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Feasibility study on heap bioleaching of chalcopyrite

Rare Metals 2013年第5期32卷 524-531页

作者： Bo-Wei Chen Jian-Kang National Engineering Laboratory of Biohydrometallurgy General Research Institute for Nonferrous Metals

Bioleaching of chalcopyrite often encountered the formation of passivation layer, which inhibited the leaching process and resulted in a low leaching rate. This inhibitory effect can be eliminated by thermophilic biole- aching. The industrial test of BioCOP technology based on thermophiles was successfully completed, which confirmed the feasibility of chalcopyrite bioleaching. However, industrial leaching rate of chalcopyrite heap bioleaching is lower. This paper described the development status and industrial test of chalcopyrite heap bioleaching technology. The reasons for the lower efficiency of chalcopyrite heap bioleaching were analyzed. The strategies for successful chalcopyrite heap bioleaching were proposed.

关键词： Chalcopyrite Heap bioleaching thermophiles Pyrite Feasibility

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Biological decomposition of Na_2S_2O_3 into sulfur by a newly isolated facultative thermophilic alkaline desulphuricant strain

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Science China Chemistry 2009年第2期52卷 226-230页

作者： ZHANG JianBin1,ZHANG Tong2,MA Kai2,ZHANG PengYan2,LI Qiang2 & WEI XiongHui1 1 Department of Applied Chemistry,College of Chemistry & Molecular Engineering,Peking University,Beijing 100190,China 2 College of Chemical Engineering,Inner Mongolia University of Technology,Huhhot 010051,China 1. Department of Applied Chemistry College of Chemistry & Molecular Engineering Peking University Beijing 100190 China 2. College of Chemical Engineering Inner Mongolia University of Technology Huhhot 010051 China

A facultative thermophilic alkaline desulphuricant strain named GDJ-3 was isolated from neutral soils,enriched on sulfur synthetic medium,and detected in previous *** and chemotax-onomic analyses and 16s RNA gene sequencing showed that the strain was in good agreement with Alpha proteobacterium sp.(97%) and ochrobactrum sp.(98%).The regenerative processes of the solu-tion containing sulfur compounds(SCS) from the strain through an orthogonal test were investigated to get the optimum regenerative *** results showed that regenerative temperature,air flow,and stirring speed of the agitator were the main three variables influencing the regenerative processes of the *** optimum regenerative efficiency of the SCS from the strain was obtained when tem-perature,air flow,and stirring speed of the agitator were 318.2 K,3.0 L/min,and zero r/min,*** this condition,when the cell concentration of the strain was adjusted to 107/mL,the concentra-tions of Na2S2O3 and Na2S in the SCS decreased from 112.68 g/L to 96.88 g/L and from 0.87 g/L to 0.11 g/L in 9.5 ***,XRD spectrum shows that sulfur was formed in the regeneration *** results suggest that the strain has potential application to the regeneration of the industrial so-lution containing sulfur compounds.

关键词： bio-desulfurization thermophiles optimization biocomposition orthogonal test

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Phylogeny of the industrial relevant,thermophilic genera Myceliophthora and Corynascus

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Fungal Diversity 2012年第1期52卷 197-207页

作者： Joost van den Brink Robert A.Samson Ferry Hagen Teun Boekhout Ronald P.de Vries CBS-KNAW Fungal Biodiversity Centre P.O.Box 851673508 AD UtrechtThe Netherlands

Species of the genus Myceliophthora and its teleomorph Corynascus have attracted increasing interest due to their potential to produce thermostable *** study re-assessed the phylogenetic relationship of 49 isolates of nine species belonging to Myceliophthora and *** species,***,was shown not to belong to the genus Myceliophthora and should be placed in the genus *** other species belonged to two phylogenetic clusters:mesophilic fungi with the type species *** and ***,and thermophilic fungi with ***,*** and *** phylogenetic data provides no clear separation of the two genera Corynascus and *** avoid confusion in future taxonomic studies,it is proposed that all existing Corynascus species be renamed to Myceliophthora,which is the old name and the one more frequently ***,this study identified two groups within the isolates listed as *** and assigned one group(five isolates)to *** based on AFLP analysis and mating *** study provides new insights into the genetic differences within the genus Myceliophthora and will therefore be essential for the interpretation of future genomic and physiological studies of these species.

关键词： Myceliophthora Corynascus thermophiles M.heterothallica M.thermophila.Thermophilic fungi Multigene phylogeny AFLP analysis Mating behavior

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Improving zinc recovery by thermoacidophilic archaeon Acidianus copahuensis using tetrathionate

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Transactions of Nonferrous Metals Society of China 2016年第11期26卷 3004-3014页

作者： Camila CASTRO Edgardo R.DONATI CINDEFI (CCT La Plata-CONICET UNLP) Facultad de Ciencias Exactas Universidad Nacional de La Plata (UNLP)

The attachment and bioleaching experiments were conducted to evaluate the zinc recovery from Hualilan ore by thethermoacidophilic archaeon Acidianus copahuensis. Cells of this species pregrown on tetrathionate showed higher capability ofattachment to the ore than cells pregrown on other energy sources and such attachment seemed to be mediated by the product ofextracellular polymeric substances. A. copahuensis achieved a successful bioleaching of the ore reaching 100% of zinc recoverywhen tetrathionate was added. Simultaneous addition of yeast extract and tetrathionate maintained the zinc extraction at higher *** dissolution kinetics was controlled by chemical reaction in cultures with the external addition of tetrathionate but by thediffusion through a product layer of jarosite in the other cultures.

关键词： bioleaching zinc tetrathionate microbial attachment Acidianus copahuensis thermophiles

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Moorella thermoacetica:A promising cytochrome-and quinone-containing acetogenic bacterium as platform for a CO_(2)-based bioeconomy

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Green Carbon 2023年第1期1卷 2-13页

作者： Florian P.Rosenbaum Volker Müller Molecular Microbiology&Bioenergetics Institute of Molecular BiosciencesJohann Wolfgang Goethe UniversityMax-von-Laue-Str.960438 FrankfurtGermany

The negative effect of fossil-based industrial processes on the environment,especially the contribution to global warming by emitting greenhouse gases such as CO_(2) causes a global threat to ***,technologies are demanded by the society for a sustainable and environmentally friendly *** biotechnological use of sugar-based feedstocks to produce valuable products are in conflict with,for example,food *** order to overcome this issue,waste products such as syngas(H_(2),CO and CO_(2))or CO_(2) taken from the atmosphere are of increasing interest for biotechnological *** bacteria are already used at industrial scale to produce sustainable and environmentally friendly biofuels from syngas.A promising candidate due to its physiological flexibility is the thermophilic acetogen Moorella *** contrast to most acetogens *** is not restricted to one energy conserving *** addition to the Ech complex,cytochromes and quinones may be involved in energy conservation by,for example,DMSO *** extra energy con-served can be used to form highly valuable but energy demanding *** this review we give insights into the physiology of this acetogen,the current state of the art of *** as a platform for biotechnological applications and discuss future perspectives.

关键词： Anaerobes Synthesis gas Bioenergetics thermophiles Wood-Ljungdahl pathway

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Influence of Hot Spring Phages on Community Carbon Metabolism: Win, Lose or Draw?

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Advances in Microbiology 2015年第9期5卷 630-643页

作者： Raymond Kepner Department of Biology Marist College Poughkeepsie New York USA

Abundant virus-like particles were concentrated from large-volume samples from two hot springs. Both addition of viral concentrates and addition of samples induced by addition of mitomycin-C changed patterns of carbon source utilization by hot spring microbial communities. Specific effects of the two treatments depended upon both temperature and incubation period. Increased metabolic capability with greater exposure to free phages, consistent with the view that phages are major lateral transporters of metabolic genes, was observed most clearly in microbes incubated at a temperature lower than that encountered in situ. On the other hand, decreases in the diversity of utilizable C sources upon exposure to phages may have been due to lytic activity in which susceptible bacterial populations were differentially reduced by infective viruses, consistent with the “killing the winner” hypothesis. Treatment of cultures with MC-treated culture extracts, assumed to increase exposure to excised prophages, resulted in higher average metabolic rates after 18 h, but lower rates after 48 h of incubation. With incubation at in situ temperature, this same treatment led to an initial increase in the number of readily utilized C sources, followed by a decrease in community metabolic diversity relative to controls in samples from both hot springs. Thus, treatments designed to increase the interaction between hot spring microbes and either free or newly-excised phages had observable time- and temperature-dependent effects on community metabolism, demonstrating an important, yet complex, ecological role for phages in hot spring waters.

关键词： Hot Springs Virus Prophage thermophiles Carbon Metabolism

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Isolation and Characterization of thermophilic Geobacillus sp. SH-1 Degrading Phenanthrene and Aliphatic Hydrocarbons

Isolation and Characterization of thermophilic Geobacillus s...

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第十次全国环境微生物学术研讨会

作者： Jian Zhang Jian Lu Fang-Bo Yu Lu Zhang Shun-peng Li Biao Shen Key Laboratory of Microbiological Engineering of Agricultural Environment,Ministry of Agriculture,Nanjing Agricultural University,Nanjing,210095,People’s Republic of China

A thermophilic bacterium SH-1 degrading phenanthrene and aliphatic hydrocarbons was isolated from deep oil-well and identified as Geobacillus sp based on the 16S rDNA sequence together with morphological,biochemical and physiological ***-1 could degrade n-alkanes from C12 to C33 and phenanthrene as the sole carbon and energy resource at 45-80℃, with an optimum 70℃.The enzymes of n-alkane degradation were inducible in *** degradation rate of n-alkanes was lower in shaking than without shaking,especially that of C21+ n-alkanes and was reduced with the increasing of shaking *** ladA homologue involved in the degradation of long chain alkanes (C17-C33) in Geobacillus thermodenitrificans NG80-2 was *** partial fragment homologous to alkB gene was found by PCR,the closest relative of which was that of Geobacillus thermoglucosidasius TR2 with only 37.8% identity.

关键词： thermophiles Geobacillus sp. n-Alkanes Phenanthrene Biodegration

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