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Celastrus orbiculatus Extract Inhibits Immune Inflammatory thrombotic state of B-Lymphoma

Chinese Journal of Integrative Medicine 2024年第11期30卷 1018-1026页

作者： ZHU Miao SHI Qing-qing NI Jun WU Wei SUN Xing SUN Mei XU Kai-lin LIU Yan-qing GU Jian GU Hao Department of Hematology Clinical Medical CollegeYangzhou UniversityYangzhouJiangsu Province 225001China Yangzhou Institute of Hematology YangzhouJiangsu Province 225001China Department of Hematology The Affiliated Hospital of Xuzhou Medical UniversityXuzhouJiangsu Province 221000China Department of Oncology The Affiliated Hospitalof Yangzhou UniversityYangzhou UniversityYangzhouJiangsu Province 225001China Department of Neurology The Affiliated Brain Hospital of Nanjing Medical UniversityNanjing 210000China

Objective:To investigate the inhibitory effect of Celastrus orbiculatus extracts(COE)on the proliferation of lymphoma cells and the immune regulation ability on inflammation and thrombophilia in ***:The 38B9 lymphoma cells were treated with COE(160μg/mL)and CTX(25μmol/L).The apoptosis rate and cell cycle of each group were detected by flow *** secretion of inflammatory factors,including interleukin(IL)-6,IL-10,and tumor necrosis factorα(TNF-α),in cell supernatant was detected by enzyme-linked immunosorbent assay(ELISA).In vivo,BALB/c mice were subcutaneously injected with 38B9 lymphoma cells to establish lymphoma ***(3 mg·kg^(-1)·d^(-1))and CTX(40 mg·kg^(-1)·d^(-1))were administered to the model mice,*** expression of plasma inflammatory factors(IL-6,IL-10 and TNF-α)and thrombus indexes,including D-dimer(D-D),von Willebrand factor(vWF)and tissue factor(TF),were detected by ELISA before tumor bearing(1 d),after tumor formation(14 d)and after intervention(21 d).PicoGreen dsDNA was used to detect the level of serum neutrophil extracellular traps(NETs).Flow cytometry was used to detect the expression of platelet activation marker calcium-dependent lectin-like receptor 2(CLEC-2).The tumor growth and survival of mice were ***:The 38B9 lymphoma cells were apoptotic after the intervention of COE and *** ratio of G_(2)-M phase cells decreased in COE intervented cells compared with the control cells(P<0.05),and S phase cells decreased in CTX intervented cells(P<0.05).Also,the secretion level of IL-6 was significantly reduced after COE or CTX intervention(P<0.05),and IL-10 was significantly increased(P<0.05).Furthermore,the tumor mass was reduced,and the median survival time was longer in COE and CTX intervented tumor-bearing mice than in non-intervented *** significantly lower levels of TNF-α,IL-6,NETs,TF,DD and CLEC-2,as well as higher IL-10 were observed in COE and CTX treatment mice in comparision with the control

关键词： Celastrus orbiculatus extract murine lymphoma cells tumor inflammation thrombotic state immune regulation proliferation inhibition lymphoma

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