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thymic stromal lymphopoietin expression is increased in nasal epithelial cells of patients with mugwort pollen sensitive-seasonal allergic rhinitis

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Chinese Medical Journal 2009年第19期122卷 2303-2307页

作者： ZHU Dong-dong ZHU Xue-wei JIANG Xiao-dan DONG Zhen Department of Otorhinolarygology Head & Neck Surgery China-Japan Union Hospital Jilin University Changchun Jilin 130033 China

Background Excessive expression of thymic stromal lymphopoietin （TSLP） has been demonstrated in asthmatic airway epithelia and in nasal epithelia from animal models of allergic rhinitis （AR）, but the evidence of expression of TSLP in nasal epithelial cells （NECs） of patients with AR is lacking. We aimed to investigate the expression of TSLP in NECs of patients with mugwort sensitive-seasonal AR and determine whether it is associated with severity of symptoms and the number of infiltrated eosinophils in nasal mucosa. Methods NECs specimens were obtained by scraping with plastic curettes from the nasal inferior turbinates of patients with mugwort pollen sensitive-seasonal AR （n=22） and nonallergic controls （n=11） during last peak mugwort pollen season. The severity of nasal symptom was assessed using a Visual Analog Scale （VAS）. In addition, serum mugwort pollen IgE levels were tested from each patient. In situ hybridization （ISH） was performed to test the messenger RNA （mRNA） of TSLP in the NECs. Furthermore, immunohistochemical staining （IHC） was scored to evaluate the expression of TSLP and eosinophil cell count was made by May-Grunwald/Giemsa staining. The correlation between expression of TSLP and all other parameters was analyzed in this study. Results The mRNA level of TSLP was significantly increased in NECs of patients with AR compared with the nonallergic control group （P 〈0.05）. In addition, IHC results showed that expression of TSLP in NECs from patients with AR was up-regulated which was correlated with VAS score （r=0.598; P 〈0.05） and nasal eosinophils count （r=0.702; P 〈0.05）, but it was unrelated with mugwort pollen specific IgE level. Conclusions These preliminary findings indicate a potential relationship between TSLP expression, severity of symptoms and nasal eosinophils count in pathogenesis of AR, but TSLP expression did not correlate with mugwort pollen specific IgE level. The elevated expression of TSLP might play a critical role in local atopic

关键词： rhinitis, allergic expression thymic stromal lymphopoietin mugwort pollen, pathogenesis

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Interactions of thymic stromal lymphopoietin with interleukin-4 in adaptive immunity during Aspergillus fumigatus keratitis

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International Journal of Ophthalmology(English edition) 2021年第10期14卷 1473-1483页

作者： Chen Chen Chen-Yang Dai Fang Han Jia-Yin Wu Lin Sun Xin-Yi Wu Department of Ophthalmology Clinical Medical College of Shandong UniversityJinan 250012Shandong ProvinceChina Department of Ophthalmology Qilu Hospital of Shandong UniversityJinan 250012Shandong ProvinceChina Key Laboratory of Cardiovascular Remodeling and Function Research Shandong UniversityJinan 250012Shandong ProvinceChina

AIM:To investigate the potential interactions of thymic stromal lymphopoietin(TSLP)with interleukin-4(IL-4)in adaptive immunity during fungal keratitis(FK).METHODS:An FK mouse model was induced with Aspergillus fumigatus(AF)hyphal *** were divided into several groups:untreated,phosphate buffer saline(PBS),infected with AF,and pretreated with a scrambled siRNA,a TSLP-specific siRNA(TSLP siRNA),murine recombinant TSLP(rTSLP),immunoglobulin G(IgG),murine recombinant IFN(rIFN-γ),murine recombinant IL-4(rI L-4),rIL-13,murine recombinant IL-17A(rIL-17A),and murine recombinant IL-17F(rIL-17F)*** realtime reverse transcription-polymerase chain reaction(qRTPCR)and enzyme-linked immunosorbent assay(ELISA)or Western blot were performed to determine mRNA and protein levels in the inflamed *** locations were observed by immunofluoresence staining after AF hyphal ***:Compared to those in the untreated group,TSLP and T helper type 1(Th1)cytokine levels in the AF group were upregulated at 24 h post infection(hpi),and those of T helper type 2(Th2)and T helper type 17(Th17)cytokines were increased at 5 d post infection(dpi).Th2 cytokine levels were decreased in the TSLP siRNA-pretreated group and increased in the rTSLP-pretreated group compared with the AF *** TSLP level was increased in the rIL-4-pretreated group,but there were no significant changes among the other *** staining showed cytokine locations after AF hyphal ***:TSLP induces a Th2 immune response and promots Th2 T cell differentiation in ***-4 promotes TSLP ***,TSLP with IL-4 regulates adaptive immunity in FK.

关键词： Aspergillus fumigatus keratitis thymic stromal lymphopoietin Th2 immune response interleukin-4

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Topical Tetracycline Improves MC903-induced Atopic Dermatitis in Mice through Inhibition of Inflammatory Cytokines and thymic stromal lymphopoietin Expression

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Chinese Medical Journal 2016年第12期129卷 1483-1490页

作者： Xiao-Jing Liu Zhang-Lei Mu Yan Zhao Jian-Zhong Zhang Department of Dermatology Peking University People's Hospital Beijing 100044 China

Background： Tetracycline （TET） has been found to have both antibiotic and anti-inflammatory properties. The anti-inflammatory effect of topical TET on atopic dermatitis （AD） has not been reported. The purpose of this study was to explore the potential role of topical TET and its anti-inflammatory effects in a mouse model of AD. Methods： The 2% TET was applied topically to ears of MC903-induced AD-like BALB/c mice once a day. AD-like symptoms and severity were evaluated by assessing skin scoring of dermatitis, ear thickness, and frequency of scratching. Serum lgE and thymic stromal lymphopoietin （TSLP） levels were measured by enzyme-linked immunosorbent assay. Western blot was used for analyzing the expressions of TSLP, protease-activated receptor 2 （PAR2）, and nuclear factor-kappa B （NF-~：B） in skin lesions. Real-time polymerase chain reaction was performed to assess the mRNA levels of TSLP and inflammatory cytokines including interleukin （IL）-4, IL-13, tumor necrosis factor （TNF）-α, and IL-1β in skin lesions. Results： Scoring of dermatitis （9.00 ± 0.63 vs. 6.67± 1.03, P = 0.001）, ear thickness （0.44± 0.02 mm vs. 0.40±0.03 mm, P = 0.018）, and serum IgE level （421.06 ± 212.13 pg/ml vs. 244.15±121.39 pg/ml, P 0.047） were all improved in the 2% TET treatment group compared with AD group. Topical TET significantly reduced the serum level of TSLP （119.04 ±38.92 pg/ml vs. 65.95± 54.61 μg/ml, P = 0.011） and both mRNA and protein expressions of TSLP in skin lesions compared with AD group （P = 0.003 and 0.011, respectively）, and NF-κB and PAR2 expression in skin lesions were also suppressed （P = 0.016 and 0.040, respectively）. Furthermore, expressions of inflammatory cytokines IL-4, IL- 13, and TNF-α in skin lesions were down-regulated in 2% TET group compared with AD group （P = 0.035, 0.008, and 0.044, respectively）. Conclusions： Topical TET exerted anti-inflammatory effects through suppression of TSLP and inflammatory cytokines in AD mouse model, suggesting TET as a potent

关键词： Atopic Dermatitis Tetracycline thymic stromal lymphopoietin

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Reduction of natural regulatory T cells in thymomas accompanying myasthenia gravis and its possible association with Foxp3 and thymic stromal lymphopoietin

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Journal of Medical Colleges of PLA(China) 2009年第1期24卷 50-55页

作者： Wang Zhongkui Wang Jinghua Deng Benqiang Ding Suju Wu Tao Department of Neurology Changhai Hospital Second Military Medical University Shanghai 200433 China

Objective: To investigate the expression of both thymic regulatory T cells (CD4+CD25+Foxp3+cells, Treg) and thymic stromal lymphopoietin (TSLP) in thymomas accompanying myasthenia gravis. Methods: We used immunohistochemistry and real-time reverse trancription polymerase chain reaction (real-time RT-PCR) techniques to determine Foxp3+ Treg counts and the expression levels of Foxp3 mRNA and TSLP mRNA in thymomas of 23 MG patients and thymuses of 4 healthy controls. Results: The CD4+ Foxp3+ nTreg (natural regulatory T cells) counts in thymomas were significantly lower than those in normal thymuses (P<0.01), and the expression levels of Foxp3 mRNA and TSLP mRNA were also lower in thymomas(P<0.01). Among the thymoma types, type B1 thymoma had the highest Foxp3+ nTreg count and standard values of Foxp3 mRNA and TSLP mRNA. There was a strong positive correlation between the mRNA transcriptional levels of Foxp3 and TSLP. Conclusion: The insufficient expression of Foxp3 in thymoma, which may be caused by decreased transcription of TSLP, may result in the reduction of Tregs and cause autoimmune disorders.

关键词： Myasthenia gravis Thymoma Regulatory T cell Foxp3 thymic stromal lymphopoietin

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Human TSLP-Educated DCs

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Cellular & Molecular Immunology 2008年第2期5卷 99-106页

作者： Jiongkun Wang Feiyue Xing Institute of Tissue Transplantation and Immunology Jinan UniversityGuangzhou 510632 China

thymic stromal lymphopoietin （TSLP）, an IL-7-related cytokine, is widely expressed by epithelial cells in many tissues with different biological effects. Human TSLP （hTSLP） has been shown to play an important role in promoting T cell homeostasis, developing nondeletional central tolerance, amplifying epithelium-induced class switching, inducing atopic diseases and maintaining intestinal noninflammatory environment. Among diverse cells responding to hTSLP, dendritic cells （DCs） are the most obviously characterized target cells. In this review, we attempt to outline an effect of the functional versatility of hTSLP-activated DCs （hTSLP-DCs） on T cells. Cellular ＆ Molecular Immunology.

关键词： thymic stromal lymphopoietin OX40 ligand dendritic cell T cell

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thymic stromal Lmphopoietin Pomotes Macrophage-derived Foam Cell Formation

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2014年第1期34卷 23-28页

作者：李大主王博远杨保杰何少林林静董江川吴纯胡骏 Department of Cardiology Union Hospital Tongji Medical College Huazhong University of Science and Technology

The effect of thymic stromal lymphopoietin（TSLP） on macrophage-derived foam cell formation and the underlying mechanism were studied. Macrophages isolated from C57BL/6 mice were co-cultured in vitro with different concentrations of TSLP or TSLPR-antibody in the presence of oxidized low density lipoprotein（ox-LDL）. The effects of TSLP on macrophage-derived foam cell formation were observed by using oil red O staining and intracellular lipid determination. The expression levels of foam cell scavenger receptors（CD36 and SRA） as well as ABCA1 and TSLPR were detected by using RT-PCR and Western blotting. As compared with the control group, TSLP treatment significantly promoted lipid accumulation in macrophages, significantly increased protein expression of CD36 and TSLPR in a dose-dependent manner, and significantly reduced the expression of ABCA1 protein in a dose-dependent manner. No significant differences were noted between the TSLPR-antibody group and the control group. TSLP may down-regulate the expression of cholesterol efflux receptor ABCA1 and up-regulate scavenger receptor expression via the TSLPR signaling pathway, thereby promoting macrophage-derived foam cell formation.

关键词： thymic stromal lymphopoietin atherosclerosis foam cells

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