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Phorbol-induced surface expression of NR2A subunit homologues in HEK293 cells

Acta Pharmacologica Sinica 2006年第12期27卷 1580-1585页

作者： Chan-ying ZHENG~2 Xiu-juan YANG~3 Zhan-yan FU~4 Jian-hong LUO~(2,5)2 Department of Neurobiology,Zhejiang University School of Medicine,Hangzhou 310031,China 3 Institute of Neuroscience,Shanghai Institutes for Biological Sciences,the Chinese Academy of Sciences,Shanghai 200031,China 4 Department of Physiology and Biophysics,Georgetown University School of Medicine Washington 20057,USA DepartmentofNeurobiology ZhejiangUniversitySchoolofMedicineHangzhou310031China InstituteofNeuroscience ShanghaiInstitutesforBiologicalSciencestheChineseAcademyofSciencesShanghai200031China

Aim:N-methyl-D-aspartate receptors(NMDAR)are heteromeric complexes pri-marily assembled from NR1 and NR2 *** normal conditions,NR2 sub-units assemble into homodimers in the endoplasmic reticulum(ER).Thesehomodimers remain in the ER until they coassemble with NR1 dimers and aretrafficked to the cell ***,it still remains unclear whether functionalhomomeric NMDAR exist in physiological or pathological ***:We transfected GFP-NR2A alone into HEK293 cells,treated the cells with PKCactivator 12-myristate-13 acetate(PMA),and then detected surface NR2A sub-units with a live cell immunostaining *** also used a series of NR2Amutants with a partial deletion of its C-terminus to identify the regions that areinvolved in the PMA-mediated surface expression of NR2A ***:NR2A subunits were expressed on the cell membrane after incubation with PMA(200 nmol/L,30 min),although no functional NMDA channels were detected afterPMA-induced membrane *** with an ER marker alsorevealed that NR2A subunits were exported from the ER after PMA ***,the deletion of amino acids between 1149-1347 or 1354-1464 ofNR2A inhibited PMA-induced surface expression ofNR2A ***:First,our data suggests that PMA treatment can induce the surface expression ofhomomeric NR2A ***,this process is probably mediated bythe NR2A C-terminal region between positions 1149 and 1464.

关键词： NMDA receptor NR2A subunit PMA surface expression HEK293 cells

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A new mechanism of regulating surface expression of Glu N2B-containing NMDA receptors by tyrosine phosphorylation site Y1070 within the C-terminus of GluN2B

A new mechanism of regulating surface expression of Glu N2B-...

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中国生理学会第24届全国会员代表大会暨生理学学术大会

作者：罗建红浙江大学医学院神经生物学系

N-methyl-D-aspartate receptors（NMDARs）are heterotetramers mainly composed of two Glu N1 subunits and two Glu N2 or Glu N3 *** composition of synaptic NMDARs is dynamically regulated and mediates critical roles in synaptic *** has been revealed that surface level of Glu N2B-containing NMDARs can be regulated by phosphorylation at Y1472 residue in the Glu N2B C-terminal domain（CTD）by Src family ***,there are several other potential tyrosine sites in the Glu N2B CTD and their function remains to be *** this study,we identified a novel tyrosinephosphorylation site of Glu N2B CTD,Y1070,which was phosphorylated by Fyn in vivo and in *** N2B phosphorylated at Y1070 was mainly located in the postsynaptic *** Y1070F mutation of Glu N2B dramatically decreased the surface expression of Glu N2B-containing NMDARs in a heterogeneous system as well as cultured ***,we found that phosphorylation of Glu N2B at Y1070 increased its association with Fyn,which regulates the phosphorylation of *** is well known that chemical long-term depression decreases the phosphorylation of Y1472 whereas brain-derived neurotrophic factor increases ***,our results showed that a change in the phosphorylation level of Glu N2B at Y1070 is in accord with that of Y1472 under these *** together,Glu N2B Y1070 is functionally regulated and critical for the phosphorylation of its Y1472 by ***,we have uncovered a new mechanism for regulating the surface expression of Glu N2B-containing NMDARs.

关键词： NMDA receptors Glu N2B tyrosine phosphorylation surface expression

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surface expressions of Rayleigh-Taylor Instability in Continental Interiors

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Acta Geologica Sinica(English Edition) 2014年第3期88卷 1004-1016页

作者： WANG Huilin Claire A CURRIE ZHAN Yan University of Alberta State Key Laboratory of Earthquake Dynamics Institute of Geology China Earthquake Administration

Two-dimensional thermal-mechanical numerical models show that Rayleigh-Taylor-type （RT） gravitational removal of high-density lithosphere may produce significant surface deformation （vertical deflection 〉1000 m） in the interior of a continental plate.A reasonable range of crustal strengths and thicknesses,representing a variation from a stable continental interior to a hot orogen with a thick crust,is examined to study crustal deformation and the surface deflection in response to an RT *** general,three types of surface deflection are observed during the RT drip event：（1） subsidence and negative topography; （2） uplift and positive topography; （3） subsidence followed by uplift and inverted *** key factor that determines the surface expression is the crustal *** with a thin crust mainly show subsidence and develop a *** the thick crust models,surface expressions are more variable,depending on the crustal strength and depth of highdensity *** weak crust and a deep high-density anomaly,the RT drip is decoupled from the overlying crust,and the surface exhibits uplift or little deflection,as the RT drip induces contraction and thickening of the overlying *** contrast,with a strong crust and shallow anomaly,the surface is more strongly coupled with the drip and undergoes subsidence,followed by uplift.

关键词： intraplate tectonics Rayleigh-Taylor instabilities surface expression crustal flow

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