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Generating supercharged protein ions for breath analysis by extractive electrospray ionization mass spectrometry

Generating supercharged protein ions for breath analysis by ...

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2018年中国质谱学术大会（CMSC2018）

作者： Mufang Ke Jianhua Ding Xingchuang Xiong Fenglei Li Konstantin Chingin Wei Kou Aiying Liu Tenggao Zhu Xiang Fang Huanwen Chen State Key Laboratory of Inorganic Synthesis and Preparative Chemistry College of ChemistryJilin University Jiangxi Key Laboratory for Mass Spectrometry and Instrumentation East ChinaUniversity of Technology National Institute of Metrology

The exhaled breath contains vital information on personal metabolic states, which makes the breath analysis attractive for non-invasive diagnosis.1-3 Analytes in exhaled breath gas are conveniently protonated for extractive electrospray ionization mass spectrometry(EESI-MS) analysis in real time. However, there is still a place for better ionization efficiency. supercharged protein ions produced are extremely efficient proton donors for secondary ionization. High charge states of supercharged protein were found to be the strongest acids, which are able to protonate N2 and Ar in the gas phase.4 This finding opens novel possibilities for EESI to analyze breath metabolites with supercharged protein with enhanced sensitivity. Here, by electrospraying the protein solutions containing 5% 1,2-butylene carbonate, the supercharged protein ions with unusually high proton density were produced as the primary ions for the ionization of exhaled breath samples in the EESI-MS, which resulted in the enhanced ionization efficiency for the breath analytes even with relatively low gas phase basicity. Moreover, the signal enhancement up to 1000 times was achieved for the breath metabolites and the number of metabolites appeared among the mass range of 200-500 Da was increased by about 260%, providing complementary information to the conventional mode of EESI.

关键词： Extractive electrospray ionization ambient mass spectrometry supercharged protein breath realtime analysis

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