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Creation of transgenic banana. Expressing Human Lysozyme Gene for Panama Wilt Resistance

Journal of Integrative Plant Biology 2005年第8期47卷 971-977页

作者： Xin-Wu PEI Shi-Kai CHEN Rui-Ming WEN Shang YE Jia-Qin HUANG Yong-Qiang ZHANG Bing-Shan WANG Zhi-Xing WANG Shi-Rong JIA Biotechnology Institute Chinese Academy of Agricultural Sciences Beijing 100081 China Xinyi Institute of Agricultural Science and Technology Xinyi Guangdong 523530 China

Human lysozyme （HL） inhibits Fusarium oxysporum （FocR4） growth in vitro. To obtain transgenic banana. （Musa spp.） that are resistant to Panama wilt （F. oxysporum）, we introduced an HL gene that is driven by a constitutive cauliflower mosaic virus 35S promoter into the banana.via Agrobacteriummediated transformation. PCR confirmed that 51 transgenic plants were obtained. The development of Panama wilt symptoms were examined after the plants had been grown in pots. The non-transgenic plants developed typical fusarium symptoms 60 d after FocR4 inoculation, whereas 24 of 51 transgenic plants remained healthy. The transgenic banana.plants that showed resistance to FocR4 in the pots were then planted in a field that was heavily infected with FocR4 for further investigation. Eleven of 24 plants devel- oped symptoms before bud emergence; another 11 plants showed symptoms after bud emergence and the remaining two plants, H-67 and H-144, remained healthy and were able to fruit. Northern blotting analysis demonstrated that H-67 and H-144, bearing the strongest resistance to Panama wilt, had the highest level of HL expression and that the expression of HL was well correlated with the FocR4 resistance of transgenic plants. We conclude that Agrobacterium-mediated transformation, with the assistance of particle bombardment, is a powerful approach for banana.transformation and that a transgenic HL gene can cause resistance of the crop to FocR4 in the field.

关键词： Agrobacterium-mediated transformation Fusarium oxysporum human lysozyme Panamawilt disease particle bombardment transgenic banana.

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