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Field released transgenic papaya effect on soil microbial communities and enzyme activities

Journal of Environmental Sciences 2006年第4期18卷 734-740页

作者： WEI Xiang-dong ZOU Hui-ling CHU Lee-min LIAO Bin YE Chang-min LAN Chong-yu School of Life Sciences Sun Yat-sen University Guangzhou 510275 China Department of Biology The Chinese University ofHong Kong Shatin Hong Kong China

Soil properties, microbial communities and enzyme activities were studied in soil amended with replicase （RP）-transgenic or non-transgenic papaya under field conditions. Compared with non-transgenic papaya, significant differences （P〈0.05） were observed in total nitrogen in soils grown with transgenic papaya. There were also significant differences （P〈0.05） in the total number of colony forming units （CFUs） of bacteria, actinomycetes and fungi between soils amended with RP-transgenic plants and non-transgenic plants. Compared with non-transgenic papaya, the total CFUs of bacteria, actinomycetes and fungi in soil with transgenic papaya increased by 0.43-1.1, 0.21-0.80 and 0.46-0.73 times respectively. Significantly higher （P〈0.05） CFUs of bacteria, actinomycetes and fungi resistant to kanamycin （Km） were obtained in soils with RP-transgenic papaya than those with non-transgenic papaya in all concentrations of Km. Higher resistance quotients for Km＇（kanamycin resistant） bacteria, actinomycetes and fungi were found in soil planted with RP-transgenic papaya, and the resistance quotients for Km＇ bacteria, actinomycetes and fungi in soils with transgenic papaya increased 1.6-4.46, 0.63-2.5 and 0.75-2.30 times. RP-transgenic papaya and non-transgenic papaya produced significantly different enzyme activities in arylsulfatase （5.4-5.9x）, polyphenol oxidase （0.7-1.4x）, invertase （0.5-0.79x）, cellulase （0.23-0.35x） and phosphodiesterase （0.16-0.2x）. The former three soil enzymes appeared to be more sensitive to the transgenic papaya than the others, and could be useful parameters in assessing the effects of transgenic papaya. transgenic papaya could alter soil chemical properties, enzyme activities and microbial communities.

关键词： transgenic papaya soil microbes enzyme activity kanamycin resistance resistance quotient

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Analysis on virus resistance and fruit quality for T4 generation of transgenic papaya

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Frontiers in Biology 2007年第3期2卷 284-290页

作者： WEI Xiangdong LAN Congyu LU Zhijing YE Changming The Key Laboratory of Gene Engineering of Ministry of Education The State Key Laboratory of Biological ControlSun Yat-sen UniversityGuangzhou 510275China College of Resources and Environment Hunan Agriculture UniversityChangsha 410128China

Molecular biological characterization,fruit char-acters,and nutrients were analyzed for T4 generation of transgenic *** transgenic papaya plants with the mutated replicase(RP)gene from papaya ringspot virus(PRSV)showed high resistance or immunity against PRSV in the *** RP transgene can be steadily inherited to,and expressed at RNA level,the *** growth char-acteristics of transgenic papaya were much better than non-transgenic papaya in the *** non-transgenic papaya seedlings began to show typical symptoms caused by PRSV after being inoculated with *** died quickly and never grew to produce *** adult trees developed yellow leaves and produced smaller fruits and were doomed to a slow death after some time,while most of transgenic papaya plants(about 91.8%)did not show any symptoms caused by PRSV,and produced more,bigger,and high quality *** with non-transgenic plants,the fresh fruit length of T4 gen-eration of transgenic papaya increased 2.6%-5%,and the diameter decreased 0.6%-1.5%.The flesh thickness of fresh fruit increased 12%-15%,which made it fitter for *** the fresh fruit quality changed,there was no sig-nificant difference between transgenic and non-transgenic *** quality characteristics of dry fruit including the contents of water,lipid,N,protein,reduced sugar,vitamin A,vitamin C,and carotene in the T4 generation of transgenic papaya were all the same as their non-transgenic *** means that transgenic plants and non-transgenic plants are substantially equivalent,and the transgene has no effect on dry fruit *** this study,we found that vitamin A and vitamin C in red-fleshed papaya were 1.4-1.8 and 1.78-2.07 times more than the yellow-fleshed ones,respectively,while N and protein were only 84.2%-92.1%and 82.1%-98.9%of the yellow-fleshed ones.

关键词： transgenic papaya molecular detection fruit quality

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Inheritance of papaya Ringspot Virus Coat Protein Transgene and Viral Resistance in the Progeny of transgenic papaya in Thailand

Inheritance of Papaya Ringspot Virus Coat Protein Transgene ...

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第二届植物分子育种国际会议

作者： S.Kertbundit N.Pungtanom A.Tanwanchai P.Ruangchan M.Juricek Department of Plant Science Faculty of ScienceMahidol UniversityBangkok10400Thailand Institute of Experimental Botany Academy of Sciences of the Czech RepublicNa Pernikarce 15160 00 Prague 6Czech Republic Institute of Molecular Biology and Genetics Mahidol UniversitySalaya CampusNakhon Pathom73170Thailand Institute of Molecular Biology and Genetics Mahidol UniversitySalaya CampusNakhon Pathom73170Thailand Institute of Molecular Biology and Genetics Mahidol UniversitySalaya CampusNakhon Pathom73170Thailand Institute of Experimental Botany Academy of Sciences of the Czech RepublicNa Pernikarce 15160 00 Prague 6Czech Republic

＜正＞papaya plantations all over the world are devastated by papaya ringspot virus type P （PRSV-P）. In order to introduce viral resistance to PRSV-P Thai isolate （PRSV-thP） in papaya ‘Khak Dum’ variety, the coa... 详细信息

关键词： papaya ringspot virus PRSV transgenic papaya Virus resistance papaya breeding

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