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Fumonisin-Producing Fusarium from Maize Grains in tretep, Indonesia

Journal of Food Science and Engineering 2013年第10期3卷 534-540页

作者： Petrus Adityo Nugroho Francis Maria Constance Sigit Setyabudi Baharudin Saleh Endang SutriswatiRahayu Faculty of Agricultural Technology Universitas Gadjah Mada Bulaksumur Yogyakarta Indonesia School of Biological Science Universiti Sains Malaysia Penang Malaysia

Fusarium species commonly occur in maize are fungal pathogen which produce mycotoxins, such as fumonisin, trichothecene and zearalenone. In this study, Fusarium species were isolated from maize kernel from tretep, maize producer region and were identified based on microscopic- and macroscopic characters as well as molecular characters using PCR assays and the partial sequence of TEF 1-α gene （Translation Elongation Factor 1-α. The fumonisin-producing ability of these Fusarium was determined by growing them in corn medium and analyzed their fumonisin by ELISA （enzyme-linked immuno assay）. Among 9 isolates, three of them were identified as Fusarium verticillioides, two as Fusarium temperatum, two as Fusarium globosum, one as Fusarium proliJeratum and one as Fusarium subglutinans. Fusarium temperatum is similar morphologically to F. subglutinans, however, both of their differences can be found by molecular analysis. Fumonisin-producing abilities of Fusarium were determined in concentrations 20.51 pg/g-1,109.74 pg/g medium with the highest producer was identified as F. globosum.

关键词： Fusarium tretep identification molecular TEF-1α fumonisin.

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