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International Journal of Oral Science 2015年第4期7卷 232-241页

作者： Hui Xu Ding Bai L-Bruno Ruest Jian Q Feng Yong-Wen Guo Ye Tian Yan Jing Yao He Xiang-Long Han State Key Laboratory of Oral Diseases Department of OrthodonticsWest China Hospital of StomatologySichuan University Department of Biomedical Sciences Baylor College of DentistryTexas A&M University

α-smooth muscle actin （α-SMA） and tenascin-c are stress-induced phenotypic features of myofibroblasts. The expression levels of these two proteins closely correlate with the extracellular mechanical microenvironment. We investigated how the expression of α-SMA and tenascin-c was altered in the periodontal ligament （PDL） under orthodontic loading to indirectly reveal the intrinsic mechanical microenvironment in the PDL. In this study, we demonstrated the synergistic effects of transforming growth factor-β1 （TGF-β1） and mechanical tensile or compressive stress on myofibroblast differentiation from human periodontal ligament cells （hPDLcs）. The hPDLcs under higher tensile or compressive stress significantly increased their levels of α-SMA and tenascin-c compared with those under lower tensile or compressive stress. A similar trend was observed in the tension and compression areas of the PDL under continuous light or heavy orthodontic load in rats. During the time-course analysis of expression, we observed that an increase in α-SMA levels was matched by an increase in tenascin-c levels in the PDL under orthodontic load in vivo. The time-dependent variation of α-SMA and tenascin-c expression in the PDL may indicate the time-dependent variation of intrinsic stress under constant extrinsic loading.

关键词： α-smooth muscle actin mechanical load myofibroblast periodontal ligament tenascin-c transforming growthfactor-β1

Involvement of integrin-activating peptides derived from tenascin-c in colon cancer progression

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World Journal of Gastrointestinal Oncology 2021年第9期13卷 980-994页

作者： Motomichi Fujita Hideo Suzuki Fumio Fukai Department of Molecular Patho-Physiology Tokyo University of ScienceNoda 278-8510ChibaJapan Department of Gastroenterology University of TsukubaTsukuba 305-8575IbarakiJapan

tenascin-c(TNc)is an adhesion modulatory protein present in the extracellular matrix that is highly expressed in several malignancies,including colon *** TNc is considered a negative prognostic factor for cancer patients,the substantial role of the TNc molecule in colorectal carcinogenesis and its malignant progression is poorly *** previously found that TNc has a cryptic functional site and that a TNc peptide containing this site,termed TNIIIA2,can potently and persistently activate *** contrast,the peptide FNIII14,which contains a cryptic bioactive site within the fibronectin molecule,can inactivate *** review presents the role of TNc in the development of colitis-associated colorectal cancer and in the malignant progression of colon cancer,particularly the major involvement of its cryptic functional site *** propose new possible prophylactic and therapeutic strategies based on inhibition of the TNIIIA2-induced beta1-integrin activation by peptide FNIII14.

关键词： tenascin-c TNIIIA2 Beta1-integrin Integrin activation colitis-associated colorectal cancer colon cancer

tenascin-c as a prognostic biomarker in osteosarcoma？

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chinese Medical Journal 2009年第22期122卷 2737-2743页

作者： XIONG Wei NIU Peng-yan ZHU Wen-tao cHEN Jing Department of Orthopaedics Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan Hubei 430030 China Department of Nuclear Medicine Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan Hubei 430030 China

Background Treating metastatic osteosarcoma has been challenged in past decades. Extracelluar matrix （EcM） proteins play an important role in the progression of osteosarcoma as they are pivotal components of the tumor microenvironment. Here, we identified potential genes belonging to the EcM and characterized the roles of these genes in the progression of osteosarcoma and their association with outcomes. Methods Osteosarcoma parental cell line MG63 and its derivative MG63-A1 with a high metastatic potential underwent oligonucleotide microarray analysis. Gene ontology analysis was used to screen deregulated genes between the 2 cell lines which were either upregulated or downregulated by more than 4 fold, particularly focusing on mRNAs encoding extracellular matrix proteins. The expression of resulting candidate genes was then validated by reverse transcription-PcR for mRNA expression as well as Western blotting for protein expression. Immunohistochemistry was performed on 37 osteosarcoma specimens to examine the potential role of the candidate genes in a clinical context. Results Microarray data and gene ontology analysis showed that tenascin-c, a critical component of the EcM, is significantly down-regulated in the highly metastatic cell line MG63-A1 compared with the parental osteosarcoma cell line MG63-wt. This finding was validated at mRNA and protein levels. Immunohistochemical analysis found that tenascin-c is located in the intercellular space in osteosarcoma specimens. Furthermore, low-grade tenascin-c expression （less than 20%） in osteosarcoma specimens was associated with poor survival. conclusions tenascin-c expression level correlates with the survival of osteosarcoma patients. Its biological functional role and underlying molecular mechanisms in the progression of osteosarcoma needs further investigation.

关键词： osteosarcoma metastasis tenascin-c

tenascin-c表达降低抑制成骨细胞分化并促进骨质疏松的机制

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南方医科大学学报 2016年第8期36卷 1117-1122页

作者：陈昱陈梓锋何帆福建省立医院南院骨科福建福州350001 广州市番禺区中心医院创伤烧伤科广东广州511400 杭州市第一人民医院骨科浙江杭州310006

目的探讨tenascin-c在成骨细胞分化中的作用及机制,并分析其表达变化对骨质疏松的调控。方法以免疫印迹法检测去卵巢致骨质疏松的小鼠股骨松质骨的tenascin-c表达变化。体外诱导成骨前体细胞Mc3T3-E1分化,检测瞬时转染tenascin-c的si RN... 详细信息

目的探讨tenascin-c在成骨细胞分化中的作用及机制,并分析其表达变化对骨质疏松的调控。方法以免疫印迹法检测去卵巢致骨质疏松的小鼠股骨松质骨的tenascin-c表达变化。体外诱导成骨前体细胞Mc3T3-E1分化,检测瞬时转染tenascin-c的si RNA后其碱性磷酸酶的活性。以蛋白质相互作用预测软件STRING筛选与tenascin-c可能存在相互作用的蛋白质。在上述候选蛋白中,以免疫沉淀法验证了tenascin-c与Wnt通路内源性抑制蛋白Dickkopf-1(Dkk-1)的相互作用,并以免疫印迹检测tenascin-c对DKK-1的调控。此后进一步采用荧光素酶报告基因系统检测了tenascin-c对Wnt信号通路转录活性的影响。结果去卵巢骨质疏松的小鼠股骨松质中tenascin-c蛋白表达急剧下降。降低tenascin-c的表达能够阻遏Mc3T3-E1向成熟成骨细胞分化,反之,在此基础上过表达β-catenin可逆转前者导致的成骨细胞分化阻滞。tenascin-c结合于DKK-1,敲低tenascin-c可上调DKK-1的表达并降低Wnt通路的转录活性。结论 tenascin-c的下调可能通过减弱对DKK-1的负调控从而抑制Wnt通路的转录活性,进而抑制成骨细胞的分化并介导骨质疏松。

关键词：骨质疏松 tenascin-c 成骨细胞 DKK-1

tenascin-c在瘢痕疙瘩和增生性瘢痕中的基因表达研究

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中华整形外科杂志 2005年第1期21卷 40-43页

作者：韩春茂贺肖洁马奇浙江大学医学院附属第二医院烧伤科杭州310009 浙江大学医学院附属第二医院整形科杭州310009

目的　探讨tenascin c基因在瘢痕疙瘩和增生性瘢痕中的表达。方法　取正常成人皮肤组织RNA ,构建正义、反义tenascin c(Tn c)mRNA探针 ,运用原位杂交技术 ,观测 10例瘢痕疙瘩、10例增生性瘢痕和 5例正常成人皮肤组织中Tn cmRNA的表达。... 详细信息

目的　探讨tenascin c基因在瘢痕疙瘩和增生性瘢痕中的表达。方法　取正常成人皮肤组织RNA ,构建正义、反义tenascin c(Tn c)mRNA探针 ,运用原位杂交技术 ,观测 10例瘢痕疙瘩、10例增生性瘢痕和 5例正常成人皮肤组织中Tn cmRNA的表达。结果　Tn cmRNA在正常皮肤表皮中无表达 ,真皮中表达稀少 ,局限于乳头真皮层的成纤维细胞和皮肤附属器 ;10例瘢痕疙瘩表皮均有表达 ,真皮分布较广 ,如成纤维细胞、血管内皮和皮肤附属器 ;Tn cmRNA在 3例增生性瘢痕表皮表达 ,7例无表达 ,真皮中表达与瘢痕疙瘩相同但较弱 ,比正常皮肤增多 ,但差异无显著性。结论　tenascin cmRNA在瘢痕疙瘩表皮和真皮中有高表达。

关键词： tenascin-c 瘢痕疙瘩增生性瘢痕基因表达

tenascin-c促进肿瘤转移的分子机制研究进展

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生物医学工程学杂志 2015年第1期32卷 240-244页

作者：农云宏白浪唐红四川大学华西医院感染性疾病中心成都610041 四川大学生物治疗国家重点实验室感染性疾病研究室成都610041

tenascin-c(TNc)是一种细胞外基质糖蛋白,在胚胎组织和肿瘤组织中高表达,而在成熟组织中不表达或低表达。TNc在肿瘤转移过程中涉及多种复杂的信号通路,通过与syndecan-4、整合素α5β1、基质金属蛋白酶(MMPs)、血管内皮生长因子(VEGF)... 详细信息

tenascin-c(TNc)是一种细胞外基质糖蛋白,在胚胎组织和肿瘤组织中高表达,而在成熟组织中不表达或低表达。TNc在肿瘤转移过程中涉及多种复杂的信号通路,通过与syndecan-4、整合素α5β1、基质金属蛋白酶(MMPs)、血管内皮生长因子(VEGF)等相互作用,对FAK和RhoA信号通路、Wnt信号通路及Notch信号通路进行调节,从而影响上皮间质转化、肿瘤细胞黏附、侵袭、增殖和血管生成,增强多种肿瘤侵袭和转移的能力。随着研究的深入,TNc在某些恶性肿瘤中可以用于评价患者预后或者作为转移的标志。

关键词： tenascin-c 肿瘤转移肿瘤细胞黏附肿瘤侵袭肿瘤血管生成信号通路

tenascin-c分子中重组Fibronectin各片段对胎鼠脊髓培养神经元的不同作用

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解剖学报 1997年第4期28卷 369-373,I007页

作者：杨大莉黄文晋鲍璇鞠躬第四军医大学神经科学研究所

用小鼠重组ｔｅｎａｓｃｉｎ－Ｃ（ＴＮ－Ｃ）结构域中类似Ⅲ型ｆｉｂｒｏｎｅｃｔｉｎ（ＦＮ）的不同重复片段，分别作为基质或培养液成分，研究其对培养的胚胎小鼠脊髓神经突起生长、粘附力及神经元活性的影响。结果显示：无论作为基... 详细信息

用小鼠重组ｔｅｎａｓｃｉｎ－Ｃ（ＴＮ－Ｃ）结构域中类似Ⅲ型ｆｉｂｒｏｎｅｃｔｉｎ（ＦＮ）的不同重复片段，分别作为基质或培养液成分，研究其对培养的胚胎小鼠脊髓神经突起生长、粘附力及神经元活性的影响。结果显示：无论作为基质还是培养液成分，ＦＮ６－８对神经突起生长及神经元活性均有促进作用；ＦＮ３－５对神经突起生长及神经元活性均有抑制作用。ＴＮ－Ｃ促进神经突起生长的功能区在ＦＮ６－８，ＦＮ３－５介导了神经元与ＴＮ－Ｃ的粘附。

关键词： tenascin-c 重组片段神经元培养突起生长

Qingre Quyu Granule(清热祛瘀颗粒)Stabilizes Plaques through Inhibiting the Expression of tenascin-c in Patients with Severe carotid Stenosis

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chinese Journal of Integrative Medicine 2015年第5期21卷 339-345页

作者：王一程文立王勇彭婧嫔袁杰陈莉潘琳李鸿郭健 Beijing University of chinese Medicine Fushun Hospital of Traditional chinese Medicine Department of National Integrative Medicine center for cardiovascular Disease China-Japan Friendship Hospital clinical Research Institute China-Japan Friendship Hospital

Objective： To investigate the therapeutic effects of Qingre Quyu Granule （请热祛瘀颗粒, QQG） on the patients with severe carotid stenosis, and to explore the mechanism of it. Methods： Ninety-six patients with severe carotid stenosis were enrolled in the study and were classified into a QQG group （n=48） and a control group （n=48） randomly using consecutively numbered envelopes. The patients in the QQG group were given QQG and Western medicine, those in the control group were given Western medicine merely, the course of treatment was 16 weeks. All patients went through endarterectomy after treatment. Plaques were subjected to the analysis of cD3, cD68, soluble intercellular adhesion molecule 1 （IcAM-1）, matrix metalloprotease-9 （MMP-9）, cD40L, tenascin-c, and collagen content lipid content by immunohistochemistry or polarized light analysis. Results： By the end of experiment, the expressions of cD3, cD68, IcAM-1, MMP9, cD40L and tenascin-c on the plaques were statistically significant lower in the QQG group compared with the control group （P〈0.01）, The lipid content of the plaque was also significantly lower in the QQG group compared with the control group （P〈O.01）, The interstitial collagen in the tissue sections of the plaques was also significantly higher in the QQG group in comparison with the control group （P〈0.01）. conclusion： QQG could stabilize carotid artery plaques through inhibiting pro-inflammation factors and restraining the tenascin-c and MMP9 pathway.

关键词： vulnerable atherosclerotic plaque cD40L matrix metalloprotease-9 tenascin-c chinesemedicine Qingre Quyue Granule

tenascin-c在人原发性肝细胞癌浸润、转移和血管生成中的作用

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华中科技大学学报（医学版） 2012年第3期41卷 278-283页

作者：尹传昌廖晓峰陈雯郭跃清易继林李兴睿华中科技大学同济医学院附属同济医院普外科武汉430030 华中科技大学同济医学院附属同济医院妇产科武汉430030

目的研究tenascin-c在原发性肝细胞癌(Hcc)中的表达,探讨tenascin-c与Hcc浸润、转移和血管生成的关系。方法采用免疫组织化学方法检测54例Hcc、32例肝硬化、17例正常肝组织中tenascin-c的表达情况以及微血管密度(MVD);采用逆转录聚合酶... 详细信息

目的研究tenascin-c在原发性肝细胞癌(Hcc)中的表达,探讨tenascin-c与Hcc浸润、转移和血管生成的关系。方法采用免疫组织化学方法检测54例Hcc、32例肝硬化、17例正常肝组织中tenascin-c的表达情况以及微血管密度(MVD);采用逆转录聚合酶链反应(RT-PcR)方法检测tenascin-c mRNA,并结合临床病理指标进行分析。结果 tenascin-c在Hcc、肝硬化和正常肝脏组织中的阳性例数分别为34、6和0,其差异具有统计学意义(χ2=14.06,Ptenascin-c在Hcc组织内的表达强度明显高于肝硬化组织[(162.15±9.77)vs.(149.24±8.25),t=2.192,Pcc组织tenascin-c mRNA的表达强度明显高于肝硬化组织[(0.593±0.110)vs.(0.138±0.089),t=2.894,Pcc组织中tenascin-c表达量明显高于无浸润者(t=2.248,Pcc属Ⅲ～Ⅳ级的tenascin-c含量明显高于Ⅰ～Ⅱ级者(t=2.698,Pcc有转移的Te-nascin-c含量明显高于无转移者(t=2.785,Pcc组织中tenascin-c表达与微血管密度(MVD)呈正相关(r=0.68,Pcc患者在随访期间(3～30个月,平均11.5个月),tenascin-c阴性表达的患者中位生存时间长于tenascin-c阳性表达的患者[(22.01±6.95)月vs.(15.08±7.06)月,Ptenascin-c对Hcc的浸润、转移和血管生成起着重要作用。tenascin-c和MVD可以作为预测Hcc浸润、转移的生物学指标。

关键词： tenascin-c 原发性肝细胞癌微血管密度肿瘤转移

tenascin-c在慢性免疫性心肌炎小鼠心室重构中的作用

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解放军医学杂志 2009年第8期34卷 963-966页

作者：吴辉叶明唐其柱丁家望李书国吕志阳熊艳李松李莉杨俊湖北宜昌三峡大学第一临床医学院宜昌市中心人民医院心血管内科443003 宜昌市第一人民医院神经内科武汉大学人民医院心内科

目的探讨基质细胞蛋白tenascin-c在慢性免疫性心肌炎小鼠心室重构中的作用。方法SPF级BALB/c小鼠75只,随机分为实验组(45只)和对照组(30只)。以心肌肌球蛋白重链614-629位多肽与完全弗氏佐剂的混合液免疫实验组小鼠建立实验性自身免疫... 详细信息

目的探讨基质细胞蛋白tenascin-c在慢性免疫性心肌炎小鼠心室重构中的作用。方法SPF级BALB/c小鼠75只,随机分为实验组(45只)和对照组(30只)。以心肌肌球蛋白重链614-629位多肽与完全弗氏佐剂的混合液免疫实验组小鼠建立实验性自身免疫性心肌炎(EAM)模型,对照组注射相同量磷酸盐缓冲液与完全弗氏佐剂的混合液。分别在不同时间点(21、75、120d)观察小鼠心肌的病理性重构情况,采用Western blotting检测心肌tenascin-c的表达,放射免疫法测定血清Ⅲ型胶原氨基末端肽(PⅢNP)含量,并分析两者之间的相关性。结果21d时,实验组小鼠心肌炎性浸润明显,间质出现少量胶原沉积;至75、120d时,实验组小鼠心肌炎性浸润显著减少,间质胶原沉积更加明显。实验组在各时间点血清PⅢNP含量及心肌tenascin-c蛋白表达均显著高于对照组(Ptenascin-c蛋白的表达与血清PⅢNP含量呈正相关(r=0.893,Ptenascin-c参与了心肌炎小鼠心肌纤维化及心室重构的进展,可能在心肌疾病的发生发展中具有重要作用。

关键词：自身免疫心肌炎 tenascin-c 心室重构