检索结果-南通市图书馆

Stable Skin-specific Overexpression of Human CTLA4-Ig in transgenic mice through Seven Generations

Acta Biochimica et Biophysica Sinica 2006年第3期38卷 171-178页

作者： Yong WANG~1 Yong NI~(1,3) Hong WEI~1* Feng-Chao WANG~2 Liang-Peng GE~1 Xiang GAO~41 Department of Animal Science,College of Basic Medicine,Third Military Medical University,Chongqing 400038,China 2 Institute of Combined Injury,College of Military Preventive Medicine,Third Military Medical University,Chongqing 400038,China 3 School of Life Sciences,Southwest University,Chongqing 400715,China 4 Animal Model Research Institute,Nanjing University,Nanjing 210093,China Department of Animal Science College of Basic Medicine Third Military Medical UniversityChongqing 400038 China School of Life Sciences Southwest UniversityChongqing 400715 China Institute of Combined Injury College of Military Preventive Medicine Third Military Medical UniversityChongqing 400038 China Animal Model Research Institute Nanjing UniversityNanjing 210093 China

Skin graft rejection is a typical cellular immune response,mainly mediated by T *** lymphocyte associated antigen 4-immunoglobin (CTLA4-Ig) extends graft survival by blocking the T cellco-stimulation pathway and inhibiting T cell *** investigate the efficacy of CTLA4-Ig in prolong-ing skin graft survival,human CTLA4-Ig (hCTLA4-Ig) was engineered to overexpress in mouse skin bytransgenesis using the K14 *** transcription-polymerase chain reaction (RT-PCR) and West-ern blot assay indicated that the expression of CTLA4-Ig remained skin-specific and relatively constantcompared to the internal control protein,AKT,through seven *** presence and concentrationof the hCTLA4-Ig protein in transgenic mouse sera was determined by enzyme-linked immunosorbent assay(ELISA),and the results indicated that the serum CTLA4-Ig concentration also remained constant *** of transgenic mouse skins grafted onto rat wounds was remarkably prolonged com-pared to that of wild-type skins from the same mouse strain,and remained comparable among all *** suggested that the bioactive hCTLA4-Ig protein was stably expressed in transgenical micethrough at least seven generations,which was consistent with the stable skin-specific CTLA4-Ig *** results demonstrated that the transgenic expression of hCTLA4-Ig in skin driven by the K14 promoterremained constant through generations,and a transgenic line can be established to provide transgenic skinwith extended survival reproducibly.

关键词： cytotoxic T lymphocyte antigen 4-Ig transgenic mice skin K14 promoter

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Production of transgenic mice by Type-A Spermatogonia-Mediated Gene Transfer

引用

Agricultural Sciences in China 2011年第3期10卷 431-437页

作者： JU Hui-ming BAI Li-jing REN Hong-yan MU Yu-lian YANG Shu-lin LI Kui Key Laboratory for Farm Animal Genetic Resources and Utilization Ministry of Agriculture~Institute of Animal Science Chinese Academyof Agricultural Sciences Beijing 100193 P.R. China College of Veterinary Medicine Yangzhou University Yangzhou 225009 P.R. China

Type-A spermatogonia first appear at between 3-7 d postnatally in mice and are the only immortalized diploid cells that reproduce in adulthood in these animals. In our current study, we explored the feasibility of producing stable transgenic mice using these cells. Enhanced pEGFP-N1 plasmids were suspended in ExGen500 transfection reagent and injected at different angles into the testes of 7-d-old male ICR mice. The resulting type-A spermatogonia-mediated gene transfer （TASMGT） mice were then mated with normal females at different stages of sexual maturity （6, 12, and 24 wk）. The integration and expression of the introduced EGFP gene was evaluated in the F1 transgenic offspring by PCR and Southern blotting analysis. The foreign gene integration rates for a low-dose group （15 μL gene suspension injected into each testis） and a high-dose group （30 μL suspensions injected） at the three stages of female sexual maturity tested were 11.76% （2/17）, 14.29% （3/21）, and 11.11% （2/18）, and 5% （1/20）, 5.56% （1/18）, and 0 （0/17）, respectively. The average integration rates for these two dose groups were 12.5% （7/56） and 3.64% （2/55）, respectively, which was a significant difference （P0.05）. Semi-quantitative RT-PCR analysis further showed that the introduced GFP gene was expressed in 3/9 integration mice. In addition, GFP expression was observed in the sperm cells from the TASMGT mice, and also in the embryos and F2 pups from the F1 generation transgenic mice. Hence, although the foreign gene integration rate for TASMGT is not high and the transgenic offspring show as yet unexplained defects, our results indicate that this method is a potentially feasible and reproducible new approach to creating transgenic mice.

关键词： type-A spermatogonia transgenic mice enhanced green fluorescent protein type-A spermatogonia-mediated gene transfer

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Characterization of transgenic mice expressing EGFP under the control of the monkey 20α-hydroxysteroid dehydrogenase promoter

引用

Science China(Life Sciences) 2018年第8期61卷 966-974页

作者： Chae-Won Park Seong-Keun Jeong Tsevelmaa Nanjidsuren Munkhzaya Byambaragchaa Myung-Hwa Kang Bo-Woong Sim Kwan-Sik Min Animal Biotechnology Graduate School of Future Convergence Technology Department of Animal Life Science Institute of Genetic Engineering Hankyong National University Department of Food Science and Nutrition Hoseo University National Primate Research Center Korea Research Institute of Bioscience and Biotechnology

To directly assess the molecular function of the monkey 20α-hydroxysteroid dehydrogenase （20α-HSD） promoter, we generated transgenic mice （tg） expressing enhanced green fluorescent protein （EGFP） under control of this promoter. We demonstrated that prostaglandin F2α induced 20α-HSD promoter activity in CHO cells in a dose-dependent manner. Furthermore, forskolin treatment markedly reduced 20α-HSD promoter activity, and prolactin exhibited weak inhibitory activity. The transgenic mouse obtained one positive founder male. The transgene was propagated in 10 successive generations without any notable defects to the progeny. EGFP and 20α-HSD in the tg mice were colocalized in the luteal cells of the ovary during late pregnancy. Strong EGFP and 20et-HSD protein signals were also detected in the adult testis. Immtmohistochemical analysis revealed high EGFP levels in the seminiferous epithelium, whereas 20α-HSD was expressed in the seminiferous tubules. Our data suggest that the ovaries in monkey and mouse exhibit similar expression patterns of 20α-HSD during pregnancy. However, the expression pattern of EGFP in tg mice testis slightly differed from that of the endogenous 20α-HSD. Further investigation is required to elucidate the functional mechanisms underlying regulation of the monkey 20α-HSD promoter in the tg mice.

关键词： transgenic mice EGFP monkey 20α-HSD promoter

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Inducible overexpression of porcine homeobox A10 in the endometrium of transgenic mice

引用

Journal of Integrative Agriculture 2016年第6期15卷 1338-1344页

作者： LIN Rui-yi WU Di ZHAO Chang-zhi CHEN Shang-shang XIAO Qian LI Xin-yun ZHAO Shu-hong Key Lab of Agricultural Animal Genetics Breeding and Reproduction Ministry of Education/The Cooperative Innovation Center for Sustainable Pig Production Huazhong Agricultural University Wuhan 430070 P.R China College of Animal Science and Technology Northeast Agricultural University Harbin 150030 P.R.China

Homeobox A10 （HOXA 10） is a well-known transcription factor that plays an important role in directing endometrial differ- entiation and establishing the conditions required for implantation. Interestingly, the expression level of HOXAIO may be associated with litter size. To study the effects of the porcine HOXAIO promoter fragment on the expression of HOXAIO gene in vivo, we generated a transgenic mouse model using pronuclear microinjection, and measured the expression of HOXAIO in the endometrium. There was no difference in the expression level of HOXAIO between transgenic and wild- type mice in the absence of hormone stimulation. However, following treatment with progesterone and estradiol benzoate, the expression level of HOXAIO was significantly increased in transgenic mice compared with that of wild-type mice. Fur- thermore, the litter size of transgenic females was larger than that of wild-type females （7.02±1.73 vs. 6.48＋1.85; P=0.14）. Moreover, the difference of litter size was greater in the later parities （7.33±1.62 vs. 6.37±2.02; P=0.08） compared with the first parity （6.76±1.81 vs. 6.61v1.67; P=0.77） between transgenic and wild-type mice. Therefore, our transgenic mouse model provides exciting insights regarding the actions of HOXAIO and its hormone-inducible promoter in vivo. The present study offers valuable proof of principle to develop transgenic pigs with a hormone-inducible promoter regulating HOXAIO to alter litter size.

关键词： porcine HOXA 10 transgenic mice endometrium

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The Establishment of Double-transgenic mice that Co-Express the appA and MxA Genes Mediated by Type A Spermatogonia In vivo

引用

Journal of Integrative Agriculture 2014年第12期13卷 2741-2749页

作者： BAI Li-jing JU Hui-ming MU Yu-lian YANG Shu-lin REN Hong-yan AO Hong WANG Chu-duan LI Kui State Key Laboratory for Animal Nutrition Ministry of Agriculture/Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation Ministry of Agriculture/Institute of Animal Science Chinese Academy of Agricultural Sciences College of Animal Science China Agricultural University College of Veterinary Medicine Yangzhou University

Type A spermatogonial stem cells are the only immortal diploid cells in the postnatal animal that undergo self-renewal through the lifetime of an animal and transmit genes to subsequent generations. In this paper, the generation and characterization of double-transgenic mice co-expressing the Escherichia coli appA gene and human MxA gene generated via the in vivo transfection of type A spermatogonial cells were reported for the ifrst time. The dicistronic expression vector pcDNA-appA-MxA（AMP） and ExGen500 transfection reagent were injected into the testicular tissue of 7-d-old male ICR mice. The mice that underwent testis-mediated gene transfer were mated with wild-type female mice, and the integration and expression of the foreign genes in the offspring were evaluated. transgenic mice that co-expressed appA and MxA showed a gene integration rate of 8.89%（16/180）. The transgenic mice were environmentally friendly, as the amount of phosphorous remaining in the manure was reduced by as much as 11.1%by the appA gene （P〈0.05）;these animals also exhibited a strong anti-viral phenotype.

关键词： type A spermatogonial stem cells dicistronic expression vector co-expression transgenic mice

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Establishment of Hamster-and Human-PRNP transgenic mice

引用

Biomedical and Environmental Sciences 2011年第6期24卷 608-616页

作者： GONG Han Shi TIAN Chan ZHANG Bao Yun WANG Zhao Yun XIE Wu Ling JING Yuan Yuan GAO Chen JIANG Hui Ying SHI Qi LIU Yong DONG Xiao Ping School of Medicine Xi＇an Jiao Tong University Xi＇an 710061 Shaanxi China State Key Laboratory for Infectious Disease Prevention and Control National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 102206 China

Objective To create transgenic mice expressing hamster- and human-PRNP as a model tor understanding the physiological function and pathology of prion protein （PrP）, as well as the mechanism of cross-species transmission of transmissible spongiform encephalopathies （TSEs）. Methods Hamster and human-PRNP transgenic mice were established by conventional methods. The copy number of integrated PRNP in various mouse lines was mapped by real-time PCR. PRNP mRNA and protein levels were determined by semi-quantitative RT-PCR, real-time RT-PCR, and western blot analysis. Histological analyses of transgenic mice were performed by hematoxylin and eosin （H ＆ E） staining and immunohistochemical （IHC） methods. Results Integrated PRNP copy number in various mouse lines was 53 （Tg-haPrP1）, 18 （Tg-huPrP1）, 3 （Tg-huPrP2）, and 16 （Tg-huPrP5）, respectively. Exogenous PrPs were expressed at both the transcriptional and translational level. Histological assays did not detect any abnormalities in brain or other organs. Conclusion We have established one hamster-PRNP transgenic mouse line and three human-PRNP transgenic mouse lines. These four transgenic mouse lines provide ideal models for additional research.

关键词： PrP PRNP transgenic mice Copy number

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Antiviral treatment of hepatitis B virus-transgenic mice by a marine organism, Styela plicata

引用

World Journal of Gastroenterology 2006年第25期12卷 4038-4043页

作者： Rui Wang Zhen-Lan Du Wen-Jun Duan Xin Zhang Fan-Lin Zeng Xin-Xiang Wan Research Center for Pharmaceutics Southern Medical University Guangzhou 510315 Guangdong Province China Hematopoietic Stem Cell Transplantation 307 Hospital Academy of Military Medical Sciences Beijing 100039 China

AIM： To evaluate the antiviral effect of the effective ingredient of Styela plicata in a murine model of hepatitis B virus carrier. METHODS： HBV-transgenic mice were divided into 3 groups （control group, lamivudine treatment group and the effective ingredient of Styela plicata treatment group） and assigned to receive normal diet, lamivudine or the effective ingredient of Styela plicata for consecutive weeks. Serum hepatitis B surface antigen was detected by enzyme-linked immunosorbent assay （ELISA） method. Serum HBV DNA was detected by real-time polymerase chain reaction （RT-PCR）. Serum T helper （h） 1 cytokine interleukin （IL）-2 and Th2 cytokine IL-6 were detected by the quantitative sandwich enzyme immunoassay technique. Another group of HBV-transgenic mice was assigned to receive the effective ingredient of Styela plicata for consecutive weeks. The histology of liver tissue was evaluated before and after treatment. RESULTS： Twelve weeks after starting the therapy, serum hepatitis B surface antigen was significantly lowered in Styela plicata -treated mice and lamivudine-treated mice compared with the mice receiving normal diet （F12wk = 88.81, P12wk = 0.000 〈 0.01）. Serum HBV DNA was significantly lowered in Styela plicata -treated mice and lamivudine-treated mice compared with the mice receiving normal diet （F12wk = 20.71, P12wk = 0.000 〈 0.01）. However, like lamivudine, the effective ingredient of Styela plicata could not inhibit the replication of HBV completely. A rebound phenomenon of hepatitis B surface antigen and HBV DNA in sera could be found 4 wk after withdrawal of medication. Eight weeks after starting the therapy, serum levels before and after Styela plicata treatment of IL-2 were 2.41 ± 0.38 and 10.56 ± 0.78 ng/L, respectively （t8wk = -16.51, P8wk = 0.000 〈 0.01）. Compared with the serum levels of IL-2 in the normal diet-treated mice （2.48 ± 0.17 ng/L; t8wk = 13.23, P8wk = 0.000 〈 0.01）. Serum levels before and after Styela plicata treatment of IL-

关键词： Styela plicata Hepatitis B virus transgenic mice RT-PCR Chronic hepatitis B

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Overexpression of γ-aminobutyric acid transporter subtype I leads to susceptibility to Kainic acid-induced seizure in transgenic mice

引用

Cell Research 2001年第1期11卷 61-67页

作者： MA YINGHUA, JIA HUA HU, WEN JUAN ZHAO, JIAN FEI, YUN YU, XIAO GANG ZHOU, ZHEN TONG MEI, LI HE GUO (Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China) (Shanghai Inst Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China Shanghai Institute of Physiology Chinese Academy of Sciences Shanghai China

γ-aminobutyric acid (GABA) is the principal inhibitory neurotransmitter, and the GABAergic synaptic transmission is normally terminated by the rapid uptake through GABA transporters. With transgenic mice ubiquitously overexpressing GABA transporter subtype I (GAT1), the present study explored the pathophysiological role of GAT1 in epileptogenesis. Though displaying no spontaneous seizure activity, these mice exhibit altered electroencephalographic patterns and increased susceptibility to seizure induced by kainic acid. In addition, the GABAA receptor and glutamate transporters are up-regulated in transgenic mice, which perhaps reflects a compensatory or corrective change to the elevated level of GAT1. These preliminary findings support the hypothesis that excitatory and inhibitory neurotransmission, and seizure susceptibility can be altered by neurotransmitter transporters.

关键词： γ-aminobutyric acid transporter seizure susceptibility kainic acid electroencephalography transgenic mice

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

MicroRNA Profiling of transgenic mice with Myocardial Overexpression of Nucleolin

引用

Chinese Medical Journal 2018年第3期131卷 339-346页

作者： Qing-Lan Lyu Bi-Mei Jiang Bin Zhou Li Sun Zhong-Yi Tong Yuan-Bin Li Yu-Ting Tang Hui Sun Mei-Dong Liu Xian-Zhong Xiao Department of Pathophysiology Xiangya School of Basic Medical Sciences Central South University Changsha Hunan 410078 China Department of Rheumatology Xiangya Hospital Central South University Changsha Hunan 410008 China Department of Pathology The Second Xiangya Hospital Central South University Changsha Hunan 410000 China

Background：Nucleolin （NCL） is the most abundant RNA-binding protein in the cell nucleolus and plays an important role in chromatin stability,ribosome assembly,ribosomal RNA maturation,ribosomal DNA transcription,nucleocytoplasmic transport,and regulation of RNA stability and translation *** addition to its anti-apoptotic properties,the underlying mechanisms associated with NCL-related roles in different cellular processes remain *** this study,the effect of NCL on microRNA （miRNA） expression was evaluated by generating transgenic mice with myocardial overexpression of NCL and by analyzing microarrays of mature and precursor miRNAs from ***：Using microinjection ofalpha-MyHc clone 26-NCL plasmids,we generated transgenic mice with myocardial overexpression of NCL firstly,and then mature and precursor miRNAs expression profiles were analyzed in NCL transgenic mice （n =3） and wild-type （WT） mice （n =3） by miRNA *** Package for the Social Sciences version 16.0 software （SPSS,Inc.,Chicago,IL,USA） was used to perform Student＇s t-test,and statistical significance was determined at P 〈 ***：Several miRNAs were found to be differentially expressed,of which 11 were upregulated and 4 were downregulated in transgenic mice with myocardial overexpression of NCL compared to those in WT *** differentially expressed miRNAs were subsequently confirmed and quantified by real-time quantitative reverse transcription-polymerase chain *** analysis was used for the prediction of miRNA ***,in vitro experiments showed that NCL regulated miR-21 expression following hydrogen peroxide ***：Myocardial-protection mechanisms exerted by NCL might be mediated by the miRNAs identified in this study.

关键词： MicroRNA microarray Myocardium Nucleolin transgenic mice

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Generation of the regulatory protein rtTA transgenic mice

引用

World Journal of Gastroenterology 2005年第19期11卷 2885-2891页

作者： KangXu Xin-YanDeng YingYue Zhong-MinGuo BingHuang XunHong DongXiao Xi-GuChen CenterofExperimentalAnimals SunYat-Sen(Zhongshan)UniversityGuangzhou510080GuangdongProvinceChina

AIM: To translate Tet-on system into a conditional mouse model, in which hepatitis B or C virus (HBV or HCV) gene could be spatiotemporally expressed to overcome 'immune tolerance' formed during the embryonic development and 'immune escape' against hepatitis virus antigen(s), an effector mouse, carrying the reverse tetracycline-responsive transcriptional activator (rtTA) gene under the tight control of liver-specific human apoE promoter, is required to be generated. METHODS: To address this end, rtTA fragment amplified by PCR was effectively inserted into the vector of pLiv.7 containing apoE promoter to create the rtTA expressing vector, I.e., pApoE-rtTA. ApoE-rtTA transgenic fragment (-6.9 kb) released from pApoE-rtTA was transferred into mice by pronucleus injection, followed by obtaining one transgene (+) founder animal from microinjection through PCR and Southern blot ***: rtTA transgene which could be transmitted to subsequent generation (F1) derived from founder was expressed in a liver-specific fashion. CONCLUSION: Taken together, these findings demonstrate that rtTA transgenic mice, in which rtTA expression is appropriately targeted to the murine liver, are successfully produced, which lays a solid foundation to 'off-on-off' regulate expression of target gene (s) (e.g., HBV and/or HCV) in transgenic mice mediated by Tet-on system.

关键词： Hepatitis virus Tet-on system transgenic mice Liver-specific human apoE promoter

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：