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No DCX-positive neurogenesis in the cerebral cortex of the adult primate

Neural Regeneration Research 2020年第7期15卷 1290-1299页

作者： Ruo-Xu Liu Jie Ma Bin Wang Tian Tian Ning Guo Shao-Jun Liu Institute of Military Cognition and Brain Sciences BeijingChina Department of Pharmacy Medical CollegeHuanghe S&T UniversityZhengzhouHenan ProvinceChina

Whether endogenous neurogenesis occurs in the adult cortex remains *** increasing number of reports suggest that doublecortin(DCX)-positive neurogenesis persists in the adult primate cortex,attracting enormous attention *** this study,different DCX antibodies were used together with NeuN antibodies in immunohistochemistry and western blot assays using adjacent cortical sections from adult *** adsorption,antigen binding,primary antibody omission and antibody-free experiments were used to assess specificity of the *** found either strong fluorescent signals,medium-weak intensity signals in some cells,weak signals in a few perikarya or near complete lack of labeling in adjacent cortical sections incubated with the various DCX *** putative DCX-positive cells in the cortex were also positive for NeuN,a specific marker of mature ***,further experiments showed that most of these signals were either the result of antibody cross reactivity,the non-specificity of secondary antibodies or tissue *** confirmed DCX-positive cells were detected in the adult macaque cortex by *** findings show that DCX-positive neurogenesis does not occur in the cerebral cortex of adult primates,and that false-positive signals(artefacts)are caused by antibody cross reactivity and *** experimental protocols were approved by the Institutional Animal Care and Use Committee of the Institute of Neuroscience,Beijing,China(approval ***-AMMS-2014-501).

关键词： adult neurogenesis antigen neutralization autofluorescence cross reaction cross reactivity doublecortin neocortex NeuN non-specificity primates tissue adsorption

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