检索结果-南通市图书馆

tongxinluo Reverses the Hypoxia-suppressed Claudin-9 in Cardiac Microvascular Endothelial Cells

Chinese Medical Journal 2016年第4期129卷 442-447页

作者： Kun Liu Xiu-Juan Wang Yan-Ning Li Bin Li Jin-Sheng Qi Jing Zhang Yu Wang Department of Biochemistry Hebei Key Laboratory of Medical Biotechnology Hebei Medical University Shijiazhuang Hebei 050017 China Department of Molecular Biology Hebei Key Lab of Laboratory Animal Hebei Medical University Shijiazhuang Hebei 050017 China

Background： Claudin-5, claudin-9, and claudin-11 are expressed in endothelial cells to constitute tight junctions, and their deficiency may lead to hyperpermeability, which is the initiating process and pathological basis of cardiovascular *** tongxinluo （TXL） has satisfactory antianginal effects, whether and how it modulates claudin-5, claudin-9, and claudin-1 1 in hypoxia-stimulated human cardiac microvascular endothelial cells （HCMECs） have not been ***： In this study, HCMECs were stimulated with CoCl2 to mimic hypoxia and treated with ***, the messenger RNA （mRNA） expression of claudin-5, claudin-9, and claudin-l 1 was ***, the protein content and distribution of claudin-9, as well as cell morphological changes were evaluated after TXL ***, the distribution and content histone H3K9 acetylation （H3K9ac） in the claudin-9 gene promoter, which guarantees transcriptional activation, were examined to explore the underlying mechanism, by which TXL up-regulates claudin-9 in hypoxia-stimulated ***： We found that hypoxia-suppressed claudin-9 gene expression in HCMECs （F=7.244;P =0.011） and the hypoxia-suppressed claudin-9 could be reversed by TXL （F=61.911;P =0.000）, which was verified by its protein content changes （F=29.142;P =0.000）.Moreover, high-dose TXL promoted the cytomembrane localization of claudin-9 in hypoxia-stimulated HCMECs, with attenuation of cell ***, high-dose TXL elevated the hypoxia-inhibited H3K9ac in the claudin-9 gene promoter （F=37.766;P =0.000）, activating claudin-9 ***： The results manifested that TXL reversed the hypoxia-suppressed claudin-9 by elevating H3K9ac in its gene promoter, playing protective roles in HCMECs.

关键词： Cardiac Microvascular Endothelial Cells Claudin-9 H3K9 Acetylation Hypoxia tongxinluo

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

tongxinluo promotes mesenchymal stem cell tube formation in vitro

引用

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2011年第8期12卷 644-651页

作者： Xin-yang HU Wen-xia WANG Min-jia YU Xian-bao LIU Rong-rong WU Feng GAO Xin HUANG Jiang CAO Xiao-jie XIE Jian-an WANG Cardiovascular Key Lab of Zhejiang Province the Second Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310009 China Department of Cardiology the Second Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310009 China

Objective： To study whether tongxinluo （TXL） can induce angiogenesis in bone marrow mesenchymal stem cells （MSCs）, and to investigate the underlying mechanism. Methods： Bone marrow MSCs were obtained from male Sprague-Dawley rats. We established an angiogenesis model in vitro via matrigel experiment. MSCs were seeded on matrigel coated 24-well plates, and treated by TXL 50 and 100 mg/L. After 24 h, we observed the tube formations of MSCs in the matrigel. Cell migration ability was examined by wound scratch test and transwell assay. Expressions of vascular endothelial growth factor （VEGF）, fetal liver kinase-1 （FIk-1）, matrix metalloproteinase-2 （MMP-2）, MMP-9, and tissue inhibitor of metalloproteinase-2 （TIMP-2） were analyzed at the protein level by Western blot. Gelatin zymography assay was applied to investigating the MSC paracrine abilities of pro-MMP-2 and activated MMP-2. Results： TXL promoted MSC tube formation in matrigel. The ratio of TXL 100 mg/L treated-MSC tubular length was increased 3.04-fold compared to the control group （P〈0.05）. Scratch test and transwell assay showed that TXL could improve the cell migration ability of MSCs. Western blot experiments showed that TXL promoted MSC synthesis of MMP-2, but it had no influence on the expressions of MMP-9 and TIMP-2. This effect was confirmed by gelatin zymography assay, which showed that TXL increased MSC secretion of pro-MMP-2 and activated MMP-2. VEGF expression of TXL treated-MSCs was increased compared to the control group. The expression of FIk-1 was not different among the groups. Conclusions： This study demonstrates that TXL can promote the tube formation of MSCs, and the underlying mechanisms are associated with increased migration ability of MSCs and the up-regulation of MMP-2 and VEGF expressions.

关键词： Bone marrow mesenchymal stem cells tongxinluo Angiogenesis Migration Tube formation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

tongxinluo Inhibits Cyclooxygenase-2, Inducible Nitric Oxide Synthase, Hypoxia-inducible Factor-2α/Vascular Endothelial Growth Factor to Antagonize Injury in Hypoxia-stimulated Cardiac Microvascular Endothelial Cells

引用

Chinese Medical Journal 2015年第8期128卷 1114-1120页

作者： Yan-Ning Li Xiu-Juan Wang Bin Li Kun Liu Jin-Sheng Qi Bing-Hui Liu Ye Tian Department of Biochemistry Hebei Key Laboratory of Medical Biotechnology Hebei Medical University Shijiazhuang Hebei 050017 China Department of Molecular Biology Hebei Key Laboratory of Laboratory Animal Hebei Medical University Shijiazhuang Hebei 050017 China

Background： Endothelial dysflinction is considered as the initiating process and pathological basis of cardiovascnlar disease. Cyclooxygenase-2 （COX-2） and prostacyclin synthase （PGIS）, inducible nitric oxide synthase （iNOS） and endothelial NOS （eNOS） are key enzymes with opposing actions in inflammation and oxidative stress, which are believed to be the major driver of endothelial dysfunction. And in hypoxia （Hx）, Hx-inducible factor （HIF）-1α and HIF-2α are predominantly induced to activate vascular endothelial growth factor （VEGF）, restllting in abnormal proliferation. Whether and how tongxinluo （TXL） modulates COX-2, PGIS, iNOS, eNOS, HIF-1α, HIF-2α, and VEGF in Hx-stimulated human cardiac microvascular endothelial cells （HCM ECs） have not been clarified. Methods： HCMEC were treated with CoCl2 to mimic Hx and the mRNA expressions of COX-2, PGIS, iNOS, eNOS, HIF-1α, HIF-2α. and VEGF were first confirmed, and then their mRNA expression and protein content as well as the cell pathological alterations were evaluated for TXL treatment with different concentrations, In addition, the effector molecular of inflammation prostaglandin E2 （PGE2） and the oxidative marker nitrotyrosine （NT） was adopted to reflect HCMEC ***. Results： Hx could induce time-dependent increase of COX-2, iNOS, HIF-2α, and VEGF in HCMEC. Based on the Hx-induced increase, TXL could mainly decrease COX-2, iNOS, HIF-2α, and VEGF in a concentration-dependent manner, with limited effect on the increase of PGIS and eNOS. Their protein contents verified the mRNA expression changes, which was consistent with the cell morphological alterations. Furthermore, high dose TXL could inhibit the Hx-induced increase of PG E, and NT contents, attenuating the inflammatory and oxidative injury. Conclusions： TXL could inhibit inflammation-related COX-2, oxidative stress-related iNOS, and H IF-2α/VEGF to antagonize Hx-induced HCMEC injury.

关键词： Cyclooxygenase-2 Hypoxia-inducible Factor-2α Hypoxia Inducible Nitric Oxide Synthase tongxinluo Vascular Endothelial Growth Factor

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effects of Chinese medicine tongxinluo on hyperglycemia and beta-cell damage in streptozotocin-induced diabetic rats

引用

Chinese Medical Journal 2012年第20期125卷 3675-3680页

作者： WANG Huai-qing ZOU Jun-jie ZHOU Xiang-hai JI Li-nong LIU Zhi-min Department of Endocrinology and Metabolism Peking University People's HospitalPeking University Diabetes CenterBeijing 100044China Department of'Endocrinology and Metabolism First People's Hospital of YichangChina Three Gorges UniversityYichangHubei 443000China Department of Endocrinology'and Metabolism Changzheng HospitalSecondary Military Medical UniversityShanghai 200003China

Background Oxidative stress has been implicated in the onset and progression of diabetes. tongxinluo is a traditional Chinese medicine with potent antioxidant properties. The aim of this study was to test the hypothesis that pretreatment with tongxinluo has similar effects as melatonin on preventing hyperglycemia and beta-cell damage in a rat model of streptozotocin （STZ）-induced diabetes. Methods Forty male Sprague Dawley rats were randomly assigned to four groups （n=10 each）： normal control （NC） group; STZ group （70 mg/kg, i.p.）; tongxinluo （1.0 g-kg-^-d-1） pretreated （TXL＋STZ） group and melatonin （200 iJg-kg-~.d-1） pretreated （MLT＋STZ） group. tongxinluo and melatonin were administered by gavage beginning 8 days before STZ injection and continuing until the end of the study （15 days after STZ administration）. Blood glucose levels and body weights, malondialdehyde （MDA）, and reduced glutathione （GSH） levels were measured, and immunofluorescence studies were performed in all of the groups. Results Pretreatment with tongxinluo, as with melatonin, attenuated severe hyperglycemia and weight loss induced by STZ.. In pancreatic homogenates, MDA levels were significantly lower and GSH levels were significantly higher in tongxinluo pretreated group and in melatonin pretreated group than those in STZ group. Values of insulin staining were significantly improved in tongxinluo pretreated group and in melatonin pretreated group as compared with those in STZ group. Conclusions tongxinluo, as melatonin, prevented hyperglycemia and beta-cell destruction induced by STZ in rats through reducing oxidative stress in pancreatic tissues. tongxinluo may provide an alternative therapy for the prevention and treatment of diabetes.

关键词： tongxinluo streptozotocin diabetes," oxidative stress," antioxidants beta-cell function

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Observation of changes in the number of myocardial capillaries in rabbits after treatment of acute myocardial infarction by tongxinluo superfine powder

引用

Journal of Traditional Chinese Medicine 2018年第3期38卷 406-411页

作者： Wu Haotian Zhang Fengshun Liu Jinyi Li Xuezhao Guo Fei Wu Yiling School of Basic Medicine Nanjing University of Traditional Chinese Medicine Nanjing 210029 China Pharmaceutical Research Institute of Yiling Shijia-zhuang 050035 China Key Laboratory of State of Traditional Chinese Medicine (Cardo-Cerebral Vessel Collateral Disease) the Traditional Research Room of Traditional Chinese Medicine Shijiazhuang 050000 China Key Disciplines of State Administration of Traditional Chinese Medicine for Collateral Disease Shiiiazhuang 050000 China Hebei University Health Science Center pathology Laboratory Baoding 071000 China

OBJECTIVE: To investigate the effects of tongxinluo superfine powder on cardiac function, infarct size and the number of myocardial capillaries in a rabbit model of acute myocardial ***: A total of 32 New Zealand white rabbits were randomly divided into four groups: sham operation group, model group, treatment group,and pre-treatment, the experiment of pre-treatment group was performed 6 weeks early than the treat) group,The four groups use a unified modeling technique. An acute myocardial infarction model was established through external application of70% ferric chloride on the coronary artery. After 7 d,electrocardiogram, ultrasonography of cardiac function, micro-computed tomography, pathology and other data were ***: In the treatment and pre-treatment groups, ejection fraction, left ventricular short axis shortening rate, left ventricular end-systolic diameter and cardiac output significantly improved, the number of capillaries significantly increased, and infarct size significantly decreased. In addition, the results suggest that the value of intra-ventricular pressure and the situation of electrocardiogram also changed to different degrees with the increasing of treatment of ***: tongxinluo superfine powder can protect the myocardium, improve the blood supply of the myocardium and reduce the degree of myocardial injury, during acute stage of myocardial infarction.

关键词： Myocardial infarction Myocardium Mi-crocirculation X-Ray Microtomography Ferric chlo-ride tongxinluo superfine powder

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

No-reflow protection and long-term efficacy for acute myocardial infarction with tongxinluo： a randomized double-blind placebo- controlled multicenter clinical trial （ENLEAT Trial）

引用

Chinese Medical Journal 2010年第20期123卷 2858-2864页

作者： ZHANG Hai-tao JIA Zhen-hua ZHANG Jian YE Zan-kai YANG Wei-xian TIAN Yue-qin JIA Xuan LI Wei WU Yi-ling YANG Yue-jin Department of Cardiology Fuwai Hospital and Cardiovascular InstitutePeking Union Medical College and Chinese Academy of Medical SciencesBeijing 100037China The Integration of Traditional and Western Medical Research Academy of Hebei Province ShijiazhuangHebei 050035China Department of Echocardiography Fuwai Hospital and Cardiovascular InstitutePeking Union Medical College and Chinese Academy of Medical SciencesBeijing 100037China Department of Nuclear Medicine Fuwai Hospital and Cardiovascular InstitutePeking Union Medical College and Chinese Academy of Medical SciencesBeijing 100037China Department of Biostatistics National Center for Cardiovascular DiseasesBeijing 100037China

Background No-reflow after emergency percutaneous coronary intervention （PCI） for acute ST segment elevation myocardial infarction （STEMI） is related to the severe prognosis. The aim of this study was to evaluate the efficacy of tongxinluo, a traditional Chinese medicine, on no-reflow and the infarction area after emergency PCI for *** A total of 219 patients （female 31, 14%） undergoing emergency PCI for STEMI from nine clinical centers were consecutively enrolled in this randomized, double-blind, placebo-controlled, multicenter clinical trial from January 2007 to May 2009. All patients were randomly divided into tongxinluo group （n=108） and control group （n=111）, given tongxinluo or placebo in loading dose 2.08 g respectively before emergency PCI with asprin 300 mg and clopidogrel 300 mg together, then 1.04 g three times daily for six months after PCI. The ST segment elevation was recorded by electrocardiogram at hospitalization and 1, 2, 6, 12, 24 hours after coronary balloon dilation to evaluate the myocardial no-flow; myocardial perfusion scores of 17 segments were evaluated on day 7 and day 180 after STEMI with static single-photon emission computed tomography （SPECT） to determine the infarct *** There was no statistical significance in sex, age, past history, chest pain, onset-to-reperfusion time, Killip classification, TIMI flow grade just before and after PCI, either in the medication treatment during the follow up such as statin, β-blocker, angiotensin converting enzyme inhibitor （ACEI） or angiotensin receptor blocker （ARB） between two groups. There was significant ST segment restoration in tongxinluo group compared to the control group at 6 hours （（-0.22±0.18） mV vs. （-0.18±0.16） mV, P=0.0394）, 12 hours （（-0.24 ± 0.18） mV vs. （-0.18±0.15） mV, P=0.0158） and 24 hours （（-0.27±0.16） mV vs. （-0.20±0.16） mV, P=0.0021） reperfusion; and the incidence of myocardial no-reflow was also reduced significantly at 24-hour reperfusion （34.3% vs. 54.1%,

关键词： acute myocardial infarction no-reflow percutaneous coronary intervention tongxinluo

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Improving cognitive impairment by tongxinluo via inhibiting expression of beta-secretase 1/beta-amyloid peptide in experimental vascular dementia

引用

Neural Regeneration Research 2008年第3期3卷 262-266页

作者： Jia Jia Wenbin Zhu Lihui Wang Yun Xu Department of Neurology Affiliated Drum Tower Hospital of Nanjing University Medical School Nanjing 210008 Jiangsu Province China The State Key Laboratory of Pharmaceutical Biotechnology Nanjing University Nanjing 210008 Jiangsu Province China

BACKGROUND： tongxinluo has been clinically proven to be effective in improving memory and cognitive function in patients with post-stroke vascular dementia. Is the mechanism related to the deposition of beta-amyloid peptide （A β ） in hippocampus？ OBJECTIVE： To observe the effect of tongxinluo on cognitive impairment in a mouse model with vascular dementia and the changes of A β deposition and β -secretase 1 （BACE1） expression. DESIGN： Randomized controlled study. SETTING： State Key Laboratory of Pharmaceutical Biotechnology of Nanjing University and Affiliated Drum Tower Hospital of Nanjing University Medical School. MATERIALS： The experiment was carried out in the State Key Laboratory of Pharmaceutical Biotechnology of Nanjing University and Affiliated Drum Tower Hospital of Nanjing University Medical School from March 2006 to January 2007. A total of 36 healthy Kunming mice, 18 of each gender, were chosen. The study was conducted in accordance with the National Regulations of Experimental Animal Administration, and all animal experiments were approved by the Committee of Experimental Animal Administration of Nanjing University. tongxinluo was provided by Shijiazhuang Yiling Pharmaceutical Co., Ltd. METHODS： All mice were randomly divided into 6 groups, including naive control （n=6）, sham-operated control （n=6） and experimental groups treated with different doses of tongxinluo （0.2, 0.4, and 0.6 g/kg/d; n=6 for each group） or vehicle （n=6）. Five groups were subjected to bilateral common carotid arteries （2-VO） occlusion to produce a vascular dementia model （no occlusion was performed in sham-operated group）. The mice in the tongxinluo treatment groups were intragastricly administered daily with a tongxinluo suspension （40 g/L in distilled water） at doses of 0.2, 0.4 or 0.6 g/kg/d from day 1 to day 30 post-surgery. The animals in vehicle, sham-operated and naive groups were administered an equal volume of distilled water. MAIN OUTCOME MEASURES： ①Escape latency time

关键词： vascular dementia tongxinluo β -amyloid protein β -secretase 1

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effects of tongxinluo-facilitated cellular cardiomyoplasty with autologous bone marrow-mesenchymal stem cells on postinfarct swine hearts

引用

Chinese Medical Journal 2007年第16期120卷 1416-1425页

作者： QIAN Hai-yan YANG Yue-jin HUANG Ji GAO Run-lin DOU Ke-fei YANG Guo-sheng LI Jian-jun SHEN Rui HE Zuo-xiang LU Min-jie ZHAO Shi-hua Department of Cardiology Fuwai Hospital and Cardiovascular Institute Peking Union Medical-College and Chinese Academy of Medical Sciences Beijing 100037 China Department of Nuclear Medicine Fuwai Hospital and Cardiovascular Institute Peking Union Medical-College and Chinese Academy of Medical Sciences Beijing 100037 China Department of Radiology Fuwai Hospital and Cardiovascular Institute Peking Union Medical-College and Chinese Academy of Medical Sciences Beijing 100037 China Department of Cardiology Renmin Hospital of Wuhan University Wuhan 430063 China

Background Treatment of ischemic heart disease remains an important challenge, though there have been enormous progresses in cardiovascular therapeutics. This study was conducted to evaluate whether tongxinluo （TXL） treatment around the transplantation of mesenchymal stem cells （MSCs） can improve survival and subsequent activities of implanted cells in swine hearts with acute myocardial infarction （AMI） and reperfusion. Methods Twenty-eight Chinese mini-pigs were divided into four groups including a control group （n=7）; group 2, administration of low-close TXL alone from the 3rd day prior to AMI to the 4th day post transplantation （n=-7）; group 3, MSCs alone （n=-7） and group 4, TXL ＋ MSCs （n=7）. AMI models were made by occlusion of the left anterior descending coronary artery for 90 minutes. Autologous bone marrow-MSCs （3×10^7 cells/animal） were then injected into the post-infarct myocardium immediately after AMI and reperfusion. The survival and differentiation of implanted cells in vivo were detected by immunofluorescent analysis. The data of cardiac function were obtained at baseline （1 week after transplantation） and endpoint （6 weeks after transplantation） by single photon emission computed tomography （SPECT） and magnetic resonance imaging （MRI）. Apoptosis was detected by TUNEL assay and the oxidative stress level was investigated in the post-infarct myocardium at endpoint. Results At endpoint, there was less fibrosis and inflammatory cell infiltration with more surviving myocardium in group 4 than in the control group. In group 4 the survival and differentiation of implanted MSCs were significantly improved more than that seen in group 3 alone （P〈0.0001）; the capillary density was also significantly greater than in the control group, group 2 or 3 both in the infarcted zone （P〈0.0001） and the peri-infarct zone （P〈0.0001）. MRI showed that parameters at baseline were not significantly different between the 4 groups. At endpoint, regional wall thickening and t

关键词： mesenchymal stem cells acute myocardial infarction transplantation tongxinluo

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Protective Effects and Potential Mechanism of tongxinluo on Mice with Thromboangiitis Obliterans Induced by Sodium Laurate

引用

Chinese Journal of Integrative Medicine 2023年第7期29卷 608-616页

作者： GU Jiao-jiao WEI Ya-ru MA Ku WANG Xiao-qi GAO Huai-lin Graduate School Hebei University of Chinese MedicineShijiazhuang050090China Department of Endocrinology Hebei Yiling HospitalShijiazhuang050091China

Objective To investigate the effects of tongxinluo(TXL)on thromboangiitis obliterans(TAO)and the underlying *** Ninety male C57/BL6J mice were randomly divided into 6 groups according to a random number table:the sham group,TAO model group,Compound Danshen Tablet(CDT)group,and the high-,medium-,and low-dose TXL *** mice except the sham group were injected with sodium laurate(0.1 mL,5 mg/mL)in the femoral artery to establish TAO mouse *** modeling,mice in the sham and TAO model groups were intragastrically administered 0.5%(w/v)sodium carboxymethylcellulose,mice in the CDT group were intragastrically administered 0.52 g/kg CDT,and mice in the TXL-H,TXL-M,and TXL-L groups were intragastrically administered 1.5,0.75,and 0.38 g/kg TXL,*** 4 weeks of gavage,the recovery of blood flow in the lower limbs of mice was detected by Laser Doppler *** pathological changes and thrombosis of the femoral artery were observed by morphological *** expressions of tumor necrosis factorα(TNF-α)and inducible nitric oxide synthase(iNOS)in the femoral artery wall were detected by HE *** of thromboxane B2(TXB2),6-keto-prostaglandin F1α(6-keto-PGF1α),endothelin-1(ET-1),interleukin(IL)-1βand IL-6 were measured using enzyme-linked immunosorbent assay(ELISA).Levels of activated partial thromboplastin time(APTT),prothrombin time(PT),thrombin time(TT)and fibrinogen(FIB)were detected by a fully automated biochemical *** TXL promoted the restoration of blood flow in the lower limbs,reduced the area of thrombosis in the femoral artery,and alleviated the pathological changes in the femoral artery ***,the levels of TXB2,ET-1,IL-6,IL-1β,TNF-αand iNOS were significantly lower in the TXL groups compared with the model group(P<0.05 or P<0.01),while the level of 6-keto-PGF1αwas significantly higher(P<0.01).In addition,APTT,PT,and TT were significantly prolonged in TXL groups compared with the model group(P<0

关键词： tongxinluo thromboangitis obliterans thrombosis inflammation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Early Intervention of tongxinluo(通心络) on Right Ventricular Function Assessed by Echocardiography in Rats with Pulmonary Arterial Hypertension Induced by Monocrotaline

引用

Chinese Journal of Integrative Medicine 2020年第12期26卷 913-920页

作者： GAO Lu LI Shao-dan ZHANG Yin LIU Yi YANG Ming-hui Department of Nanlou Traditional Chinese Medicine and Acupuncture Chinese People’s Liberation Army General HospitalBeijing(100853)China Department of Traditional Chinese Medicine Chinese People’s Liberation Army General HospitalBeijing(100853)China

Objective:To investigate the effect of early intervention of tongxinluo(通心络,TXL)on right ventricular function(RVF)of rats with pulmonary arterial hypertension(PAH)induced by monocrotaline(MCT).Methods:A total of 30 adult male Sprague-Dawley rats were assigned to 5 groups with complete random experiment design:Sham group(Sham),MCT group,TXL group,sildenafil(SIL)group and combination group(TXL+SIL),6 rats in each *** were injected with 50 mg/kg MCT solution for inducing PAH model except for those in the sham *** the day of modeling,rats of TXL,SIL and TXL+SIL groups were given TXL(1.2 g/kg),SIL(10 mg/kg)and combination solution(TXL:1.2 g/kg,SIL:10 mg/kg)respectively,and rats in Sham and MCT groups were given normal saline(5 m L/kg).The samples were collected and tested after 21 consecutive days of intragastric *** was used to measure the related indices of RVF,including pulmonary arterial flow spectrum,pulmonary artery diameter(PAD),right ventricular wall thickness(RVWT),right ventricular diameter(RVD),tricuspidannular plane systolic excursion(TAPSE),right atrium transverse diameter(RAT),and inferior vena cava diameter(IVCD).Elastic Verhoeff-Van Gieson staining was adopted to measure the percentage of wall thickness(WT%)of pulmonary ***-eosin staining was used to measure the cross-sectional area(CSA)of right ventricular ***:MCT-induced PAH rat model was successfully *** MCT group the wall of pulmonary arterioles exhibited a prominent-increase thickness,PAD,RVWT,RVD,RAT,IVCD,WT%,right ventricular hypertrophy index(RVHI)as well as CSA of RV cardiomyocyte significantly increased(all P<0.01),and TAPSE markedly decreased(P<0.01).At the same time,TXL prominently improved all of the above indices(all P<0.01).In comparison with SIL,TXL significantly reduced RVD(P<0.05)and decreased CAS of RV cardiomyocytes(P<0.01),but TAPSE in SIL group was much larger than in TXL group(P<0.01).Moreover,

关键词： tongxinluo early intervention pulmonary arterial hypertension right ventricular function echocardiography

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：