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tapered optical fiber DNA biosensor for detecting Leptospira DNA

Asian Pacific Journal of Tropical Medicine 2023年第3期16卷 119-128页

作者： Jia-Yong Lam Mohd Hanif Yaacob Hui-Yee Chee Department of Medical Microbiology Faculty of Medicine and Health SciencesUniversiti Putra Malaysia43400 UPM SerdangSelangorMalaysia Department of Computer and Communication Systems Engineering Faculty of EngineeringUniversiti Putra Malaysia43400 UPM SerdangSelangorMalaysia Wireless and Photonics Networks Research Centre(WiPNET) Universiti Putra Malaysia43400 UPM SerdangSelangorMalaysia

Objective: To establish a DNA detection platform based on a tapered optical fiber to detect Leptospira DNA by targeting the leptospiral secY ***: The biosensor works on the principle of light propagating in the special geometry of the optical fiber tapered from a waist diameter of 125 to 12 μm. The fiber surface was functionalized through a cascade of chemical treatments and the immobilization of a DNA capture probe targeting the secY gene. The presence of the target DNA was determined from the wavelength shift in the optical transmission ***: The biosensor demonstrated good sensitivity, detecting Leptospira DNA at 0.001 ng/μL, and was selective for Leptospira DNA without cross-reactivity with non-leptospiral microorganisms. The biosensor specifically detected DNA that was specifically amplified through the loop-mediated isothermal amplification ***: These findings warrant the potential of this platform to be developed as a novel alternative approach to diagnose leptospirosis.

关键词： DNA biosensor tapered optical fiber Leptospirosis Leptospira

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Temperature sensor based on a single-mode tapered optical fiber

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Journal of Shanghai University(English Edition) 2011年第2期15卷 101-104页

作者：朱姗邢洁雯庞拂飞王廷云 Key Laboratory of Specialty fiber Optics and optical Access Networks School of Communication and Information Engineering Shanghai University

A temperature sensor is demonstrated and fabricated by coating the single-mode tapered optical fiber with temperature-sensitive silicone rubber. It works on the change of the evanescent fields in the tapered optical fiber. Small changes in the refractive index of coating film greatly influence the power of evanescent fields, which modulate the transmission optical power in the waist region. The range of temperature measured is from -20℃ to 70 ℃. The results show that the temperature sensor has high temperature sensitivity （0.012 mW/℃） and good repeatability.

关键词： temperature sensor tapered optical fiber silicone rubber

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Single-Mode Modified tapered fiber Structure Functionalized With GO-PVA Composite Layer for Relative Humidity Sensing

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Photonic Sensors 2021年第3期11卷 314-324页

作者： Aneez Syuhada Muhammad Salleh Shamsudin Suzairi Daud Ganesan Krishnan Sulaiman Wadi Harun Muhammad Safwan Abd.Aziz Department of Physics Faculty of ScienceUniversiti Teknologi MalaysiaJohor Bahru81310Malaysia School of Engineering Faculty of Engineering and Physical SciencesUniversity of Southampton MalaysiaIskandar Puteri79200Malaysia Department of Electrical Engineering University of MalayaKuala Lumpur50603Malaysia Laser Center Ibnu-Sina Institute for Scientific and Industrial Research(ISI-SIR)Universiti Teknologi MalaysiaJohor Bahru81310Malaysia

A sensitive tapered optical fiber sensor incorporating graphene oxide(GO)and polyvinyl alcohol(PVA)composite film for the rapid measurement of changes in relative humidity was proposed and experimentally *** sensing principle was based on the intensity modulation of the transmitted light induced by the refractive index changes of the sensitive *** sensing region was obtained by tapering a section of single-mode optical fiber(SMF)from its original 125µm diameter down to 9.03µ*** tapered structure was then modified through deposition of GO/PVA nanocomposites by using the dip-coating *** field emission scanning electron microscope(FESEM)and Raman spectroscopy were used to characterize the structure of the composite *** evidenced by a Fourier transform infrared spectroscopy(FTIR)analysis,the presence of oxygen functional groups(such as−OH and COOH)on the GO structure enabled the attachment of PVA molecules through hydrogen bonding and strong adhesion between GO/PVA *** performance of the sensor was tested over a wide range(20%RH to 99.9%RH)of relative *** sensor showed a good response with its signal increasing linearly with the surrounding *** tapered optical fiber sensor with the coating of GO/0.3g PVA achieved the highest sensitivity[0.5290RH(%)].The stability,repeatability,reversibility,as well as response time of the designated sensor were also measured and analyzed.

关键词： Humidity sensor tapered optical fiber Graphene Oxide PVA

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