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Effects on the STAT3-shRNA in Non-Small-Cell Lung Cancer Therapy: Design, Induction of Apoptosis, and Conjugation with Chitosan-Based Gene Vectors

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Journal of Ocean University of China 2021年第5期20卷 1097-1108页

作者： ZHANG Wangwang ZHANG Yan JIANG Zhiwen SUN Le WANG Litong GU Zhiyang LI Wenya GUO Lili CHEN Xiaotong ZHANG Haibin HAN Baoqin CHANG Jing College of Marine Life Science Ocean University of ChinaQingdao 266003China Laboratory for Marine Drugs and Bioproducts Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266235China

STAT3 plays a particularly important role in several cancer-related signal transduction *** STAT3 via RNA interference or small molecule inhibitors induces the apoptosis of tumor cells,thereby inhibiting the growth of the *** this study,short-hairpin RNA sequences targeting the STAT3 genes were designed,synthesized,and then connected to pGPU6/GFP/Neo plasmids as the shRNA-expression *** expression of STAT3-shRNA was analyzed by real-time PCR,western blotting,and cell apoptosis assay to study the growth and apoptosis of the ***,the effect of STAT3 knockdown on the NCI-H1650 cells was studied in a tumor mouse *** results revealed that,after an in vitro transfection,the proliferation of NCI-H1650 cells was inhibited,and the cells were induced to *** mRNA and protein expression levels of STAT3 were downregulated in the STAT3-shRNA *** vivo,the tumor mass and volume in the STAT3-shRNA group were significantly lower than in the other two *** the in vivo and in vitro results demonstrated a long-period inhibiting effect on NSCLC,especially in vivo,when the tumor inhibition rate could reach 50%in the STAT3-shRNA group,which is an exciting ***,the study of the conjugation of STAT3-shRNA and chitosan-based vectors revealed that they could be combined steadily with good cytocompati-bility and transfection *** results together provide convincing evidence for the application of STAT3-shRNA used in the treatment of non-small lung cancer,which could be a promoting prospect for the development of gene therapy.

关键词： signal transducer and activator of transcription 3 short-hairpin RNA target downregulation chitosan-based vectors gene therapy

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