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Rheological properties in supernatant of peach gum from almond(Prunus dulcis)

Journal of Central South University 2008年第S1期15卷 509-515页

作者：王森谢碧霞钟秋平杜红岩 School of Resources and Environment Central South University of Forestry and Technology Economic Forest Research and Development Center CAF

The rheological properties in the supernatant of peach gum from Prunnus dulcis were discussed in order to provide more scientific technical parameters and references for developing peach gum as a kind of medicinal *** rheological properties in the supernatant of peach gum were comparatively studied in different material ratios,temperatures,shaking times,pH values and *** results show that,1) the mathematical model of shear rate with material ratio and shear stress is Y=0.069X12+0.035X2 -1.174,R2=0.942;2) the mathematical model of shear rate with temperature and shear stress is Y=4.936X12+0.023 2X2-1.688,R2=0.937;3) the mathematical model of shear rate with shaking time and shear stress is Y=0.005 192 X13-0.140 73X12+1.249 045X1+ 0.036 546 X2-3.644 29,R2=0.954 3;4) the effects of pH value on the rheological properties in the supernatant of peach gum are comparatively complicated with a varying range of 3-11 and the shear rate shows a change trend of saddle model;5) the mathematical model of shear rate with the concentration of NaCl and shear stress is Y=-0.037 44X1+0.012 93 X2,R2=0.998;6) the mathematical model of shear rate with the concentration of CaCl2 and shear stress is Y=0.025 789X1+0.016 19X2,R2 =0.999;and 7) the mathematical model of shear rate with the concentration of sorbic acid potassium and shear stress is Y=0.079 5X1+0.017 3X2,R2=*** results show that the material ratio,temperature,shaking time,pH value significantly affect the rheological properties in the supernatant of peach gum,and the concentrations of NaCl and CaCl2 also significantly affect the rheological properties expect the concentration of sorbic acid potassium.

关键词： peach gum supernatant rheological property

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Effect of Lactobacillus rhamnosus GG supernatant on serotonin transporter expression in rats with post-infectious irritable bowel syndrome

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World Journal of Gastroenterology 2018年第3期24卷 338-350页

作者： Ya-nan Cao Li-juan Feng Yuan-Yuan Liu Kui jiang Mao-jun Zhang Yi-xin Gu Bang-Mao Wang jia Gao Ze-Lan Wang Yu-Ming Wang Department of Gastroenterology and Hepatology Tianjin Medical University General Hospital Department of Functional Division Xingtai People’s Hospital National Institute for Commu-nicable Disease Control and Prevention Chinese Center for Disease Control and Prevention

AIM To evaluate the effect of Lactobacillus rhamnosus GG supernatant(LGG-s) on the expression of serotonin transporter(SERT) in rats with post-infectious irritable bowel syndrome(PI-IBS).METHODS Campylobacter jejuni 81-176(1010 CFU/m L) was used to induce intestinal infection to develop a PI-IBS model. After evaluation of the post-infectious phase by biochemical tests, Dn A agarose gel electrophoresis, abdominal withdrawal reflex(AWR) test, and the intestinal motility test, four PI-IBS groups received different concentrations of LGG-s for 4 wk. The treatments were maintained for 1.0, 2.0, 3.0 or 4.0 wk during the experiment, and the colons and brains were removed for later use each week. SERT m Rn A and protein levels were detected by real-time PCR and Western blot, *** The levels of SERT m Rn A and protein in intestinal tissue were higher in rats treated with LGG-s than in control rats and PI-IBS rats gavaged with PBS during the whole study. Undiluted LGG-s up-regulated SERT m Rn A level by 2.67 times compared with the control group by week 2, and SERT m Rn A expression kept increasing later. Double-diluted LGG-s was similar to undiluted-LGG-s, resulting in high levels of SERT m Rn A. Triple-diluted LGG-s up-regulated SERT m Rn A expression level by 6.9-times compared with the control group, but SERT m Rn A expression decreased rapidly at the end of the second week. At the first week, SERT protein levels were basically comparable in rats treated with undiluted LGG-s, double-diluted LGG-s, and triplediluted LGG-s, which were higher than those in the control group and PBS-treated PI-IBS group. SERT protein levels in the intestine were also comparable in rats treated with undiluted LGG-s, double-diluted LGG-s, and triple-diluted LGG-s by the second and third weeks. SERT m Rn A and protein levels in the brain had no statistical difference in the groups during the *** LGG-s can up-regulate SERT m Rn A and protein levels in intestinal t

关键词： serotonin transporter intestinal infection Lactobacillus rhamnosus supernatant Irritable bowel syndrome

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Impact of Paenibacillus elgii supernatant on screening bacterial strains with potential for biotechnological applications

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Engineering Microbiology 2024年第3期4卷 1-15页

作者： I.C.Cunha-Ferreira C.S.Vizzotto T.D.Frederico J.Peixoto L.S Carvalho M.R.Tótola R.H.Krüger Laboratory of Enzymology Department of Cellular BiologyUniversity of Brasília(UNB)Brasília70910-900Brazil Laboratory of Environmental Sanitation Department of Civil and Environmental EngineeringUniversity of Brasília(UNB)Brasília70910-900Brazil Laboratório de Biotecnologia e Biodiversidade para o Meio Ambiente Universidade Federal de Viçosa(UFV)Viçosa36570-900Brazil

The biotechnological industry faces a crucial demand for novel bioactive compounds,particularly antimicrobial agents,to address the rising challenge of bacterial resistance to current available *** strate-gies for cultivating naturally occurring microorganisms often limit the discovery of novel antimicrobial *** study presents a protocol for targeted selection of bacterial strains using the supernatant of Paenibacillus el-gii,which produces abundant signal molecules and antimicrobial *** samples were inoculated in these enriched culture media to selectively cultivate bacteria resistant to the supernatant,indicating their potential to produce similar *** bacterial strains isolated through this method were assessed for their antibac-terial *** addition,the functional annotation of the genome of one of these strains revealed several gene clusters of biotechnological *** study highlights the effectiveness of using this approach for selective cultivation of microorganisms with potential for biotechnological applications.

关键词： Prospecting supernatant Antibacterial activity

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Phosphorus Recovery as Struvite from the supernatant of Anaerobic Digestion in Wastewater Treatment Plant

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Journal of Environmental Science and Engineering 2010年第1期4卷 14-19页

作者： Gang Xue Yanan Liu Yanqun Wang School of Environmental Science and Engineering Donghua University Shanghai 201620 China

Phosphorus is an irreplaceable and depletable element. Furthermore, it has an almost one-way circulation on earth, so it is necessary to close the phosphorus cycle loop. Phosphorus could be recovered as struvite, which is a good slow-released fertilizer for agriculture. The supernatant of anaerobic digestion used to treat sludge from wastewater treatment plant is one main source from which phosphorus can be recovered. Studies have proven that phosphorus recovery from digester supematant is a feasible choice to preserve phosphorus rock technically and economically. A modified ＂PHOSNIX＂ P-recovery process was applied under the operating conditions of a 9.0 pH value and a 1.8 mg： P ratio with the influent of the centrate coming from the sludge centrifuge of the Songjiang Wastewater Treatment Plant in Shanghai. More than 80% influent phosphorus was recovered as struvite. Crystal products with good purity and low heavy metal content were gained. The largest crystal had a length of up to 0.26 mm. It was found in our study that the reaction time did not play an important role in crystal growth. Therefore, the optimization of the reaction condition for crystal growth should be examined in future study.

关键词： Phosphorus recovery struvite supernatant anaerobic digestion wastewater treatment plant.

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Termite-killing components in Serratia marcescens(SM1)

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Journal of Forestry Research 2021年第4期32卷 1739-1744页

作者： Renjie Fu Jian Luo Kai Feng Xiaoyu Lu Fang Tang Co-Innovation Center for the Sustainable Forestry in Southern China Nanjing Forestry UniversityNanjing 210037People’s Republic of China College of Forestry Nanjing Forestry UniversityNanjing 210037People’s Republic of China

The bacteria,Serratia marcescens(SMI) was previously obtained from the black-winged termite,Odontotermes for mo sanus *** was highly toxic to ***,however,the mechanism of toxicity is *** this study,toxicity test results showed that the main components that affected *** were in a supernatant and that the insecticidal protease in the supernatant resulted in the death of *** addition,zinc sulphate recovery experiments indicated that the metalloproteinases in the supernatant were more *** results provide a theoretical foundation for the future biological control of termites,the basis for the development of pest control technology and the discovery of new pesticides.

关键词： Odontotermes formosanus Shiraki Serratia marcescens strain SM1 supernatant Protease Metalloproteinases

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Nuclear apoptosis induced by isolated mitochondria

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Cell Research 2000年第3期10卷 221-232页

作者： JIANG ZHENG FAN, YUN ZHAO, XIA HONG, ZHONG HE ZHAI (College of Life Sciences, Peking University, Beijing, 100871 China) CollegeofLifeSciences PekingUniversityBeijingChina.

We isolated and purified mitochondria from mouse livers and spinach leaves. When added into egg extracts of Xenopus laevis, they caused nuclei of mouse liver to undergo apoptotic changes. Chromatin condensation, margination and DNA ladder were observed. After incubating isolated mitochondria in some hypotonic solutions, and centrifuging these mixtures at high speed, we got mitochondrial supernatants. It was found that in the absence of cytosolic factor, the supernatant alone was able to induce apoptotic changes in nuclei. The effective components were partly of protein. DNA fragmentation was partly inhibited by caspase inhibitors AC-DEVD-CHO and AC- YVADCHO. Meanwhile, caspase inhibitors fully blocked chromatin condensation. Primary characterization of the nuclear endonuclease(s) induced by mitochondrial supernatants was also conducted. It was found that this endonuclease is different from endonuclease G, cytochrome c-induced nuclease, or Ca2+-activated endonuclease.

关键词： Mitochondria supernatant apoptosis egg ex- tract nuclei DNA cleavage.

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Measurement and analysis of biogas fertilizer use efficiency, nutrient distribution and influencing factors of biogas residues and slurry on pig farms

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International Journal of Agricultural and Biological Engineering 2014年第1期7卷 60-69页

作者： Fan Min Zhu Hongguang Ma Jieqiong Institute of Modern Agricultural Science and Engineering Biomass Energy Research CenterTongji UniversityShanghai 200092China

Although the effects of biogas residues and slurry returning to farmland are good,they still cannot be used widely in *** this study,the biogas fertilizer use efficiency,nutrient distribution and influencing factors of fertilizer use efficiency of biogas residues and slurry in 20 biogas projects in Chongming County,Shanghai,China were measured and *** correlation and a linear regression fit of parts of test indicators were also *** results show that pig farm biogas residues and slurry mixture are nutrient-rich and can be used as a high-quality organic fertilizer,while its fertilizer use efficiency is unstable because of the differences among area,raw materials,fermentation technology and operation *** are not evenly distributed in biogas residues and *** levels of organic matters,P and trace elements were detected in biogas residues and higher levels of water-soluble N and K were detected in biogas *** correlations between some test indicators of biogas residues and slurry mixture are significant,especially between total K and *** regression model fitting results of parts of test indicators are ***,the values of the other test indicators can be estimated by one known indicator which can effectively reduce the determination workload under some limited situations.

关键词： biogas residues and slurry biogas fertilizer fertilizer use efficiency nutrients influence factors environmental protection pollution control supernatant

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Effect of Nickel on Some Aspects of Protein Metabolism in Selected Organs of the Freshwater Mussel Lamellidens marginalis

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Biomedical and Environmental Sciences 1992年第3期5卷 208-220页

作者： P.SREEDEVI B.SIVARAMAKRISHAN A.SURESH K.RADHAKRISHANAIAH Division of Metal Pollution Studies Department of Zoology Sri Krishnadevaraya University Division of Metal Pollution Studies Department of Zoology Sri KrishnadevarayaDivision of Metal Pollution Studies Department of Zoology Sri Krishnadevaraya UniversityDivision of Metal Pollution Studies Department of Zoology Sri Krishnadevaraya University Anantapur 515003 (A.P.) IndiaUniversity Anantapur 515003 (A.P.) IndiaAnantapur 515003 (A.P. India)Anantapur 515003 (A.P.) India

The levels of soluble, structural and total proteins, and the activities of AlAT and AAT decreased along with an increase in the levels of free amino acids and the activity of protease in the ctenidium, hepatopancreas and foot of the freshwater mussel L. marginalis after 1,2,3 and 4 d of exposure to a lethal concentration (115 mg.L-1) of nickel. But the activity of GDH and the level of urea decreased in the hepatopancreas and increased in the ctenidium and foot. A reverse trend was observed in the level of ammonia. In a sublethal concentration (23 mg.L-1), the levels of soluble, structural and total proteins and ammonia decreased in these three organs of the mussel after 1, 5, 10 and 15 d of exposures, with an increase in the *** free aminoacids, urea and in the activities of protease, Al AT, AAT and ODH. The extent of these changes differed in degree depending on exposure period in the lethal and sublethal concentrations. The results are discussed in order to arrive at the degree of metal stress on the overall nitrogen metabolism of the mussel according to the period of exposure to lethal and sublethal concentrations of nickel.

关键词： ammonia lethal Ammonia exposure nickel exposed supernatant Protease organs aquatic

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Propagation of Low Temperature-Adapted Hepatitis A Virus in BSC-1 Cell Culture

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中国医科大学学报 1989年第S2期18卷 54-59页

作者：王兆荃 Stanley M Lemon Infectious Disease Research Unit The Third Affiliated Hospital China Medical University Division of Infectious Diseases University of North Carolina USA.

Hepatitis A virus, HM-175 strain, adapted to low temperature was cultivated in BSC-1 cell *** of the virus was detected by radioimmunoassay(RIA), radioimmunofocus assay (RIFA) and dot blot hybridization. The results showed that, of the 7 low temperature-adapted(LTA) strains studied, strain E, F and D gave the highest titer in BSC-1 cell culture. This culture system was proved to be suitable for the study of the characteristic of temperature mutants of HAV.

关键词： titer Replication adapted cultivated supernatant supplemented enterovirus instead labeled elevated

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STUDY ON PREVENTIVE AND CURATIVE EFFECTS OF LIU WEI DI HUANG TANG ON TUMORS

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Journal of Traditional Chinese Medicine 1994年第3期14卷 207-211页

作者：蔡波文姜廷良 Institute of Chinese Materia Medica China Academy of Traditional Chinese Medicine

Liu Wei Di Huang Tang (LWDHT), a Chinese Prescription for strengthening the body re-sistance, restoring the normal functions of the body to consolidate the constitution, and nour-ishing and invigorating the kidney yin, has been used to prevent and treat severe hypeplasiaof esophageal epithelium for many years. The results of this experiment show that LWDHTcan increase markedly the number of lymphocytes, mainly T lymphocytes, in tumor--bearingmice. Free--flow electrophoresis shows that in tumor--bearing mice the electrophoretic char-acteristics of T lymphocytes are changed, i.e., reduction of the number of T lymphocyteswith a higher electrophoretic rate, but LWDHT can alleviate this disorder. Study on the cellmembrane fluidity of carcinoma cells in EAC mice demonstrates that LWDHT can decreasethe cell membrane fluidity. suggesting that it can inhibit division of carcinoma

关键词： fluidity esophageal epithelium constitution markedly inhibit alleviate prescription supernatant HUANG

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