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Metabolons,enzyme-enzyme assemblies that mediate substrate channeling,and their roles in plant metabolism

Plant Communications 2021年第1期2卷 13-28页

作者： Youjun Zhang Alisdair R.Fernie Center of Plant Systems Biology and Biotechnology 4000 PlovdivBulgaria Max-Planck-Institut für Molekulare Pflanzenphysiologie Am Mühlenberg 114476 Potsdam-GolmGermany

Metabolons are transientmulti-protein complexes of sequential enzymes that mediate substrate channeling.They differ from multi-enzyme complexes in that they are dynamic,rather than permanent,and as such have considerably lower dissociation constants.Despite the fact that a huge number of metabolons have been suggested to exist in plants,most of these claims are erroneous as only a handful of these have been proven to channelmetabolites.We believe that physical protein-protein interactions between consecutive enzymes of a pathway should rather be called enzyme-enzyme assemblies.In this review,we describe how metabolons are generally assembled by transient interactions and held together by both structural elements and non-covalent interactions.Experimental evidence for their existence comes fromprotein-protein interaction studies,which indicate that the enzymes physically interact,and direct substrate channelingmeasurements,which indicate that they functionally interact.Unfortunately,advances in cell biology and proteomics have far outstripped those in classical enzymology and flux measurements,rendering most reports reliant purely on interactome studies.Recent developments in co-fractionation mass spectrometry will likely further exacerbate this bias.Given this,only dynamic enzyme-enzyme assemblies in which both physical and functional interactions have been demonstrated should be termed metabolons.We discuss the level of evidence for the manifold plant pathways that have been postulated to contain metabolons and then list examples in both primary and secondary metabolism for which strong evidence has been provided to support these claims.In doing so,we pay particular attention to experimental and mathematical approaches to study metabolons as well as complexities that arise in attempting to follow them.Finally,we discuss perspectives for improving our understanding of these fascinating but enigmatic interactions.

关键词： protein-protein interaction metabolon substrate channeling

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Facilitation of cascade biocatalysis by artificial multi-enzyme complexes——A review

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Chinese Journal of Chemical Engineering 2020年第11期28卷 2799-2809页

作者： Xinlei Wei Pingping Han Chun You Tianjin Institute of Industrial Biotechnology Chinese Academy of SciencesTianjin 300308China

Multi-enzyme complexes are the results of natural evolution to facilitate cascade biocatalysis.Through enzyme colocalization within a complex,the transfer efficiency of reaction intermediates between adjacent cascade enzymes can be promoted,resulting in enhanced overall reaction efficiency.Inspired by nature,a variety of approaches have been developed for the assembly of artificial multi-enzyme complexes with different spatial organizations,aiming at improving the catalytic efficiency of enzyme cascade.A recent trend of this research area is the creation of enzyme complexes with a controllable spatial organization which helps with the mechanistic studies and bears the potential to further increase metabolic productivity.In this review,we summarize versatile strategies for the assembly of artificial multi-enzyme complexes,followed by an inspection of the mechanistic studies of artificial multi-enzyme complexes for their enhancement of catalytic efficiency.Furthermore,we provide some highlighted in vivo,ex vivo,and in vitro examples that demonstrate the ability of artificial multi-enzyme complexes for enhancing the overall production efficiency of value-added compounds.Recent research progress has revealed the great biotechnological potential of artificial multi-enzyme complexes as a powerful tool for biomanufacturing.

关键词： Enzyme Biocatalysis Cascade reaction scaffold substrate channeling synthetic biology

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