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Proxitome profiling reveals a conserved SgT1-NSL1 signaling module that activates NLR-mediated immunity

MOLECULAR PLANT 2024年第9期17卷 1369-1391页

作者： Zhang, Dingliang Yang, Xinxin Wen, Zhiyan Li, Zhen Zhang, Xinyu Zhong, Chenchen She, Jiajie Zhang, Qianshen Zhang, He Li, Wenli Zhao, Xiaoyun Xu, Mingliang Su, Zhen Li, Dawei Dinesh-Kumar, Savithramma P. Zhang, Yongliang China Agr Univ Coll Biol Sci State Key Lab Plant Environm Resilience Beijing 100193 Peoples R China China Agr Univ Coll Agron & Biotechnol State Key Lab Plant Environm Resilience Beijing 100193 Peoples R China Univ Calif Davis Dept Plant Biol Davis CA 95616 USA Univ Calif Davis Coll Biol Sci Genome Ctr Davis CA 95616 USA

suppressor of g2 allele of skp1 (SgT1) is a highly conserved eukaryotic protein that plays a vital role in growth, development, and immunity in both animals and plants. Although some SgT1 interactors have been identified, the molecular regulatory network of SgT1 remains unclear. SgT1 serves as a co- chaperone to stabilize protein complexes such as the nucleotide-binding leucine-rich repeat (NLR) class of immune receptors, thereby positively regulating plant immunity. SgT1 has also been found to be associated with the skp1-Cullin-F-box (SCF) E3 ubiquitin ligase complex. However, whether SgT1 targets immune repressors to coordinate plant immune activation remains elusive. In this study, we constructed a toolbox for TurboID- and split-TurboID-based proximity labeling (PL) assays in Nicotiana benthamiana and used the PL toolbox to explore the SgT1 interactome during pre- and post-immune activation. The comprehensive SgT1 interactome network we identified highlights a dynamic shift from proteins associated with plant development to those linked with plant immune responses. We found that SgT1 interacts with Necrotic Spotted Lesion 1 (NSL1), which negatively regulates salicylic acid-mediated defense by interfering with the nucleocytoplasmic trafficking of non-expressor of pathogenesis-related genes 1 (NPR1) during N NLR-mediated response to tobacco mosaic virus. SgT1 promotes the SCF-dependent degradation of NSL1 to facilitate immune activation, while salicylate-induced protein kinase-mediated phosphorylation of SgT1 further potentiates this process. Besides N NLR, NSL1 also functions in several other NLRmediated immunity. Collectively, our study unveils the regulatory landscape of SgT1 and reveals a novel SgT1-NSL1 signaling module that orchestrates plant innate immunity.

关键词： suppressor of g2 allele of skp1 SgT1 proximity labeling N NLR immune receptor Necrotic Spotted Lesion 1 NSL1 salicylic acid ubiquitination PROgRAMMED CELL-DEATH SCF UBIQUITIN-LIgASE SALICYLIC-ACID DISEASE RESISTANCE PROTEIN INTERACTIONS ESSENTIAL COMPONENT FUNCTIONAL-ANALYSIS CHAPERONE COMPLEX SgT1 TOBACCO

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