检索结果-南通市图书馆

A mitochondrial phosphate transporter,McPht gene,confers an acclimation regulation of the transgenic rice to phosphorus deficiency

引用

Journal of Integrative Agriculture 2018年第9期17卷 1932-1945页

作者： HAN Jiao YU Guo-hong WANG Li LI Wei HE Rui WANG Bing HUANG Sheng-cai CHENG Xian-guo College of Life Science Shanxi Normal University Institute of Agricultural Resources and Regional Planning Chinese Academy of Agricultural Sciences College of Land and Environment Shenyang Agricultural University

Phosphate transporters play an important role in promoting the uptake and transport of phosphate in plants. In this study, the McPht gene from the Mesembryanthemum crystallinum, a mitochondrial phosphate transporter, was isolated and constructed onto a constitutive expression vector carrying 35S：：GFP, and the recombinant constructs were transferred into Oryza sativajaponica L. cv. Kitaake to investigate the regulatory role of the McPhtgene under phosphorus deficiency. The McPhtgene encodes a protein of 357 amino acids with six transmembrane domains and is located to the mitochondria, and the mRNAtranscripts of the McPht gene are highly accumulated in the shoots of M. crystallinum in response to phosphorus deficiency. However, more mRNA transcripts of the McPht gene were accumulated in the roots of the transgenic rice under phosphorus deficiency. Measurements showed that the transgenic rice demonstrated an enhanced promotion in the root development, the root activities, and phosphate uptake under phosphorus deficiency. Transcriptome sequencing showed that the transgenic rice exhibited total of 198 differentially expressed genes. Of these, total of 154 differentially expressed genes were up-regulated and total 44 genes were down-regulated comparing to the wild type in response to phosphorus deficiency. The selective six genes of the up-regulated differentially expressed genes showed an enhanced increase in mRNA transcripts in response to phosphorus deficiency, however, the transcripts of the mitochondrial carrier protein transporter in rice, a homologous gene of the McPht, in both the transgenic line and the wild type had no obvious differences. Functional enrichment analyses revealed that the most of the up-regulated genes are involved in the cytoplasmic membrane-bounded vesicle, and most of the down-regulated genes are involved in the mitochondrion and cytoplasmic membrane-bounded vesicle. The differentially expressed genes were highly enriched in plant secondary me

关键词： McPht gene phosphorus deficiency phosphate transporter transcriptome sequencing transgenic rice

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

CO2 Exchange and Chlorophyll Fluorescence of Phosphoenolpyruvate Carboxylase transgenic rice Pollen Lines

引用

Journal of Integrative Plant Biology 2006年第12期48卷 1431-1438页

作者： Li-Li Ling Hong-Hui Lin Ben-Hua Ji De-Mao Jiao Key Laboratory of Bio-resources and Eco-environment（Ministry of Education） College of Life Sciences Sichuan University Chengdu 610064 China Institute of Life Sciences Nantong University Nantong 510061 China Jiangsu Academy of Agricultural Sciences 84-401 Nanjing 210014 China

To elucidate the photosynthetic physiological characteristics and the physiological inherited traits of rice （Oryza sativa L.） hybrids and their parents, physiological indices of photosynthetic CO2 exchange and chlorophyll fluorescence parameters were measured in leaves of the maize phosphoenolpyruvate carboxylase （PEPC） transgenic rice as the male parent, sp. japonica rice cv. 9516 as the female parent, and the stable JAAS45 pollen line. The results revealed that the PEPC gene could be stably inherited and trans- ferred from the male parent to the JAAS45 pollen line. Moreover, the JAAS45 pollen line exhibited high levels of PEPC activity, manifesting higher saturated photosynthetic rates, photosynthetic apparent quantum yield （AQY）, photochemical efficiency of photosystem II and photochemical and non-photochemical quenching, which indicated that the JAAS45 pollen line has a high tolerance to photo-inhibition/photooxidation under strong light and high temperature. Furthermore, JAAS45 was confirmed to still be a C3 plant by δ^13C carbon isotope determination and was demonstrated to have a limited photosynthetic C4 microcycle by feeding with exogenous C4 primary products, such as oxaloacetate or malate, or phosphoenolpyruvate. The present study explains the physiological inherited properties of PEPC transgenic rice and provides an expectation for the integration of traditional breeding and biological technology.

关键词： anther culture phosphoenolpyruvate carboxylase (PEPC) physiological inherited trait rice hybrid transgenic rice

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Preparative Purification and Bioassay of Bt Toxin from Cry1Ab transgenic rice

引用

Chemical Research in Chinese Universities 2004年第5期20卷 579-583页

作者： WUJian-min YEQin-fu DepartmentofChemistry FacultyofScienceZhejiangUniversityHangzhou310027P.R.China InstituteofNuclear-AgriculturalScience ZhejiangUniversityHangzhou310029P.R.China

A method of extracting and purifying Cry1Ab protein(Bt toxin) from Cry1Ab transgenic rice was established. Most of the Bt toxin present in the tissue of Cry1Ab transgenic rice was extracted effectively with a solution of 50 mmol/LNa_2CO_3 and NaHCO_3. The crude protein containing Bt toxin was obtained after the pretreatment of Cry1Ab transgenic rice with ultra-filtration, ammonium sulfate precipitation and centrifugation. The dialysed crude protein was futher separated on DEAE Sephadex A-50 columns and Sephadex G-150 columns. The protein bound on DEAE Sephadex A-50 gel was eluted with buffer solution B(10 mmol/L tris-HCl buffer+1.0 mmol/L EDTA, pH=8.0) mixed with 0.1, 0.3, 0.5 and 0.8 mol/L NaCl in a discontinuous gradient elution mode. The peak of the Bt toxin eluted from the columns was identified by ELISA and bioassayed with larvae of tobacco hornworms and silkworms. The purity and the bioactivity of the Bt toxin were determined by means of SDS-PAGE and larvicidal assay, respectively. The purity of the Bt toxin obtained by this method is high, and its insecticidal activity is retained after the toxin is purified.

关键词： transgenic rice Bt toxin Purification Bioassay

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Western blot detection of PMI protein in transgenic rice

引用

Journal of Integrative Agriculture 2016年第4期15卷 726-734页

作者： RONG Rui-juan WU Peng-cheng LAN Jin-ping WEI Han-fu WEI Jian CHEN Hao SHI Jia-nan HAO Yu-jie LIU Li-juan DOU Shi-juan LI Li-yun WU Lin LIU Si-qi YIN Chang-cheng LIU Guo-zhen College of Life Sciences Hebei Agricultural University Baoding 071001 P.R. China Beijing Protein Innovation Co. Ltd. Beijing 101318 P.R.China Beijing Institute of Genomics Chinese Academy of Sciences Beijing 100101 P.R. China

Phosphomannose isomerase （PMI） encoding gene manA is a desirable selective marker in transgenic research. Under- standing of its expression patterns in transgenic plant and establishing highly sensitive detection method based on immunoassay have great impacts on the application of PMI. In this study, PMI-specific monoclonal antibodies were generated using recombinant protein as immunogen, and could be used in Western blot to detect as little as 0.5 ng His-tagged PMI protein or rice expressed PMI protein in sample accounted for 0.4% of single rice grain （about 0.08 mg）. PMI protein driven by CaMV-35S promoter was detected in dozens of tested tissues, including root, stem, leaf, panicle, and seed at all developmental stages during rice growing, and PMI protein accounted for about 0.036% of total protein in the leaves at seedling stage. The established method potentially can be used to monitor PMI protein in rice grains.

关键词： transgenic rice protein expression CaMV-35S promoter phosphomannose isomerase (PMI) Western blot

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Thellungiella halophila ThPIP1 gene enhances the tolerance of the transgenic rice to salt stress

引用

Journal of Integrative Agriculture 2015年第10期14卷 1911-1922页

作者： QIANG Xiao-jing YU Guo-hong JIANG Lin-lin SUN Lin-lin ZHANG Shu-hui LI Wei CHENG Xian-guo Key Lab of Plant Nutrition and Fertilizers Ministry of Agriculture/Institute of Agricultural Resources and Regional PlanningChinese Academy of Agricultural Sciences College of Land and Environment Shenyang Agricultural University

Aquaporin proteins were demonstrated to play an important regulatory role in transporting water and other small molecules. To better understand physiological functions of aquaporins in extremophile plants, a novel ThPIP1 gene from the Thellungiella halophila was isolated and functionally characterized in the transgenic rice. Data showed that the ThPIP1 protein encoded 284 amino acids, and was identified to be located on the plasma membrane. The expression of ThPIP1 gene in the shoots and roots of T. halophila seedlings were induced by high salinity. The transgenic rice overexpressing ThPIP1 gene significantly increased plants tolerance to salt stress through the pathway regulating the osmotic potentials, accumulation of organic small molecules substances and the ratio of K＋/Na＋ in the plant cells. Moreover, split-ubiquitin yeast two-hybrid assay showed that Th PIP1 protein specifically interacted with ThPIP2 and a non-specific lipid-transfer protein 2, suggesting that ThPIP1 probably play a key role in responding to the reactions of multiple external stimulus and in participating in different physiological processes of plants exposed to salt stress.

关键词： ThPIP1 transgenic rice salt stress protein interaction Thellungiella halophila

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

RNAi-mediated transgenic rice resistance to rice stripe virus

引用

Journal of Integrative Agriculture 2016年第11期15卷 2539-2549页

作者： LI Li GUO Cheng WANG Biao ZHOU Tong LEI Yang DAI Yu-hua HE Wen LIANG Chun WANG Xi-feng State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural Sciences Department of Biology Miami University Institute of Plant Protection Jiangsu Academy of Agricultural Sciences Department of Computer Science and Software Engineering Miami University

rice stripe virus（RSV） often causes severe rice yield loss in temperate regions of East Asia. Although the correlation of small interfering RNAs（si RNAs） with transgenic virus resistance of plants using RNA interference（RNAi） is known for decades, no systematical research has been done on the profiling of si RNAs from a genomic scale. Our research is aiming to systematically study the RNAi impact in RSV-resistant transgenic rice, which was generated by introducing an inverted repeat construct that targets RSV nucleocapsid protein（NCP） gene. In this paper, three independent RSV-retsistant transgenic rice lines were generated, their stable integration of the T-DNA fragment and the expression of si RNAs were confirmed by Southern blotting and Northern blotting analyses, and the majority of si RNAs were in lengths of 21, 22, and 24 nucleotides（nt）, which have validated a connection between the presence of the RSV NCP homologous si RNAs and the RSV resistance in those transgenic rice lines. In one of these transgenic lines（T4-B1）, the T-DNA fragment was found to have been inserted at chromosome 1 of the rice genome, substituting the rice genome fragment from 32 158 773 to 32 158 787 nt. Bioinformatics analysis of small RNA-Seq data on the T4-B1 line also confirmed the large population of NCP-derived si RNAs in transgenic plants, and the RSV-infected library（T4-B1-V） possessed more si RNAs than its mock inoculated libraries（T4-B1-VF）, these results indicating the inverted repeat construct and RSV could introduce abundance of si RNAs in transgenic rice. Moreover, a varied expression level of specific si RNAs was found among different segments of the NCP gene template, about 47% of NCP-derived si RNAs reads aligned with the fragment from 594 to 832 nt（239 nt in length） in NCP gene（969 nt in length） in the T4-B1-V, indicating a potential usage of hotspot regions for RNAi silencing in future research. In conclusion, as the first study to address the si RNA profile in RSV-resi

关键词： rice stripe virus(RSV) transgenic rice deep sequencing siRNA resistance

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Different Responses of Plant Growth and Antioxidant System to the Combination of Cadmium and Heat Stress in transgenic and Non-transgenic rice

引用

Journal of Integrative Plant Biology 2009年第10期51卷 942-950页

作者： Feng-Yun Zhao Wen Liu Shi-Yong Zhang College of Life Sciences Shandong University of Technology Zibo 255049 China Institute of rice Jining 272077 China

A comparative study of just cadmium （Cd） or heat and their combination treatments on some physiological parameters and the antioxidant systems in transgenic rice （Oryza sativa L. cv. Zhonghua No.11） carrying glutathione-S-transferase （GST, EC. 2.***.1.18） and catalasel （CAT1, EC. 1.***.1.6） and non-transgenics was conducted. The results revealed improved resistance in the transgenics to Cd and the combined Cd and heat stress than non-transgenics. Data showed that the activities of CAT, GST, superoxide dismutase （EC.1.***.1.1） and all components of the ascorbate-glutatbione cycle measured in the stressed transgenics shoots are significantly different from those of non-transgenics. Results indicated that co-expression of GST and CAT1 had an important effect on the antioxidant system, in particular, the whole ascorbate-glutathione cycle. The less oxidative damage induced by Cd and the stress combination in the transgenics resulted not only from the GST and CAT1 transgene but also from the coordination of the whole ascorbate-glutathione cycle.

关键词： ascorbate-glutathione cycle catalase combined cadmium and heat stress glutathione-S-transferase transgenic rice

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Response of Gas Exchange and Water Use Efficiency to Light Intensity and Temperature in transgenic rice Expressing PEPC and PPDK Genes

引用

Agricultural Sciences in China 2009年第11期8卷 1312-1320页

作者： ZHANG Bian-jiang CHEN Quan-zhan HUA Chun ZHOU Feng ZHOU Quan-chen JIAO De-mao Nanjing Xiaozhuang University Nanjing 211171 P.R. China Jiangsu Academy of Agricultural Sciences Nanjing 210014 P.R. China

Aiming to controvert whether the photosynthetic capacity of transgenic rice expressing C4 genes is enhanced, with the C3-type untransformed rice （WT） and maize （a C4 plant） as controls, the activity of C4 photosynthetic enzymes, gas exchange parameters and water use efficiency （WUE） under different light intensities and temperatures, the stable carbon isotope ratio （8-3C） value and the metabolic index of active oxygen as well as plant yield parameters were determined in transgenic rice carrying the PEPC and PPDK genes （CK） in this study. The results showed that the light-saturated photosynthetic rate of CK was intermediate between that of WT and maize, with a slight bias towards that of maize. Under a high light intensity （1 200 μmol m^-2 s^-1） and high temperature （35℃）, CK still exhibited higher photosynthetic capacity, while the Gs decreased. The WUE of CK was only slightly increased, and was similar to that of WT. The δ13C value indicated that CK functioned as a C3 plant. In addition, the tolerance to photo-oxidation and grain yield of CK was enhanced by sprayed with NaHSO3. In conclusion, CK possesses higher photosynthetic productivity under the conditions of high photon flux density （PFD）, high temperature and spraying with NaHSO3 solution, thereby providing a new technical approach and physiological basis for constructing C4-like rice.

关键词： transgenic rice photosynthetic characteristics water use efficiency stomatal conductance

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Lethal and Sub-Lethal Effects of transgenic rice Containing cry1Ac and CpTI Genes on the Pink Stem Borer, Sesamia inferens(Walker)

引用

Agricultural Sciences in China 2011年第3期10卷 384-393页

作者： HAN Lan-zhi HOU Mao-lin WU Kong-ming PENG Yu-fa WANG Feng State Key Laboratory of Plant Disease and Insect Pests/Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing 100193P. R. China Key Laboratory of Agricultural Genetics Engineering Fujian Academy of Agricultural Sciences Fuzhou 350003 P.R.China

Lethal and sub-lethal effects of transgenic rice containing cry1Ac and CpTI genes on the pink stem borer, Sesamia inferens, were studied to collect information for ecological risk assessment on insect-resistance of transgenic rice. In vitro insect-feeding bioassays were conducted to evaluate the lethal and sub-lethal effects of transgenic rice lines （II YouKF6 and KF6） containing cry1Ac＋CpTI genes on S. inferens at four different growth stages, viz., seedling, tillering and elongation, booting, and milk and maturing. transgenic rice at seedling stage showed significantly high lethal effect on S. inferens with the shortest lethal duration for 50 and 100% individuals and the highest corrected mortalities after feeding on transgenic lines at this stage for 3 and 6 d. Followed by tillering and elongation stage, 50 and 100% S. inferens were dead after feeding on transgenic lines at this stage for 4 and 10 d, respectively. Moreover, corrected mortalities for 6 d feeding on transgenic lines at this stage were significantly higher than that at booting, and milk and maturing stages. Lethal effect of KF6 on S. inferens decreased significantly at booting stage. Lethal duration for 50% S. inferens significantly extended and its corrected mortalities for 6 d feeding also declined remarkably. However, lethal effect of II YouKF6 on S. inferens did not decrease significantly at this stage. transgenic rice at booting, and milk and maturing stages did not show significant lethal effect to S. inferens and it showed the longest lethal duration for 50% individuals and the lowest corrected mortalities for 3 and 6 d feeding. A few larvae of S. inferens could survive, pupate and emerge on these two transgenic lines at booting, and milk and maturing stages. Sub-lethal effect of two transgenic lines on S. inferens also differed significantly between different developmental stages. Continuously feeding on transgenic rice lines at seedling, and tillering and elongation stages delayed the devel

关键词： transgenic rice Sesamia inferens lethal and sub-lethal effect

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Impact of insect-resistant transgenic rice on target insect pests and non-target arthropods in China

引用

Insect Science 2006年第6期13卷 409-420页

作者： MAO CHEN JIAN-ZHOU ZHAO GONG-YIN YE QIANG FU ANTHONY M. SHELTON Department of Entomology Cornell University-NYSAES Geneva New York USA State Key Laboratory of rice Biology Institute of Applied Entomology Zhejiang University State Key Laboratory of rice Biology China National Rice ResearchInstitute Chinese Academy of Agricultural Sciences Hangzhou Zhejiang China

Progress on the research and development of insect-resistant transgenic rice, especially expressing insecticidal proteins from Bacillus thuringiensis （Bt）, in China has been rapid in recent years. A number of insect-resistant transgenic rice lines/varieties have passed restricted and enlarged field testing, and several have been approved for productive testing since 2002 in China, although none was approved for commercial use until 2006. Extensive laboratory and field trials have been conducted for evaluation of the efficiency of transgenic rice on target lepidoteran pests and potential ecological risks on non-target arthropods. The efficacy of a number of transgenic rice lines currently tested in China was excellent for control of the major target insect pests, the rice stem borers （Chilo suppressalis, Scirpophaga incertulas, Sesamia inferens） and leaffolder （ Cnaphalocrocis medinalis）, and was better than most insecticides extensively used by millions of farmers at present in China. No significantly negative or unintended effects of transgenic rice on non-target arthropods were found compared with non-transgenic rice. In contrast, most of the current insecticides used for the control of rice stem borers and leaffolders proved harmful to natural enemies, and some insecticides may directly induce resurgence of rice planthoppers. Studies for developing a proactive insect resistance management of transgenic rice in the future are discussed to ensure the sustainable use of transgenic rice.

关键词： Bt toxin insect resistance non-target effect target insects transgenic rice

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：