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Sexual compatibility of transgenic soybean and different wild soybean populations

Journal of Integrative Agriculture 2022年第1期21卷 36-48页

作者： HU Yu-qi SHENG Ze-wen LIU Jin-yue LIU Qi QIANG Sheng SONG Xiao-ling LIU Biao Weed Research Laboratory College of Life SciencesNanjing Agricultural UniversityNanjing 210095P.R.China Nanjing Institute of Environmental Sciences Ministry of Ecology and EnvironmentNanjing 210042P.R.China

The introduction of genetically modified(GM) soybean into farming systems raises great concern that transgenes from GM soybean may flow to endemic wild soybean via pollen. This may increase the weediness of transgenic soybean by increasing the fitness of hybrids under certain conditions and threaten the genetic diversity of wild soybean populations. Although pollen-mediated gene flow between GM crops and wild relatives is dependent on many factors, the sexual compatibility(SC)determined by their genetic backgrounds is the conclusive factor. The considerable genetic variation among wild soybean populations may cause compatibility differences between different wild and cultivated soybeans. Thus, an evaluation of the SC between transgenic soybean and different wild soybeans is essential for assessing the environmental consequences of cultivated soybean–wild soybean transgene flow. The podding and seed sets were assessed after artificial hybridization using transgenic glyphosate-resistant soybean as the paternal parent and 18 wild soybean populations as the maternal parents. Then, the average number of filled seeds produced in 200 flowers(AFS) was calculated for each wild soybean under natural self-pollination as well as under artificial crossing with transgenic soybean. Finally, the index of cross-SC was calculated(ICSC) as the ratio of the AFS of wild soybean artificially crossed with transgenic soybean and the AFS of naturally self-pollinated wild soybean. The results demonstrated that after self-pollination and crossing with transgenic soybean, the average podding rates of 18 wild soybean populations ranged within 96.50–99.50% and 4.92–18.03%, and the average filled seed numbers per pod varied from 1.70 to 2.69 and 0.20 to 0.48, respectively. The results showed that approximately 89% of wild soybeans displayed either medium or higher than medium SC with transgenic soybean(ICSC>1.0%). This implied the high possibility of gene flow via pollen from transgenic soybean t

关键词： transgenic soybean wild soybean sexual compatibility artificial hybridization gene flow

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Agronomic Traits of transgenic soybean Overexpressing TaDREB3

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Journal of Northeast Agricultural University(English Edition) 2011年第2期18卷 1-6页

作者： ZHANG Binbin ZHANG Tong WU Xiaoxia Key Laboratory of soybean Biology in Chinese Ministry of Education Northeast Agricultural University Harbin 150030 China

Dongnong50 was overexpressing TaDREB3, a stress-tolerance gene, and transgenic progenies with TaDREB3 showing stable heredity characters were obtained in this study. The seeds of T4 generation were sowed on saline land of which salt concentration was 0.19% and pH was 8.43, and agronomic traits including growth period, plant height, numbers of main nodes, branching numbers, pods per plant, numbers of seeds per plant, and weight of 100-seed were collected and comparing to control plants Dongnong50 were made. The impacts of TaDREB3 gene on agronomic traits of soybeans under high concentration of salt were discussed. The results showed that the growth period was not altered in transgenic line with TaDREB3 under high concentration of saline and alkali, the extremely significant or significant increase in the numbers of main nodes, pods per plant and in branching numbers and other agronomic traits in transgenic lines in comparing with non-transgenic lines was an indicator of their resistance to saline-alkali stress

关键词： DREB transgenic soybean stress-tolerance growth and development period agronomic trait

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Regeneration Function Analysis of GmESR1 in transgenic soybean

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Journal of Northeast Agricultural University(English Edition) 2020年第2期27卷 1-8页

作者： Li Mo Pan Xiao-cheng Liu Yang Li Yuan-ming Li Hong-wei Liu Wei Su An-yu Wu Xiao-xia College of Agriculture Northeast Agricultural UniversityHarbin 150030China 65301 Farm and Sideline Bases of Army Wudalianchi 164100HeilongjiangChina College of Resources and Environmental Sciences Northeast Agricultural UniversityHarbin 150030China College of Heilongjiang Green Food Research Institute Harbin 150030China

ENHANCER OF SHOOT REGENERATION(ESR1)is an important regulator of plant regeneration in vitro,which promotes regeneration of *** this study,transgenic positive plants with normal expression of proteins were screened by molecular *** the study of the transgenic plants and the control Dongnong 50,the difference between immature embryo-induced callus and induced shoot bud was *** increase in callus weight indicated that GmESR1 gene accelerated the formations of shoot *** measuring the changes of hormone in the process of induction callus of transgenic plants,it was found that the contents of indole-3-acetic acid(IAA)and zeatin(ZT)in transgenic lines were significantly *** could be concluded that GmESR1 gene promoted the accumulation of hormone and affected regeneration *** addition,this study also verified the interaction between GmBIM1 gene and GmESR1 gene by bimolecular fluorescence complementation(BiFC).

关键词： transgenic soybean GmESR1 regeneration indole-3-acetic acid(IAA) zeatin(ZT)

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Establishment of TaqMan Real-time Quantitative PCR Assay for Foreign Gene Copy Numbers in transgenic soybean

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Journal of Northeast Agricultural University(English Edition) 2012年第4期19卷 48-52页

作者： Qiu You-wen Gao Xue-jun Qi Bang-ruo Li Lu Zhen Zhen Life Science and Biotechnique Research Center Northeast Agricultural University/Testing and Inspecting Center for GMO

TaqMan quantitative PCR technique was used to detect the copies of exogenous CaMV35S flanks sequence in transgenic soybean. With soybean lectin as the endogenous reference gene, and gene complex DNA in non-GMO soybeans as the endogenous reference standard, the gradient dilution method was used to separately calculate Ct value of endogenous reference gene and plasmid DNA and correlation standard curve equation of logarithm of copies, and then to calculate the copies of samples through substituting thus-obtained Ct into the standard curve equation. The standard curve equation of endogenous reference gene was y =–3.422x＋35.201, R2=0.998; the standard curve equation of exogenous gene was y =–3.495x＋35.303, R2=0.999. The sample copies was got by putting Ct value into the standard curve equation, and it was the ratio of exogenous gene and reference gene. We found that CaMV35S gene in transgenic soy was single copy.

关键词： real-time PCR transgenic soybean copy lectin CaMV35S flanking sequence

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Panax ginseng PgTIP1 gene confers enhanced salt and drought tolerance to transgenic soybean plants through maintaining homeotasis of water,salt ions and ROS

Panax ginseng PgTIP1 gene confers enhanced salt and drought ...

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第七届长三角植物科学研讨会暨青年学术报告会

作者： J.An C.Cheng Z.Hu H.Chen W.Cai B.Yu Lab of Plant Stress Biology College of Life SciencesNanjing Agricultural University Institute of Plant Physiology and Ecology Shanghai Institutes for Biological SciencesUniversity of Chinese Academy of SciencesChinese Academy of Sciences

Panax ginseng PgTIP1 gene was formerly demonstrated to possess high water channel activity by its heterologous expression in Xenopus laevis oocytes and in yeast;and also plays significant roles in enhanced salt/drought tolerance of Pg TIP1-transgenic Arabidopsis plants and salt adaptation of Pg TIP1-transformed Glycine *** this work,Pg TIP1 gene was further transformed into the hybrid strain 4076(F),which was selected for salt tolerance generation by generation from the cross assembly *** N23674×*** BB52 *** Pg TIP1-transgenic soybean plants mediated by the pollen tube pathway,represented by the lines L19 and L29,were simultaneously analyzed at the physiological and molecular levels for enhanced salt and drought *** results showed that,the salt-stressed Pg TIP1-transgenic L19 and L29 have acquired better leaf stomatal movement and water-gas exchange capacity,obvious drops of absorption,transport and accumulation of Na,Cland Na/K ratio,and enhanced antioxidase activities of SOD,POD,CAT,and APX in the roots or leaves in contrast to *** suffered from PEG-induced drought stress,the strengthened leaf water-retention capacity and the alleviated cell membrane damages of roots and leaves were displayed for Pg TIP1-transgenic soybean ***,the Pg TIP1-transgenic soybean lines could exhibit salt and drought tolerance together,which are not only related to the primary functions of Pg TIP1 gene involved in water status regulation,but also to its secondary roles in up-regulated expression of stress-related genes(Gm NHX1,Gm SOS1,Gm CLC1,Gm CAT1,Gm APX1,Gm NCED1 and Gm P5 CS1),which are involved in homeostasis of Na,K,and Cl,ROS scavenging,and synthesis of ABA and proline,etc.

关键词： PgTIP1 transformation salt tolerance drought tolerance transgenic soybean water relations ion homeostasis ROS scavenging

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Resistance identification of bivalent fungi-resistant genes transformed soybean to Phytophthora sojae

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Journal of Northeast Agricultural University(English Edition) 2007年第3期14卷 212-217页

作者： GUO Yushuang ZHANG Yanju ZHU Yanming LIU Jia LI Jie BAI Xi College of Life Sciences Northeast Agricultural University Harbin 150030 China College of Agriculture Northeast Agricultural University Harbin 150030 China

soybean is one of the most important sources of edible oil and proteins in the world. However, it suffers from many kinds of fungal diseases which is a major limiting factor in soybean production. The fungal disease can be effectively controlled by breeding plant cultivars with genetic transformation. In this study, the resistance to Phytophthora sojae of five bivalent transgenic soybean lines was identified using the hypocotyls inoculation technique. The lines were the T2 of the transgenic soybean which were transformed with kidney bean chitinase gene and barley ribosome inactivating protein gene, and were positive by Southern Blot analysis. The resistance difference was studied through comparing the death percentage of transgenic soybean with the control. The results showed that four lines were more resistant to P sojae, whereas other one had no significant difference in comparison with the control. These transgenic soybean lines with enhanced resistance to P sojae will be useful in soybean resistance breeding.

关键词： transgenic soybean kidney bean chitinase gene barley ribosome inactivating protein gene soybean Phytophora root rot resistance identification

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Effects of transgenic DREB Toybean Dongnong50 on Diversity of Soil Nitrogen-fixing Bacteria

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Journal of Northeast Agricultural University(English Edition) 2015年第1期22卷 1-11页

作者： Zhang Chun-miao Dong Lei Jin Yu Qu Juan-juan College of Resources and Environment Northeast Agricultural University

Drought is a bottleneck for worldwide soybean production which is getting more serious as the climate continues to worsen. Dehydration responsive element binding（DREB） is a kind of transcription factor that regulates the expression of stress tolerance-related genes in response to drought, high salinity and cold stress in plant. soybean with DREB gene possesses the drought resisting capability which is helpful to increase the yield. However, the potential risk of genetically modified plants（GMPs） on soil microbial community is still in debate. In order to understand the effects of transgenic DREB soybean on the nitrogen-fixing bacteria, the diversity of nif H gene in pot experiments planted transgenic soybean and near-isogenic nontransgenic soybean under normal water condition and drought stress condition was analyzed by PCR-DGGE and sequence analysis. The results showed that transgenic soybean under normal water condition decrease the diversity of the nitrogen-fixing bacteria in the seeding stage and flowering stage, but had no notable effect in other stages. Under drought stress, transgenic soybean reduced the diversity of the nitrogen-fixing bacteria in the flowering stage, but had no notable effects on other stages. Phylogenic analysis revealed that g7, g13, g15 and g19 had a close relationship with Alphaproteobacteria, g12 had a close relationship with Azonexus, others were related to Betaproteobacteria and Burkholderia.

关键词： transgenic soybean DREB nitrogen-fixing bacteria nifH PCR-DGGE

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