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Isolation, characterization and modulatory potentials of β-stigmasterol, ergosterol and xylopic acid from Anchomanes difformis on mitochondrial permeability transition pore in vitro

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Chinese Herbal Medicines 2023年第4期15卷 533-541页

作者： Kehinde Oluseun Sodeinde Akinwunmi Oluwaseun Adeoye Adedeji Adesipo Adebayo AAdeniyi John Adeolu Falode Tajudeen Olabisi Obafemi Samuel Olalekan Olusanya Linette Twigge Jeanet Conradie Timothy O.Mosaku Department of Chemistry Federal University Oye-EkitiEkiti State 371104Nigeria Biomembrane Toxicology and Drug Discovery UnitDepartment of BiochemistryFederal University Oye-EkitiEkiti State 371104Nigeria Department of Biochemistry Afe Babalola UniversityAdo-EkitiEkiti State 360001Nigeria Department of Chemistry University of Free StateBloemfontein 9301South Africa Department of Civil Engineering Covenant UniversityOgun State 112104Nigeria

Objective: Secondary metabolites and polyphenolic compounds from medicinal plants have been demonstrated to have multiple biological functions with promising research and development prospects. This study examined the effect of β-stigmasterol(with ergosterol) and xylopic acid isolated from Anchomanes difformis on liver mitochondrial permeability transition pore(mPTP).Methods: The compounds were isolated by vacuum liquid chromatography. Mitochondrial swelling was assessed as changes in absorbance under succinate-energized ***:1H and13C NMR spectroscopic elucidation of the isolates affirmed the presence of β-stigmasterol with ergosterol(1:0.3) and xylopic acid. The isolates reversed the increase in lipid peroxidation and inhibited the opening of mitochondrial permeability transition pores caused by calcium and *** inhibition of m PTP offers a promising therapeutic target for the treatment of mitochondrial-associated ***: Reduction in the activity of calcium ATPase and the expression of Caspase-3 and-9 were observed, suggesting that they could play a role in protecting physicochemical properties of membrane bilayers from free radical-induced severe cellular damage and be useful in the management of diseases where much apoptosis occurs.

关键词： Anchomanes difformis(Blume)Engl ergosterol mitochondrial membrane modulation β-stigmasterol transition pore xylopic acid

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Jatonik polyherbal mixture induced rat liver MMPT pore opening in normal Wistar rat:In vitro and in vivo studies

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Chinese Herbal Medicines 2024年第1期16卷 113-120页

作者： Olabinri P.Folashade Ibrahim Damilare Boyenle Tolulope A.Oyedeji Fiyinfoluwa Demilade Ojeniyi Adisa Ayobami Damilare Leonard O.Ehigie Adeola Folasade Ehigie Membrane Biochemistry and Biophysics Research Laboratory Department of BiochemistryLadoke Akintola University of TechnologyOgbomoso 210214Nigeria Computational Biology/Drug Discovery Laboratory Department of BiochemistryLadoke Akintola University of TechnologyOgbomoso 210214Nigeria College of Health Sciences Crescent University AbeokutaAbeokuta 111105Nigeria Department of Biochemistry University of LagosLagos 101017Nigeria

Objective:To assess acute toxicity,the in vitro and in vivo effects of methanol and ethyl acetate extracts(JME and JEE)of Jatonik polyherbal mixture on some mitochondria-related parameters and their effect on the activity of some liver ***:Acute toxicity of JME and JEE was determined using Lorke’s *** vitro and in vivo opening of the mitochondrial membrane permeability transition pore(MMPT pore)was spectrophotometrically *** of malondialdehyde(MDA)as an index of lipid peroxidation and the activity of mitochondrial ATPase was evaluated in vitro and in vivo and the effect of JME and JEE on the activity of liver enzymes such as alkaline phosphatase(ALP),aspartate and alanine aminotransferase(AST and ALT)and gamma-glutamyl transferase(GGT)was also ***:JME had an LD_(50) of 3808 mg/kg b.w whereas JEE had an LD_(50) greater than 5000 mg/kg *** *** the rats have been fed with both extracts,a photomicrograph of a piece of liver tissue showed no apparent symptoms of *** the in vitro and in vivo studies,both extracts prompted intact mitochondria to open their MMPT *** compared to the control,lipid peroxide product release and ATPase activity were significantly increased(Ppore induction,and enhanced ATPase activity,but an increased MDA ***:Jatonik extracts may be a promising target for drug development in diseases where there is dysregulation of apoptosis,however,further studies are needed to better clarify the molecular mechanism involved in these phenomena.

关键词： acute toxicity Ageratum conyzoides Linn. apoptosis Hunteria umbellate(K.Schum.)Hallier f. Lepidium meyenii Walp. mitochondrial ATPase mitochondrial lipid peroxidation mitochondrial membrane permeability transition pore polyherbal mixture Xylopia aethiopica(Dunal)A.Rich

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