56 to 1.28 mg/mL). Also, Tg showed bactericide properties against SP (MBC = 2.56 mg/mL), PG (MBC = 2.56 mg/mL), FN (MBC = 10.24 mg/mL), PN (MBC = 5.12 mg/mL), and PI (MBC = 2.56 mg/mL). Although 5-CQA has previously shown activity against Streptococcus mutans, in the present study, it showed no activity against all tested microorganisms. C. canephora extract only showed antibacterial activity against cariogenic microorganisms, not presenting action against periodontal pathogens. It was concluded that trigonelline presented the best effect against all pathogens tested, therefore coffee extracts with higher trigonelline content should be tested against these specific pathogens.

关键词： Oral Pathogens Dental Caries Periodontal Disease Coffee trigonelline Chorogenic Acid

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：