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THE　thermosensitivity　OF　HUMAN　GINGIVAL　SQUAMOUS　CARCINOMA　Ca9－22　CELLS　WITH　ONCOGENE　erbB－1／EGFR

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Chinese Journal of Cancer Research 1994年第4期6卷 259-265页

作者：张珊文 Beijing Institute for Cancer Research Beijing China

The heat sensitivity of Human gingival squamous carcinoma Ca9-22 cells with oncogene erbB-1/EGFR and Chinese hamster V79 cells of normal thermosensitivity as control was investigated.Colony forming ability of the treated cells was assayed in vitro. Heat-treatment period-survival and the concerned curves were drawn.The slopes of exponentially regressing parts of the survival curves were estimated in the TO values of cellular thermosensitivity and subjected to Arrhenius analysis-The 42-44℃ time-survival curves of Ca9-22 cells showed biphasic slopes which indicated the presence of thermotolerance induction during continuous heating even at 44℃ while for the V79 cells the biphasic slopes due to thermotolerance induction were shown in temperatures at and before 42℃. In comparison of TO values with V79 cells, those of Ca9-22 cells were longer (less thermosensitive) by about 2.1 fold at 43℃ and 1-2 fold at 44℃. Both V79 and Ca9-22 cells were sensitized by 44--42℃ step-down heating (SDH).42℃ beat treatment period-survival curves of 44℃ ( 5minute) preheated V79 cells; while Ca9-22 cells under the same treatment condition for not only 5 minutes but 20 minutes showed biphasic slope, which indicated the Presence of thermotolerance. The thermosensitization ratio (TSR) of Ca9-22 cells were smaller than V79 cells.Arrhenius curves a breaking points at 44℃ and 43℃ for Ca9-22 and V79 cells, respectively. The activation energies of V79 cells were 145kcallmole and 400kcal/mole above and below 43℃ (p thermosensitivity and variety in term of G￣1 phase as shown in fractionated Hydroxyurea treatment.

关键词： thermosensitivity Oncogene.

THERMOSENSITIVE POLYION COMPLEX MICELLES PREPARED BY SELF-ASSEMBLY OF TWO OPPOSITELY CHARGED DIBLOCK COPOLYMERS

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Chinese Journal of Polymer Science 2013年第2期31卷 318-324页

作者： Pan He Chang-wen Zhao Chun-sheng Xiao Zhao-hui Tang 陈学思 Key Laboratory of Polymer Ecomaterials Changchun Institute of Applied ChemistryChinese Academy of Science Graduate School of Chinese Academy of Sciences

Polyion complex （PIC） micelles were spontaneously formed in aqueous solutions through electrostatic interaction between two oppositely charged block copolymers, poly（N-isopropylacrylamide）-b-poly（L-glutamic acid） and poly（N-isopropylacrylamide）-b-poly（L-lysine）. Their controlled synthesis was achieved via the ring opening polymerization of N-carboxyanhydrides （NCA）, ε-benzyloxycarbonyl-L-lysine （Lys（Z）-NCA） or γ-benzyl-L-glutamate （BLG-NCA） with amino-terminated poly（N-isopropylacrylamide） macroinitiator and the subsequent deprotection reaction. The formation of PIC micelles was confirmed by dynamic light scattering and transmission electron microscopy. Turbidimetric characterization suggested that the formed PIC micelles had a concentration-dependent thermosensitivity and their phase transition behaviors could be easily adjusted either by the block length of coplymers or the concentration of micelles.

关键词： Polyion complex Micelles thermosensitivity Poly（amino acid）s.

The Anion Binding Affinity Determines the Strength of Anion Specificities of Thermosensitive Polymers

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Chinese Journal of Polymer Science 2021年第11期39卷 1351-1356页

作者： Lei-Lei Lian Si-Yuan Xu Hai-Yang Yuan Guang-Ming Liu Hefei National Laboratory for Physical Sciences at the Microscale Key Laboratory of Surface and Interface Chemistry and Energy Catalysis of Anhui Higher Education InstitutesDepartment of Chemical PhysicsUniversity of Science and Technology of ChinaHefei 230026China

In this work,we demonstrate that the strength of anion specificities of thermosensitive polymers is determined by the affinity of direct anion binding to the polymers.We have prepared a series of thermosensitive statistical copolymers with distinct thermoresponsive behaviors.The anions can specifically interact with the different types of thermosensitive polymers in very different strengths.A similar strength of specific anion effects on thermoresponsive behaviors can be observed at very different salt concentrations for the different types of thermosensitive polymers.A stronger anion binding to the thermosensitive polymers gives rise to a more obvious anion specificity and vice versa.The work presented here opens up opportunities for the application of ion binding affinity to modulate the strength of ion specificities of thermosensitive polymers.

关键词： Specific ion effect Polyzwitterion Ion-polymer interaction thermosensitivity Ion-pairing

Molecular regulation and genetic control of rice thermal response

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The Crop Journal 2021年第3期9卷 497-505页

作者： Yi Kan Hong-Xuan Lin National Key Laboratory of Plant Molecular Genetics CAS Centre for Excellence in Molecular Plant Sciences and Collaborative Innovation Center of Genetics&DevelopmentShanghai Institute of Plant Physiology and EcologyChinese Academy of SciencesShanghai 200032China University of the Chinese Academy of Sciences Beijing 100049China School of Life Science and Technology ShanghaiTech UniversityShanghai 201210China

Global warming threatens food security.Rice(Oryza sativa L.),a vital food crop,is vulnerable to heat stress,especially at the reproductive stage.Here we summarize putative mechanisms of high-temperature perception(via RNA secondary structure,the phyB gene,and phase separation)and response(membrane fluidity,heat shock factors,heat shock proteins,and ROS(reactive oxygen species)scavenging)in plants.We describe how rice responds to heat stress at different cell-component levels(membrane,endoplasmic reticulum,chloroplasts,and mitochondria)and functional levels(denatured protein elimination,ROS scavenging,stabilization of DNA and RNA,translation,and metabolic flux changes).We list temperature-sensitive genetic male sterility loci available for use in rice hybrid breeding and explain the regulatory mechanisms associated with some of them.Breeding thermotolerant rice species without yield penalties via natural alleles mining and transgenic editing should be the focus of future work.

关键词： Rice Thermotolerance Thermal response thermosensitivity Molecular regulation

Isolation and Progeny Transmission of Non-Temperature-Sensitive MS-H Vaccine Strains of Mycoplasma synoviae from Temperature-Sensitive MS-H-Vaccinated Laying Breeder Hens

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Food and Nutrition Sciences 2023年第7期14卷 579-588页

作者： Victor M. Petrone-Garcia Raquel López-Arellano Inkar Castellanos-Huerta Saeed El-Ashram Ebtesam Al-Olayan Danielle Graham Guillermo Tellez-Isaias Departamento de Ciencias Pecuarias Universidad Nacional Autónoma de México (UNAM) Cuautitlan Izcalli Estado de México México Laboratorio No 5: LEDEFAR Unidad de Investigacion Multidisciplinaria Facultad de Estudios Superi-ores Cuautitlan UNAM Cuautitlan Izcalli Estado de México México Department of Poultry Science University of Arkansas Fayetteville USA Department of Zoology Faculty of Science Kafrelsheikh University Kafr El-Sheikh Egypt Department of Zoology College of Science King Saud University Riyadh Saudi Arabia

This pilot study reports the vertical transmission and reverse thermosensitivity of the MS-H vaccine strain of Mycoplasma synoviae (MS) by RAPD in commercial breeders and their progeny. At two weeks of age, breeders were vaccinated with the ts+ MS-H strain. At 9 weeks of age, an outbreak of infectious synovitis (IS) was detected in the progeny. Tracheal swab samples were collected from breeders at 24, 39, 48, and 70 weeks of age. At 9 weeks, pullets swab from the elbow joints were collected. RAPD was performed on the isolates at 39.5°C, and the same ts- MS-H strains were identified in the breeder hens and their progeny. Tracheal swabs from breeder hens were negative to MS isolation at 37°C and 39.5°C at 24- and 39-weeks. MS isolation was recovered from tracheal swabs from 9/10 and 10/10 breeders at 48- and 70- week. At 9 weeks of age in the progeny, MS was isolated from tracheal swabs of 10/10 from non-IS pullets. MS was isolated from 9/10 joints samples. The isolates from breeder hens and their progeny showed non-significant differences in five antimycoplasmic MIC100 values;otherwise, enrofloxacin presented a significant difference in MIC100 value (p thermosensitivity, pathogenicity, and vertical transmission of the MS-H strain. Consequently, it is crucial to contemplate the danger of reversing pathogenicity and transmission to progeny when applying the MS-H vaccine strain.

关键词： Vertical Transmission thermosensitivity Mycoplasma synoviae RAPD Vax-Safe®

The thermosensitivity and separation efficiency of steroids on silica bead grafted by varied ratio under same length of poly(N-isopropylacrylamide)

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The thermosensitivity and separation efficiency of steroids ...

Synthesis and Swelling Properties of Thermosensitive Hydrogels based on Terpolymerization

中国化学会第29届学术年会

作者： Yanli Liang Linlin Dong Fang Lv Weiwei Meng Rongji Dai Yulin Deng School of Life Science beijing institute of technology

Poly(N-isopropylacrylamide)(PNIPAAm)-modified silica bead with varied grafting density and same length was prepared via atom transfer radical polymerization(ATRP). The grafting ratio of PNIPAAm was ch

关键词： Poly(N-isopropylacrylamide) grafting ratio thermosensitivity column efficiency

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Chinese Chemical Letters 2005年第5期16卷 675-676页

作者： CaiHuaNI XianYuZENG HeHUANG SchoolofChemicalandMaterialEngineering SouthernYangtzeUniversiyWuxi214001 CollegeofMaterialsScienceandEngineering BeijingUniversityofChemicalTechnologyBeijing100029 SchoolofChemistryandMaterialsScience HubeiUniversityWuhan43006

Novel thermosensitive hydrogels based on polymerization of N-isopropyl acrylamide, Sodium acrylate, and diacetone acrylamide were synthesized. The swelling ratio and dynamic swelling were investigated. The results indicated that the hydrogels exhibited high water uptake and themosensitivity. The swelling properties and volume phase transition temperature could be adjusted by contents of the comonomers in the gels.

关键词： Hydrogel thermosensitivity swelling property.

Graft copolymerization of N-isopropylacrylamide with 3-(methacryloxy)propyl trimethoxysilane on ultrafine silica and its application in chromatography separation

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Frontiers of Chemical Science and Engineering 2008年第3期2卷 242-247页

作者： Liping ZHANG Yi ZHU Caihua NI School of Chemical and Material Engineering Jiangnan UniversityWuxi 214122China

Thermosensitive core-shell particles were synthesized through graft copolymerization of N-isopropylacrylamide with[3-(methacryloxy)propyl]trimethoxysilane(MPT)coupled on the surface of ultrafine silica beads.The copolymerization was carried out using polyvinyl alcohol as a surfactant,water and cyclohexanol as mixed solvent,and 2,29-azobis(isobutyronitrile)as an initiator.The effect of surfactant concentration and the composition of the mixed solvent on the graft rate were investigated.The structure of modified silica was confirmed by infrared spectra.Differential scanning calorimetry(DSC)has revealed the thermosensitivity of the particles.The thermosensitive particles were used as packing materials of high performance liquid chromatography(HPLC)columns for separating naphthalene derivatives.Satisfactory separation was obtained by controlling the temperature of the column.In contrast,the packing material of silica-MPT has no such separation efficiency due to the lack of thermosensitivity.The effect of the composition of the mobile phase on the separating efficiency was also investigated.The temperature-controlled separation was effective only when the water content was higher than 90%(v/v)in the water-methanol mobile phase.The mechanism for the temperature-controlled separation is attributed to a polarity change of poly(N-isopropylacrylamide)which undergoes volume phase transition on the silica surface as the temperature increases.

关键词： N-isopropylacrylamide thermosensitivity separation HPLC graft copolymerization

Effects of Chitin Nanocrystal on Solution Behavior and Gelation of Chitosan/β-Glycerophosphate Thermosensitive Hydrogel

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Journal of Donghua University(English Edition) 2018年第6期35卷 446-452页

作者：吴杰公菲菲吉亚丽 State Key Laboratory for Modification of Chemical Fibers and Polymer Materials College of Materials Science and EngineeringDonghua University

The chitosan/β-glycerophosphate( CS/β-GP),a physical hydrogel system with thermosensitive and injectable features combined with biocompatibility and biodegradability, has great potentials as matrices for drug or cell encapsulation and delivery,or as in situ gel-forming materials for tissue repair. Here,the chitin nanocrystal( Chi NC) was introduced into the aforementioned system, and its effects on solution behavior and mechanical properties was investigated. The results showed the incorporation of Chi NC complicated sol-to-gel transition process; a higher loading ratio( 20%) speeded up sol-to-gel transition rate,reduced the solto-gel transition temperature,while still maintained shear-thinning behavior or injectable feature. Moreover,the mechanical properties of gels were significantly enhanced by Chi NC, accompanied by decreased water uptake. The above mentioned behavior favored better applications as injectable tissue-repair implants.

关键词： chitosan β-glycerophosphate chitin nanocrystal hydrogel thermosensitivity injectable