检索结果-南通市图书馆

Chinese Optics Letters 2023年第12期21卷 54-60页

作者：徐健壹戴奕宣昂姚应涛刘少军俞婷婷朱䒟 Britton Chance Center for Biomedical Photonics MoE Key Laboratory for Biomedical PhotonicsWuhan National Laboratory for OptoelectronicsAdvanced Biomedical Imaging FacilityHuazhong University of Science and TechnologyWuhan 430074China

Ischemic stroke causes long-term disability and results in motor impairments.Such impairments are associated with structural changes in the neuromuscular junction(NMJ),including detailed morphology and three-dimensional(3D)distribution.However,previous studies only explored morphological changes of individual NMJs after stroke,which limits the understanding of their role in post-stroke motor impairment.Here,we examine 3D distributions and detailed morphology of NMJs in entire mouse muscles after unilateral and bilateral strokes induced by photothrombosis.The results show that 3D distributions and numbers of NMJs do not change after stroke,and severe unilateral stroke causes similar levels of NMJ fragmentation and area enlargement to bilateral stroke.This research provides structural data,deepening the understanding of neuromuscular pathophysiology after stroke.

关键词： tissue optical clearing optical imaging stroke neuromuscular junction

Effects of delayed repair of peripheral nerve injury on the spatial distribution of motor endplates in target muscle

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2022年第2期17卷 459-464页

作者： Dong-Dong Li Jin Deng Bo Jin Shuai Han Xin-Yi Gu Xue-Feng Zhou Xiao-Feng Yin Department of Trauma and Orthopedics Peking University People’s HospitalBeijingChina Department of Orthopedics PLA Strategic Support Force Medical CenterBeijingChina

Motor endplates(MEPs) are important sites of information exchange between motor neurons and skeletal muscle, and are distributed in an organized pattern of lamellae in the muscle. Delayed repair of peripheral nerve injury typically results in unsatisfactory functional recovery because of MEP degeneration. In this study, the mouse tibial nerve was transected and repaired with a biodegradable chitin conduit, immediately following or 1 or 3 months after the injury. Fluorescent α-bungarotoxin was injected to label MEPs. tissue optical clearing combined with light-sheet microscopy revealed that MEPs were distributed in an organized pattern of lamellae in skeletal muscle after delayed repair for 1 and 3 months. However, the total number of MEPs, the number of MEPs per lamellar cluster, and the maturation of single MEPs in gastrocnemius muscle gradually decreased with increasing denervation time. These findings suggest that delayed repair can restore the spatial distribution of MEPs, but it has an adverse effect on the homogeneity of MEPs in the lamellar clusters and the total number of MEPs in the target muscle. The study procedures were approved by the Animal Ethics Committee of the Peking University People's Hospital(approval No. 2019 PHC015) on April 8, 2019.

关键词： degeneration delayed repair lamellar cluster light-sheet microscopy motor endplates peripheral nerve injury three-dimensional distribution tissue optical clearing

MACS-W:A modified optical clearing agent for imaging 3D cell cultures

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Journal of Innovative optical Health Sciences 2024年第2期17卷 24-34页

作者： Xiang Zhong Chao Gao Hui Li Yuening He Peng Fei Zaozao Chen Zhongze Gu Dan Zhu Tingting Yu Britton Chance Center for Biomedical Photonics-MoE Key Laboratory for Biomedical Photonics Huazhong University of Science and Technology Wuhan Hubei 430074P.R.China Wuhan National Laboratory for Optoelectronics-Advanced Biomedical Imaging Facility Huazhong University of Science and Technology Wuhan Hubei 430074P.R.China School of optical and Electronic Infrmation Huazhong University of Science and Technology Wuhan Hubei 430074P.R.China State Key Laboratory of Bioelectronics School of Biological Science and Medical Engineering Southeast University NanjingJiangsu 210096P.R.China Institute of Biomaterials and Medical Devices Southeast University Suzhou Jiangsu 215163P.R.China

Three-dimensional(3D)cell cultures have contributed to a variety of biological research fields by filling the gap between monolayers and animal models.The modern optical sectioning microscopic methods make it possible to probe the complexity of 3D cell cultures but are limited by the inherent opaqueness.While tissue optical clearing methods have emerged as powerful tools for investigating whole-mount tissues in 3D,they often have limitations,such as being too harsh for fragile 3D cell cultures,requiring complex handling protocols,or inducing tissue deformation with shrinkage or expansion.To address this issue,we proposed a modified optical clearing method for 3D cell cultures,called MACS-W,which is simple,highly efficient,and morphology-preserving.In our evaluation of MACS-W,we found that it exhibits excellent clearing capability in just 10 min,with minimal deformation,and helps drug evaluation on tumor spheroids.In summary,MACS-W is a fast,minimally-deformative and fluorescence compatible clearing method that has the potential to be widely used in the studies of 3D cell cultures.

关键词： tissue optical clearing 3D cell cultures imaging

Ex-vivo confocal Raman microspectroscopy of porcine skin with 633/785-NM laser excitation and optical clearing with glycerol/water/DMSO solution

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Innovative optical Health Sciences 2021年第5期4卷 33-45页

作者： Ali Jaafar Malik H.Mahmood Roman Holomb LászlóHimics Tamás Váczi Anton Y.Sdobnov Valery V.Tuchin Miklós Veres Institute for Solid State Physics and Optics Wigner Research Centre for Physics P.O.Box 49 H-1121 BudapestHungary Institute of Physics University of Szeged Dom ter 9H-6720 SzegedHungary Ministry of Higher Education and Scientific Research Baghdad 10065 Iraq Physiology Department College of Medicine University of MisanAl-AmarahMisan 62001Iraq Uzhhorod National University Uzhhorod 88015 TranscarpathiaUkraine Science Medical Center Saratov State University 83 Astrakhanskaya Str.Saratov 410012Russia Optoelectronics and Measurement Techniques Laboratory University of Oulu 90570 OuluFinland Laboratory of Laser Diagnostics of Technical and Living Systems Institute of Precision Mechanics and Control of the Russian Academy of Sciences 24 Rabochaya Saratov 410028Russia Interdisciplinary Laboratory of Biophotonics National Research Tomsk State University 36 Lenin Avenue Tomsk 634050Russia

Confocal Raman microspectroscopy(CRM)with 633-and 785-nm excitation wavelengths combined with optical clearing(OC)technique was used for ex-vivo study of porcine skin in the Raman fingerprint region.The optical clearing has been performed on the skin samples by applying a mixture of glycerol and distilled water and a mixture of glycerol,distilled water and chemical penetration enhancer dimethyl sulfoxide(DMSO)during 30 min and 60 min of treat-ment.It was shown that the combined use of the optical clearing technique and CRM at 633 nm allowed one to preserve the high probing depth,signal-to-noise ratio and spectral resolution simultaneously.Comparing the effect of different optical clearing agents on porcine skin showed that an optical clearing agent containing chemical penetration enhancer provides higher optical clearing efficiency.Also,an increase in treatment time allows to improve the optical clearing efficiency of both optical clearing agents.As a result of optical clearing,the detection of the amide-Ⅲ spectral region indicating well-distinguishable structural differences between the type-Ⅰ and type-Ⅳ collagens has been improved.

关键词： CRM skin collagen typesⅠandⅣ amideⅢ tissue optical clearing glycerol DMSO

Varying of up-conversion nanoparticles luminescence from the muscle tissue depth during the compression

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Innovative optical Health Sciences 2021年第5期4卷 85-92页

作者： Marina Kozintseva Vyacheslav Kochubey Julia Konyukhova Valery Tuchin Saratov State University 83Astrakhanskaya st.Saratov 410012Russia Tomsk State University 36Lenin Avenue Tomsk 634050Russia Tsinghua-Berkeley Shenzhen Institute Building C2&C3 Zhiyuan No.1001 Xueyuan Avenue Nanshan District.ShenzhenP.R.China

The current work is focused on the study of optical clearing of skeletal muscles under local compression.The experiments were performed on in vitro bovine skeletal muscle.The time dependence of optical clearing was studied by monitoring the luminescence intensity of NaYF_(4)∶Er,Yb upconverting particles located under tissue layers.This study shows the possibility to use upconverting nanoparticles(UCNPs)both for studying the dynamics of the optical clearing of biological tissue under compression and to detect moments of cell wall damage under excessive pressure.The advantage of using UCNPs is the presence of several bands in their luminescence spectra,located both at close wavelengths and far apart.

关键词： Upconverting particle biological tissue skeletal muscle tissue tissue optical clearing luminescence imaging technique mechanical compression

ALTERATIONS IN AUTOFLUORESCENCE SIGNAL FROM RAT SKIN EX VIVO UNDER optical IMMERSION clearing

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Innovative optical Health Sciences 2010年第3期3卷 147-152页

作者： E.V.MIGACHEVA A.B.PRAVDIN V.V.TUCHIN Saratov State University 83 Astrakhanskaya str.410012 SaratovRussia

For the first time,the changes in autofluorescence spectra of ex vivo rat skin have been experimentally investigated using the combination of fluorescence spectroscopy and optical immersion clearing.The glucose,glycerol and propylene glycol solutions were used as clearing agents.The optical clearing was performed from the dermal side of skin imitating the in vivo injection of clearing agent under the dermal layers.In this contribution,the common properties of autofluorescence variation during optical immersion clearing were determined.The tendency of autofluorescence signal to decrease with reduction of scattering in tissue was noticed and discussed in detail.However,the differences in the shape of spectral curves under application of different clearing agents showed that optical clearing affects the autofluorescence properties of tissue differently depending on the type of clearing liquid.The results obtained are useful for the understanding of tissue optical clearing mechanisms and for improving techniques such as fluorescence spectroscopy.

关键词： Autofluorescence spectra fluorescence spectroscopy rat skin tissue optical clearing clearing agents

optical MEA SUREMENTS OF RAT MUSCLE SAMPLES UND ER TREATMENT WITH ETHYLENE GLYCOL AND GLUCOSE

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Innovative optical Health Sciences 2013年第2期6卷 36-50页

作者： LUÍS OLIVEIRA MARIAINÊS CARVALHO ELISABETE NOGUEIRA VALERYV.TUCHIN Physics Department-Polytechnic of Porto School of Engineering Rua Dr.Antònio Bernardino de Almeida4314200-072 PortoPortugal University of Porto Rua Dr.Roberto Frias 4200-465 Porto Portugal CIETI-Centre of Innovationin Engineering and Industrial Technology ISEPRua Dr.Antònio Bernardino de Almeida4314200-072 PortoPortugal DEEC/FEUP and INESC TEC University of Porto Rua Dr.Roberto Frias4200-465 PortoPortugal Research-Educational Institute of Optics and Biophotonics Saratov State University 83 Astrakhanskaya Str.Saratov 410012Russia Laboratory of Laser Diagnostics of Technical and Living Systems Institute of Precise Mechanics and Control RASSaratov 410028Russia Optoelectronics and Measurement Techniques Laboratory P.O.Box 4500 University of OuluFIN-90014OuluFinland

With the objective to study the variation of optical properties of rat muscle during optical clearing,we have performed a set of optical measurements from that kind of tissue.The.measurements performed were total tr ansmittance,ollimated transmit tance,specular reflec-tance and total reflectance.This set of measurements is suficient to determine diffuse reflectance and absorbance of the sample,also necessary to est imate the optical properties.All the per formed measurements and calculated quantities will be used later in inverse Monte Carlo(IMC)simu-lations to determine the evolution of the optical properties of muscle during treatments with ethylene glycol and glucose.The results obt ained with the measurements already provide some information about the optical c learing treatments applied to the muscle and translate the mechanisms of turning the tissue more transparent and sequence of regimes of optical clearing.

关键词： tissue optical clearing optical transmittance optical reflectance optical absorbance agent difusivity