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tissue tropism and molecular characterization of a Japanese encephalitis virus strain isolated from pigs in southwest China

Tissue tropism and molecular characterization of a Japanese ...

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第七届中国畜牧科技论坛

作者： Yuan lei Wu rui Liu hanyang Wen xintian Huang xiaobo yiping Wen Ma xiaoping Yan qigui Huang yong Zhao qin Cao sanjie Research Center of Swine Disease College of Veterinary MedicineSichuan Agricultural University Laboratory of Zoonosis College of Veterinary MedicineSichuan Agricultural University

Since September 2012,an epidemic has been spreading among swine in a pig farm located in Sichuan province,southwest China,which has resulted in abortion,stillbirth,and fetal *** brains of stillborn pigs were collected and a previously unknown Japanese encephalitis virus(JEV),namely SCYA201201,was *** to the results of agarose gel diffusion precipitation,indirect immunofluorescence analysis,neutralization testing,reverse transcription PCR(RT-PCR) amplification,and physical and chemical testing,the virus was conformed to have the characteristics of *** virus titer in BHK-21 cells was 10 PFU/ml and the median lethal dose(LD50) to 3-week-old and 7-dayold mice was 1.99 loglO and 1.02 loglO PFU/LD50,*** results of tissue tropism for mice showed that the viral load in the brain was significantly higher than other organs,indicating that the isolate was strongly ***,the complete genome sequence of the isolate was determined and compared with other JEV *** analysis showed that the isolate belongs to genotype 1 and may be an imported *** isolate had88.4%nucleotide identity with the Chinese vaccine strain ***,there were 69 amino acid substitutions compared with the strain *** substitutions indicated that SCYA201201 was highly neurovirulent and infective,in accordance with the results of animal testing.

关键词： Japanese encephalitis virus isolate tissue tropism molecular characterization

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