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Real-time-guided bone regeneration around standardized critical size calvarial defects using bone marrow-derived mesenchymal stem cells and collagen membrane with and without using tricalcium phosphate: an in vivo microcomputed tomographic and histologic e

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International Journal of Oral Science 2016年第1期8卷 7-15页

作者： Khalid Al-Hezaimi Sundar Ramalingam Mansour Al-Askar Aws S ArRejaie Nasser Nooh Fawad Jawad Abdullah Aldahmash Muhammad Atteya Cun-Yu Wang Engineer A.B.Growth Factors and Bone Regeneration Research Chair and 3D Imaging Center College of DentistryKing Saud UniversityRiyadhSaudi Arabia Department of Periodontics and Community Dentistry College of DentistryKing Saud UniversityRiyadhSaudi Arabia Department of Oral and Maxillofacial Surgery College of DentistryKing Saud UniversityRiyadhSaudi Arabia Department of Substitutive Dental Sciences College of DentistryUniversity of DammamDammamSaudi Arabia Stem Cell Unit Department of AnatomyCollege of MedicineKing Saud UniversityRiyadh Division of Oral Biology and Medicine School of DentistryUniversity of California at Los AngelesLos AngelesUSA

The aim of the present real time in vivo micro-computed tomography （pCT） and histologic experiment was to assess the efficacy of guided bone regeneration （GBR） around standardized calvarial critical size defects （CSD） using bone marrow-derived mesenchymal stem cells （BMSCs）, and collagen membrane （CM） with and without tricalcium phosphate （TCP） graft material. In the calvaria of nine female Sprague-Dawley rats, full-thickness CSD （diameter 4.6 mm） were created under general anesthesia. Treatment-wise, rats were divided into three groups. In group 1, CSD was covered with a resorbable CM; in group 2, BMSCs were filled in CSD and covered with CM; and in group 3, TCP soaked in BMSCs was placed in CSD and covered with CM. All defects were closed using resorbable sutures. Bone volume and bone mineral density of newly formed bone （NFB） and remaining TCP particles and rate of new bone formation was determined at baseline, 2, 4, 6, and 10 weeks using in vivo pCT. At the lOth week, the rats were killed and calvarial segments were assessed histologically. The results showed that the hardness of NFB was similar to that of the native bone in groups I and 2 as compared to the NFB in group 3. Likewise, values for the modulus of elasticity were also significantly higher in group 3 compared to groups 1 and 2. This suggests that TCP when used in combination with BMSCs and without CM was unable to form bone of significant strength that could possibly provide mechanical ＂lock＂ between the natural bone and NFB. The use of BMSCs as adjuncts to conventional GBR initiated new bone formation as early as 2 weeks of treatment compared to when GBR is attempted without adiunct BMSC therapy.

关键词： bone marrow-derived mesenchymal stem collagen membrane critical size defect guided bone regeneration tricalciumphosphate

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