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synergistic action of non-solvent induced phase separation in preparation of poly(vinyl butyral) hollow fiber membrane via thermally induced phase separation

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Journal of Central South University 2014年第6期21卷 2184-2190页

作者：邱运仁漆静韦玉清 School of Chemistry and Chemical Engineering Central South University

A systematic study of air gap distance effects on the structure and properties of poly(vinyl butyral)hollow fiber membrane via thermally induced phase separation(TIPS)has been carried *** results show that the hollow fiber membrane prepared at air gap zero has no skin layer; the pore size near the outer surface is larger than that near the inner surface; and the special pore channel-like structure near the outer surface is formed,which is quite different with the typical sponge-like structure caused by TIPS and the finger-like structure caused by non-solvent induced phase separation(NIPS),because of the synergistic action of non-solvent induced phase separation at air gap *** pore size gradually decreases from outer surface layer to the intermediate layer,but increases gradually from intermediate layer to the inner surface *** the increase of air gap distance,the pore size near the outer surface gets smaller and a dense skin layer is formed,and the pore size gradually increases from the outer surface layer to the inner surface *** permeability of the hollow fiber membrane decreases with air gap distance,the water permeability decreases sharply from 45.50×10-7 to 4.52×10-7 m3/(m2·s·kPa)as air gap increases from 0 to 10 mm at take-up speed of 0.236 m/s,further decreases from 4.52×10-7 to 1.00×10-8 m3/(m2·s·kPa)as the air gap increases from 10 to 40 *** the breaking strength and the elongation increase with the increase of air gap *** breaking strength increases from 2.25 MPa to 4.19 MPa and the elongation increases from 33.9% to 132.6% as air gap increases from 0 mm to 40 mm at take-up speed 0.236 m/s.

关键词： thermally induced phase separation hollow fiber membranes synergistic action hydrophilic membrane

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synergistic action of CBP and Cameo2 on the Cellular Lutein Uptake in Silkworm,Bombyx mori

Synergistic action of CBP and Cameo2 on the Cellular Lutein ...

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中国遗传学会第九次全国会员代表大会暨学术研讨会

作者：王炜 Mao-Hua Huang Xiao-Long Dong Chun-Li Chai Cai-Xia Pan Hui Tang Yan-Hong Chen Fang-Yin Dai 潘敏慧鲁成 State Key Laboratory of Silkworm Genome Biology Southwest University Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry Southwest University

The yellow-red color cocoon formation of Bombyx mori is due to the selective delivery of carotenoids from midgut lumen into silk gland,and is thought to be mediated by specific cellular carotenoids carrier *** researches have determined that CBP and Cameo2 are the products of the yellow blood(Y) gene and the yellow cocoon(C) gene of silkworm,*** the yellow cocoon variety,they are mainly responsible for the selective delivery of lutein into middle *** CBP and Cameo2 are expressed in the same tissues of the larval body of the C allele strain with the background of the Y allele and the Y gene is also involve in the absorption of other carotenoids such asβ-carotene from the midgut lumen into midgut mucosal cells,speculating that the selective delivery of carotenoids to specific tissues might requires the combined action of CBP and *** order to prove the postulation and make clear their respective contribution in the cellular transport of *** interrelation between the type and content of carotenoids and the gene expressing of CBP and Cameo2 in different tissues from four silkworm varieties producing different color cocoons were *** the same time,the contribution of CBP and Cameo2 in cellular carotenoids uptake was researched in cell *** results show that CBP and Cameo2 genes were expressed simultaneously only in the tissues accumulating large amounts of *** co-expressing CBP and Cameo2 can absorb higher lutein than the control group and the cells expressing single protein group,differences are very significant(Paction between CBP and Cameo2 in the area near the plas

关键词： Bombyx mori cocoon color synergistic action CBP Cameo2 lutein β-carotene

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synergistic action of CBP and Cameo2 on the Cellular Lutein Uptake in Silkworm,Bombyx mori

Synergistic action of CBP and Cameo2 on the Cellular Lutein ...

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第十届家（柞）蚕遗传育种及良种繁育学术研讨会

作者： Wei Wang Mao-Hua Huang Xiao-Long Dong Chun-Li Chai Cai-Xia Pan Hui Tang Yan-Hong Chen Fang-Yin Dai Min-Hui Pan Cheng Lu State Key Laboratory of Silkworm Genome Biology Southwest University Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry Southwest University

The yellow-red color cocoon formation of Bombyx mori is due to the selective delivery of carotenoids from midgut lumen into silk gland, and is thought to be mediated by specific cellular carotenoids carrier proteins. Previous researches have determined that CBP and Cameo2 are the products of the yellow blood（Y） gene and the yellow cocoon（C） gene of silkworm, respectively. In the yellow cocoon variety, they are mainly responsible for the selective delivery of lutein into middle silkgland. As CBP and Cameo2 are expressed in the same tissues of the larval body of the C allele strain with the background of the Y allele and the Y gene is also involve in the absorption of other carotenoids such asβ-carotene from the midgut lumen into midgut mucosal cells, speculating that the selective delivery of carotenoids to specific tissues might requires the combined action of CBP and Cameo2. In order to prove the postulation and make clear their respective contribution in the cellular transport of carotenoids. The interrelation between the type and content of carotenoids and the gene expressing of CBP and Cameo2 in different tissues from four silkworm varieties producing different color cocoons were investigated. As the same time, the contribution of CBP and Cameo2 in cellular carotenoids uptake was researched in cell lines. The results show that CBP and Cameo2 genes were expressed simultaneously only in the tissues accumulating large amounts of lutein. Cells co-expressing CBP and Cameo2 can absorb higher lutein than the control group and the cells expressing single protein group, differences are very significant（P＜0.01）, and the cellular uptake of lutein was concentration-dependent and saturation. Furthermore Cameo2 and CBP were located in different region of the cells, the former was localized at the plasma membrane, and the latter in the entire cells. Bimolecular fluorescence complementation（BiFC） analysis showed that there was potential interaction between CBP and Cameo2 in the

关键词： Bombyx mori cocoon color synergistic action CBP Cameo2 lutein β-carotene

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Combination of telomerase antisense oligonucleotides simultaneously targeting hTR and hTERT produces synergism of inhibition of telomerase activity and growth in human colon cancer cell line

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World Journal of Gastroenterology 2005年第6期11卷 785-790页

作者： Xiao-HuaFu Jian-SongZhang NaZhang Yang-DeZhang MedicalCollege HunanNormalUniversityChangsha410006HunanProvinceChina NationalHepatobiliaryandEntericSurgeryResearchCenterofMinistryofHealth China

AIM: To investigate synergism of inhibition of telomerase activity and proliferation of human colon cancer cells by combination of telomerase antisense oligonucleotides (ASODNs) simultaneously targeting human telomerase RNA (hTR) and human telomerase reverse transcriptase (hTERT) in vitro. METHODS: ASODN of hTR and ASODN of hTERT were transfected into human colon cancer SW480 cells by liposomal transfection reagents. Telomerase activity of SW480 cells was examined using telomeric repeat amplification protocol (TRAP)-enzyme-linked immunosorbent assay (PCR-ELISA). Proliferation activity of SW480 cells was tested by methyl thiazolyl tetrazolium assay. Apoptosis and cell cycle were analyzed by flow cytometry. RESULTS: The telomerase activity and cell survival rate in SW480 cells transfected with 0.2 μmol/L of ASODN of hTR or ASODN of hTERT for 24-72 h were significantly decreased in a time-dependent manner compared with those after treatment with sense oligonucleotides and untreated (telomerase activity: 24 h, 73%, 74% vs99%, 98%; 48 h, 61%, 55% vs98%, 99%; 72 h, 41%, 37% vs 99%, 97%; P<0.01; cell survival rate: 24 h, 88%, 86% vs594%, 98%; 48 h, 49%, 47% vs94%, 97%; 72 h, 44%, 42% vs92%, 96%; P<0.01). Moreover, the telomerase activity and the cell survival rate in SW480 cells treated by the combination of telomerase anti-hTR and anti-hTERT were more significantly suppressed than single anti-hTR or anti-hTERT (telomerase activity: 24 h, 59% vs 73%, 74%; 48 h, 43% vs61%, 55%; 72 h, 18% vs41%, 37%; P<0.01; cell survival rate: 24 h, 64% vs88%, 86%; 48 h, 37% vs49%, 47%; 72 h, 25% vs44%, 42%; P<0.01). Meanwhile, the apoptosis rates in the combination group were markedly increased compared with those in the single group (24 h, 18.0% vs7.2%, 7.4%; 48 h, 23.0% vs13.0%, 14.0%; 72 h, 28.6% vs 13.2%, 13.75; P<0.01). Cells in combination group were arrested at G0/G1 phase. CONCLUSION: Telomerase anti-hRT and anti-hTERT suppress telomerase activity, and inhibit growth of human colon

关键词： Telomerase reverse transcriptase Telomerase RNA Antisense oligonucleotides synergistic action Colon cancer

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Imaging-Guided Cancer Therapy Based on Multifunctional Magnetic Nanoparticles

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Chinese Journal of Chemistry 2023年第19期41卷 2557-2573页

作者： Ran Ma Yanglong Hou Shuren Wang Beijing Key Laboratory of Magnetoelectric Materials and Devices School of Materials Science and EngineeringBeijing Innovation Centre for Engineering Science and Advanced TechnologyPeking UniversityBeijing100871 China

In recent years,magnetic nanoparticles(MNPs)have received great attention within the field of biomedicine,especially for cancer *** is because MNPs have many excellent physical and chemical properties to provide sufficient imaging information along with satisfactory therapeutic ***,by virtue of various modification strategies,the obtained multifunctional MNPs can further achieve synergized multimodal cancer theranostic,which is worthy of further *** this review,we summarize the recent developments in imaging-guided strategies and synergistic cancer therapy based on multifunctional ***,we discuss the challenge and perspective of the next generation of MNPs-based imaging-guided cancer therapy,hoping to provide guidance in potential applications.

关键词： Cancer Cancer therapy Modal imaging Magnetic nanoparticles synergistic action Nanotechnology Synthesis design Drugdelivery Imagingagents

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The Influence of Seasonal Snow on Soil Thermal and Water Dynamics under Different Vegetation Covers in a Permafrost Region

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Journal of Mountain Science 2014年第3期11卷 727-745页

作者： HANG Juan WANG Gen-xu GAO Yong-heng WANG Yi-bo College of Earth and Environmental Sciences Lanzhou University Institute of Mountain Hazards and Environment Chinese Academy of Sciences

Seasonal snow is one of the most important influences on the development and distribution of permafrost and the hydrothermal regime in surface soil. Alpine meadow, which constitutes the main land type in permafrost regions of the Qinghai-Tibet Plateau, was selected to study the influence of seasonal snow on the temperature and moisture in active soil layers under different vegetation coverage. Monitoring sites for soil moisture and temperature were constructed to observe the hydrothermal processes in active soil layers under different vegetation cover with seasonal snow cover variation for three years from 2010 to 2012. Differences in soil temperature and moisture in areas of diverse vegetation coverage with varying levels of snow cover were analyzed using active soil layer water and temperature indices. The results indicated that snow cover greatly influenced the hydrothermal dynamics of the active soil layer in alpine meadows. In the snow manipulation experiment with a snow depth greater than 15 cm, the snow cover postponed both the freeze-fall and thawrise onset times of soil temperature and moisture in alpine LC(lower vegetation coverage) meadows and of soil moisture in alpine HC(higher vegetation coverage) meadows; however, the opposite response occurred for soil temperatures of alpine HC meadows,where the entire melting period was extended by advancing the thaw-rise and delaying the freeze-fall onset time of the soil temperature. Snow cover resulted in a decreased amplitude and rate of variation in soil temperature, for both alpine HC meadows and alpine LC meadows, whereas the distinct influence of snow cover on the amplitude and rate of soil moisture variation occurred at different soil layers with different vegetation coverages. Snow cover increased the soil moisture of alpine grasslands during thawing periods. The results confirmed that the annual hydrothermal dynamics of active layers in permafrost were subject to the synergistic actions of both snow cover

关键词： Permafrost Snow cover Vegetationcover Active soil layer Hydrothermal dynamics synergistic action

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Latest research progress of marine microbiological corrosion and biofouling, and new approaches of marine anti-corrosion and anti-fouling

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Bioactive Materials 2019年第1期4卷 189-195页

作者： Yangfan Li Chengyun Ning School of Materials Science and Engineering South China University of TechnologyChina

Marine resources and industry have become one of the most important pillars in economic development all over the ***,corrosion of materials is always the most serious problem to the infrastructure and equipment served in marine *** have found that microbiologically influenced corrosion(MIC)and marine bio-fouling are two main mechanisms of marine corrosions due to the complicated marine environment and marine *** article summarized the latest research progress about these two mechanisms and indicated that both MIC and marine bio-fouling are closely related to the biofilms on material surfaces formed by the marine microorganisms and their *** a result,to prevent the occurrence of MIC and bio-fouling,it is important to control the microorganisms in biofilms or prevent the adhesion and formation of *** traditional method of using chemical bactericide or antifoulant faces the problems of pollution and microorganism *** article introduced four research approaches about the new tendency of applying new materials and technologies to cooperate with traditional chemicals to achieve better and longer effects with lower environment pollution through synergistic ***,some future research tendencies were proposed for whole marine anti-corrosion and anti-fouling areas.

关键词： Microbiologically influenced corrosion Marine bio-fouling Biofilms synergistic action Anti-Corrosion

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Experimental study on low temperature performance of polymer drilling mud agents

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Global Geology 2009年第1期12卷 32-35页

作者： ZHAN Jiajia XU Huiwen CAI Hongliang College of Construction Engineering Jilin University Changchun 130026 China China Water Northeastern Investigation Design and Research Co. Ltd Changchun 130061 China

Drilling fluid additives has a major impact on rheology behavior and other performances of the drilling *** key to low temperature drilling fluids is choosing antifreeze and polymer agent which have a strong ability in resisting low *** the basis of determining glycol as antifreeze agent,the low-temperature-performance and capacities of anti-collapse of regular validity mud agents have been studied,the mechanism of anti-caving of the partially hydrolyzed polyacrylamide(PHPA),polypropylene potassium(KPA) and PAC-141 polymer additives have been also analyzed.A pilot study of compatibility between ethylene glycol and the polymers has educed that the polymers,ethylene glycol and inorganic salt are compatible,and they can significantly reduce the freezing point of drilling ***-collapse ability of the drilling mud is the results of synergistic action among the agents.

关键词： polymer low temperature performance antifreeze agent mechanism of anti-caving synergistic action

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