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Effects of strain, body mass, and thread tip preparation on the establishment of focal cerebral ischemia mouse models

引用

Neural Regeneration Research 2008年第1期3卷 61-65页

作者： Xuemei Chen Yun Xu Department of Neurology Gulou Hospital Affiliated to Medical College Nanjing University Nanjing 210008 Jiangsu Province China

BACKGROUND： What are the successful factors of the establishment of the thread-blocking method for focal cerebral ischemia/reperfusion mouse models？ OBJECTIVE： To study the effects of strain, body mass, and thread tip preparation for the establishment of focal cerebral ischemia mouse models by using middle cerebral artery occlusion （MCAO）. DESIGN： Observational contrast animal study. SETTING： Gulou Hospital, Medical College of Nanjing University. MATERIALS：（1） The following experiment was performed at the Animal Experimental Center, Gulou Hospital Affiliated to Medical College of Nanjing University from December 2006 to April 2007. Sixty male white Kunming mice, whose body masses were 18-22 g （n =40）, 25-29 g （n =10） and 30-33 g （n =10）, as well as 10 male C57BL/6J mice, whose body mass was 18-22 g, were provided by the Animal Experimental Center, Gulou Hospital Affiliated to Medical College of Nanjing University. All mice were 10-12 weeks old. The project received confirmed consent from the local ethics committee. （2）Experimental materials： tripheryltetrazolium hydrochloride （TTC） and 0.1% poly-L-lysine were provided by Sigma Company, USA; citromint was provided by Shanghai Lingfeng Chemical Company Limited. methodS：（1） Strain comparison： Ten white Kunming mice （weighing 18-22 g） and ten C57BL/6J mice （weighing 18 - 22 g） were selected. （2） Comparison of body mass： Thirty white Kunming mice, whose body masses were 18-22 g （n =10）, 25-30 g （n =10）, and 30-35 g （n =10）, were divided into groups. （3） Comparison of thread tip preparation： White Kunming mice weighing 18-22 g were divided into a poly-L-lysine line group and general line group, with 10 mice in each group. Mice in these two groups, which were respectively treated with poly-L-lysine or nothing, underwent MCAO. （4） All experimental mice received MCAO. Three hours after ischemia, and 24 hours after reperfusion, neurological deficit scores were measured and a success rate of model establishment was calculate

关键词： mice ischemia/reperfusion animal model thread-blocking method

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