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第十届全国免疫学学术大会

作者： Ping Zhu Guangdong Cardiovascular Institute Guangdong General Hospital Guangdong Academy of Medical Sciences

Objective:The development of an animal model bearing definite antigens is important to facilitate the evaluation and modulation of specific allo-antigen responses after ***:In the present study,heterotopic cardiac transplantation was performed from F344/EGFPTg and F344/HLAB27Tg rats to F344 ***:The F344 recipients accepted the F344/EGFPTg transplants,whereas they rejected the cardiac tissue from the F344/HLA-B27Tg rats by 39.4±6.5 days,due to high production of anti-HLA-B27 Ig M-and Ig G-specific *** addition,immunization of F344 rats with skin grafts from F344/HLA-B27Tg rats resulted in robust production of antiHLA-B27 Ig M and Ig G antibodies and accelerated the rejection of a secondary cardiac allograft(7.4±1.9 days).Of interest,the F344 recipients rejected cardiac grafts from double transgenic F344/HLA-B27&EGFPTg rats within 9.0±3.2days,and this was associated with a significant increase in the infiltration of lymphocytes by day 7,suggesting a role for cellular immune *** acid(EPA),one of the w-3 polyunsaturated fatty acids in fish oil,could attenuate the production of anti-HLA Ig G antibodies and B-cell proliferation,significantly prolonging double transgenic F344HLA-B27&EGFPTg to F344 rat cardiac allograft survival(36.1±13.6 days).Moreover,the m RNA expression in the grafts was assessed by q RT-PCR,revealing an increase in the expression of the HO-1,IL-10,TGF-b,IDO,and Foxp3genes in the EPA-treated ***:Our data indicate that HLA-B27 and/or GFP transgenic proteins could establish a unique animal transplantation model to clarify the mechanism of allogeneic cellular and humoral immune response,in which the transplant antigens are specifically *** also demonstrated that EPA was effective in the treatment of rat cardiac allograft rejection and may develop a novel immunomodulatory strategies for organ transplantation.

关键词： Allograft GFP HLA-B27 Regulatory T cell Tolerance transgenic rat

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Eicosapentenoic acid attenuates allograft rejection in an HLA-B27/EGFP transgenic rat cardiac transplantation model

Eicosapentenoic acid attenuates allograft rejection in an HL...

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第九届全国免疫学学术大会

作者：朱平李佳妮李小康庄建广东省心血管病研究所广东省人民医院广东省医学科学院日本国立成育医疗研究中心放射线安全与免疫耐受研究部

Objective:The development of an animal model bearing definite antigens is important to facilitate the evaluation and modulation of specific allo-antigen responses after ***:In the present study,heterotopic cardiac transplantation was performed from F344/EGFPTg and F344/HLAB27Tg rats to F344 ***:The F344 recipients accepted the F344/EGFPTg transplants,whereas they rejected the cardiac tissue from the F344/HLA-B27Tg rats by 39.4±6.5 days,due to high production of anti-HLA-B27 IgM-and IgG-specific *** addition,immunization of F344 rats with skin grafts from F344/HLA-B27Tg rats resulted in robust production of antiHLA-B27 IgM and IgG antibodies and accelerated the rejection of a secondary cardiac allograft(7.4±1.9 days).Of interest,the F344 recipients rejected cardiac grafts from double transgenic F344/HLA-B27&EGFPTg rats within 9.0±3.2days,and this was associated with a significant increase in the infiltration of lymphocytes by day 7,suggesting a role for cellular immune *** acid(EPA),one of the w-3 polyunsaturated fatty acids in fish oil,could attenuate the production of anti-HLA IgG antibodies and B-cell proliferation,significantly prolonging double transgenic F344HLA-B27&EGFPTg to F344 rat cardiac allograft survival(36.1±13.6 days).Moreover,the mRNA expression in the grafts was assessed by qRT-PCR,revealing an increase in the expression of the HO-1,IL-10,TGF-b,IDO,and Foxp3genes in the EPA-treated ***:Our data indicate that HLA-B27 and/or GFP transgenic proteins could establish a unique animal transplantation model to clarify the mechanism of allogeneic cellular and humoral immune response,in which the transplant antigens are specifically *** also demonstrated that EPA was effective in the treatment of rat cardiac allograft rejection and may develop a novel immunomodulatory strategies for organ transplantation.

关键词： Allograft GFP HLA-B27 Regulatory T cell Tolerance transgenic rat

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Retake the Center Stage-New Development of rat Genetics

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Journal of Genetics and Genomics 2012年第6期39卷 261-268页

作者： Sushuang Zheng Kindiya Geghman Sushila Shenoy Chenjian Li Departntent of Neunrology. Friedman Brain Institute Mt. Sinai School of Medicine Box 1137 New York NY 10029 USA College of Life Sciences Peking Univetsitv Beijing 100871 China

The rat is a powerful model for the study of human physiology and diseases, and is prefened by physiologists, neuroscientists and toxicologists. However, the lack of robust genetic modification tools has severely limited the generation of rat genetic models over the last two decades. In the last few years, several gene-targeting strategies have been developed in rats using N-ethyl-N-nitrosourea （ENU）, transposons, zinc-finger nucleases （ZFNs）, bacterial a11ificial chromosome （BAC） mediated transgenesis, and recently established rat embryonic stem （ES） cells. The development and improvement of these approaches to genetic manipulation have created a bright future lbr the use of genetic rat models in investigations of gene function and human diseases. Here, we summarize the strategies used for rat genetic manipulation in current research. We also discuss BAC transgenesis as a potential tool in rat transgenic models.

关键词： rat model BAC Knockout rat transgenic rat Mutagenesis

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Schizophrenia-related behavioral phenotypes in transgenic rats expressing elevated forebrain dopamine D5 receptors

Schizophrenia-related behavioral phenotypes in transgenic ra...

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中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Hui-Kun WANG~1,Ting-He HU~2,Xue-Chu ZHEN~(1,*) 1 State Key Laboratory of Drug Research,Shanghai Institute of Materia Medica,Shanghai Institutes of Biological Sciences,Chinese Academy of Sciences,Shanghai 201203,China 2 Shanghai Institute of Brain Functional Genomics,East China Normal University,Shanghai 200062,China

＜正＞Dysfunction of dopamine system in central nervous system is one of the key mechanisms in the pathological processing of *** the dominant roles of D2 receptor hyperfunction associated with positive symptoms of schizophrenia,the abnormal activities of D1-like receptor in the pathogenesis of negative symptoms and cognitive deficits in the disease have attracted great attention recently.D1-like receptors are comprised of D1 and D5 subtypes. Since no pharmacological tool is available to distinguish these two subtypes,the relative contribution between D1 and D5 receptors in schizophrenia remains largely *** taking the advantage of alpha-CaMKII-D5 transgenic rats,in which expression of D5 receptor in forebrain is selectively elevated,we explored the potential roles of D5 receptor in the schizophrenia-related *** transgenic rats exhibited similar basal activity of locomotion with their wild type （WT） *** acute injection of phencyclidine（PCP）,an NMD A receptor antagonist which can induce psychotic symptoms,male D5 transgenic rats showed comparably increase in total and central area locomotion with WT rats,while female D5 transgenic rats showed a longer latency to achieve enhanced central area locomotion than WT ***, male D5 transgenic rats exhibited normal sensory-motor gating properties in either basal condition or in response to PCP ***,compared to WT control,female D5 transgenic rats were more resistant to PCP-induced suppression of sensory-motor gating at the dosage of 3 mg/*** T-maze delayed alternation task,male D5 transgenic rats showed higher working memory capacity at the retention interval of 80s under basal ***,working memory of male D5 transgenic rats were more susceptible to be disrupted by psychostimulant *** summary,our present data demonstrated that alteration of D5 receptor activity may take a part in the pathogenesis of impaired working memory in schizop

关键词： schizophrenia dopamine D5 receptor transgenic rat behavior

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