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Real-time in situ observation of P53-mediated cascade activation of apoptotic pathways with nucleic acid multicolor fluorescent probes based on symmetrical gold nanostars

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Nano Research 2023年第4期16卷 5391-5400页

作者： Chenbiao Li Peifang Chen Xiaoyuan Ma Xichi Lin Shan Xu Sobia Niazi Zhouping Wang State Key Laboratory of Food Science and Technology Jiangnan UniversityWuxi 214122China School of Food Science and Technology Jiangnan UniversityWuxi 214122China International Joint Laboratory on Food Safety Jiangnan UniversityWuxi 214122China Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province Jiangnan UniversityWuxi 214122China

T-2 toxin,one of the most dangerous natural pollutants,induces apoptosis through multiple ***,P53 mediated apoptosis pathway,an important collection of molecules,plays a key role in cell vital ***-time monitoring of upstream and downstream activation relationships of P53 mRNA,Bax mRNA,and cytochrome c(Cyt c)in signaling pathways is of great significance for understanding the apoptotic machinery in human *** this work,a novel nucleic acid multicolor fluorescent probe,based on silica-coated symmetric gold nanostars(S-AuNSs@SiO_(2)),was developed for highly sensitive in situ real-time imaging of P53 mRNA,Bax mRNA,and Cyt c during T-2 toxin-induced *** nucleic acid chains modified with carboxyl groups were modified on the surface of S-AuNSs@SiO_(2)by amide *** complementary chains of targeted mRNA and the aptamer of targeted Cyt c were modified with different fluorophores,respectively,and successfully hybridized on S-AuNSs@SiO_(2)*** targets were present,the fluorescent chains bound to the targets and detached from the material,resulting in the quenched fluorescence being *** probes based on S-AuNSs showed excellent performance is partly ascribed to the presence of 20 symmetric“hot spots”.Notably,the amide-bonded probe exhibited excellent anti-interference capability against biological agents(nucleases and biothiols).During the real-time fluorescence imaging of T-2 toxin-induced apoptosis,the corresponding fluorescence signals of P53 mRNA,Bax mRNA,and Cyt c were observed ***,S-AuNSs@SiO_(2)probe not only provides a novel tool for real-time monitoring of apoptosis pathways cascade but also has considerable potential in disease diagnosis and pharmaceutical medical.

关键词： nucleic acid multicolor fluorescent probe symmetric gold nanostars T-2 toxin P53 mediated apoptosis pathway living cells imaging

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